Gene Information

Name : Kvar_4504 (Kvar_4504)
Accession : YP_003441410.1
Strain : Klebsiella variicola At-22
Genome accession: NC_013850
Putative virulence/resistance : Unknown
Product : adenine-specific DNA-methyltransferase
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0286
EC number : 2.1.1.72
Position : 4773276 - 4774895 bp
Length : 1620 bp
Strand : +
Note : PFAM: N-6 DNA methylase; KEGG: set:SEN4286 type I restriction-modification system methyltransferase

DNA sequence :
ATGGCCAAAGCACCAACCAAAAAAGCCAAAGCAAAGAAAGGCTTTGAAGACACATTATGGGATACCGCGAACCAGCTTCG
CGGCAGCGTTGAATCCTCCGAGTACAAGCACGTGGTGCTGAGCCTCGTGTTCCTCAAATTTATTAGCGATAAGTTTGAAG
CCCGCCGTCAGAAGATGAAAGACGAGGGACAGGGCGATTTCCTTGAGATGGAAGTCTTCTATCAGCAGGACAATATCTTC
TATCTGCCGGAAGAGGCGCGCTGGTCGTTCATCAAGCAGCACGCCAAACAGGATGATATTGCCGTGCGCATTGATACCGC
GCTCTCCACCATTGAGAAGCGCAACCCGACGCTGAAAGGCGCGCTGCCGGACAACTACTTCAGCCGTCAGAATCTGGAAA
CCAAGAAGCTGGCGTCGCTTATCGACACCATCGATAACATCGAAACGCTGGCCCACGAAACCGACGTCGAAGCGCTGTCG
AAAGAAGACCTGGTCGGTCGCGTGTACGAATACTTCCTCGGCAAGTTTGCCGCCACCGAGGGCAAAGGCGGCGGCGAGTT
CTATACGCCAAAATGCGTGGTGACGCTGCTGACCGAAATGCTCGAACCGTTCCAGGGTAAAATTTATGACCCCTGCTGTG
GCTCGGCGGGGATGTTTGTCCAGTCGGTGAAGTTTGTCGAAAGCCATCAGGGGAAAAGCCGCGATATCGCCCTGTACGGT
CAGGAGCTGACGGCCACCACCTACAAGCTGGCGAAGATGAACCTCGCGATTCGCGGCCTTTCCGCCAACCTTGGCGAGCG
CCCGGCGGACACCTTCTTCAGCGACCAGCACCCGGATCTGAAAGCCGACTATATTCTGGCCAACCCGCCGTTTAACCTGA
AAGACTGGCGCAACGACGCCGAGCTGACCAAAGACCCGCGCTTTGCCGGCTACCGCACGCCGCCAACCGGCAACGCCAAC
TACGGCTGGATTTTGCATATGCTTTCCAAGCTGTCGGCCAACGGCACCGCCGGTTTTGTGCTGGCGAACGGCTCGATGAG
CTCCAACACCAGCGGCGAAGGCGAGATCCGCGCGCAGATGATCGAAAACGATCTGATCGACTGCATGATCGCCCTGCCCG
GGCAGCTGTTCTTCACCACCCAGATCCCGGTGTGCCTGTGGTTTATGACCAAGTCGAAAGCGGCCGATCCGGCCAAAGGC
TATCGTGACCGTCAGGGCGAGACGCTGTTTATCGATGCGCGTAACCTTGGCACCATGATCAACCGTACCACCAAAGAGCT
GACGGCGGACGATATCGCCACCATCGCCGATACCTACCATGCCTGGCGCAGCACGCCAGAAGAGCTGGCTGAGCGCGTTA
AGCGCGGCGACAGCAAGCTGGCGCAGTACGAAGATCAGGCCGGATTCTGCAAGGTTGCAACGATTGAAGAGATCAAAGCT
AACGAAAATGTGCTGACCCCGGGCCGCTACGTCGGCGCCGCCGAGCAGGAAGACGACGGCGTTGCGTTTGAAATCAAAAT
GCGCGAGTTATCGCAGACGTTGTTTGCGCAGATGAAGCAGGCGCAAGAACTGGATAACGCGATTCGCCAGAACCTGGAGG
TGTTGGGTTATGGGGAGTAA

Protein sequence :
MAKAPTKKAKAKKGFEDTLWDTANQLRGSVESSEYKHVVLSLVFLKFISDKFEARRQKMKDEGQGDFLEMEVFYQQDNIF
YLPEEARWSFIKQHAKQDDIAVRIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVEALS
KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGMFVQSVKFVESHQGKSRDIALYG
QELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQHPDLKADYILANPPFNLKDWRNDAELTKDPRFAGYRTPPTGNAN
YGWILHMLSKLSANGTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFFTTQIPVCLWFMTKSKAADPAKG
YRDRQGETLFIDARNLGTMINRTTKELTADDIATIADTYHAWRSTPEELAERVKRGDSKLAQYEDQAGFCKVATIEEIKA
NENVLTPGRYVGAAEQEDDGVAFEIKMRELSQTLFAQMKQAQELDNAIRQNLEVLGYGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hsdM BAD24842.1 HsdM protein Not tested Type-V SCCmec Protein 1e-135 52
hsdM YP_251979.1 type I restriction-modification system DNA methylase Not tested SCCmec Protein 5e-137 52
hsdM BAH57701.1 type I restriction-modification system DNA methylase Not tested Type-VII SCCmec Protein 2e-137 52
api49 CAF28523.1 putative HsdM-like N-methyl transferase Not tested YAPI Protein 3e-116 45