Gene Information

Name : Kvar_3756 (Kvar_3756)
Accession : YP_003440668.1
Strain : Klebsiella variicola At-22
Genome accession: NC_013850
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 3944645 - 3946252 bp
Length : 1608 bp
Strand : -
Note : TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase; KEGG: kpe:KPK_3966 enterobactin synthase subunit E

DNA sequence : 
ATGATTGCATTTACCCGTTGGCCGGAAGAGTTTGCGGCCCGCTATCGTCAAAAAGGCTACTGGCAGGATCTGCCGTTAAC
CAATCTGATCACCCGTCACGCGGAAAACGACGCCGTGGCGATTATCGATGGCGAACGGCAGATTAGCTACCGCCAGTTTA
ATCAGCTGGTGGACAACCTTGCCTGCTCGCTGCAGCGCCAGGGACTGAAGCGCGGGGAAACCGCGCTGGTGCAGTTGGGC
AACGTCGCCGAGTTTTACATCACCTTCTTTGCGCTGCTGCGCATCGGCGTCGCGCCAGTGAACGCGCTGTTCAGCCATCA
GCGCAGCGAGCTTAACGCCTACGCCGCGCAGATTAAACCGGCGCTGCTGATTGCCGATCGCGAGCACGCGCTGTTTGCTG
ACGATAGCTTCCTCCATGCGTTTATCGCTGAGCATCCTTCCCTGCGCGTCGCACTGTTGCGTAACGACGGCGGCGAGCGC
GATCTGGCGACGGAGATTAACCGCCCGGCGGATAACTTTGTCGCCAATCCAACCCCTGCCGACGAAGTGGCCTTTTTCCA
GCTCTCCGGCGGCAGCACCGGGACGCCGAAGCTGATCCCGCGCACCCATAACGATTACGACTACAGCATCCGCCGCAGCA
ACGAGATCTGCGGGATCAACGCGGAGACGCGCTACCTCAACGCGCTGCCGGCGGCGCATAACTATGCGATGAGTTCCCCG
GGTTCGCTCGGCGTTTTCCTCGCCGGCGGACGGGTGATCCTCGCTGCCGACCCGAGCGCCACCCTCTGTTTCCCGCTGAT
TGAACAACATCAGATTAACGTCGCGTCGCTGGTGCCGCCGGCGGTCAGCCTGTGGCTGCAGGCTATTCATGAATGGGGCG
GCAACGCGCAACTGCAATCGCTGCAGCTGCTGCAGGTTGGCGGAGCGCGCCTGTCCGCGACGCTCGCCGCGCGCATTCCG
GCGGAAATCGGCTGCCAGCTGCAGCAGGTGTTCGGCATGGCGGAGGGGCTGGTGAATTACACCCGCCTTAACGATAGCCC
GGAGCGGATCATCCATACCCAGGGCTGTCCGATGTGCCCGGACGATGAAGTGTGGGTAGCGGATGCCGATGGCAACCCGC
TGCCGCGCGGCGAAGTGGGCCGCCTGATGACCCGCGGGCCGTACACCTTCCGCGGCTACTACAACAGCCCGCAGCATAAC
GCAGAAGCCTTTGACGCCGACGGGTTTTACTGCTCCGGCGATCTGATTTCGATCGATGAAGACGGGTATATCACCGTCCA
GGGCCGGGAAAAAGATCAGATCAACCGCGGCGGCGAGAAGATCGCCGCCGAAGAGATCGAAAACCTGCTGCTGCGCCATG
AGGCGGTGATCCACGCCGCGCTGGTCAGCATCGAAGACAACCTGCTGGGCGAGAAAAGCTGCGCTTATCTGGTGGTGACC
TCGCCGCTGCGCGCCGTCGCGGTGCGCCGCTTCCTGCGCGAGCAGGGGGTTGCTGAATTTAAACTGCCGGACCGCGTGGA
GTGCGTTGCCGCGCTGCCGCTGACGCCGGTGGGCAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCCGAAGGCAAGC
TGGGCTGA

Protein sequence : 
MIAFTRWPEEFAARYRQKGYWQDLPLTNLITRHAENDAVAIIDGERQISYRQFNQLVDNLACSLQRQGLKRGETALVQLG
NVAEFYITFFALLRIGVAPVNALFSHQRSELNAYAAQIKPALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGER
DLATEINRPADNFVANPTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
GSLGVFLAGGRVILAADPSATLCFPLIEQHQINVASLVPPAVSLWLQAIHEWGGNAQLQSLQLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLNDSPERIIHTQGCPMCPDDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHN
AEAFDADGFYCSGDLISIDEDGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-87 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 3e-87 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-87 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 5e-87 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-87 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 4e-87 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-87 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-87 42
ybtE CAA21394.1 - Virulence HPI Protein 4e-87 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Kvar_3756 YP_003440668.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 0.0 82
Kvar_3756 YP_003440668.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 3e-105 45