Gene Information

Name : cadA (BpOF4_11930)
Accession : YP_003427331.1
Strain : Bacillus pseudofirmus OF4
Genome accession: NC_013791
Putative virulence/resistance : Resistance
Product : cadmium-transporting ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 824076 - 826046 bp
Length : 1971 bp
Strand : +
Note : COG2217 Cation transport ATPase

DNA sequence :
TTGGACGCAGTTAAGGCGGGAGGCTGCTCAAGTTGTGCTCATATGGAAAAGTGTTACGGAACAGAAAGCTGTCAACTTAA
AAAGAACAAAATGAAATTCGTAGAAGAACATTTAGAGTTAACATTAGCACTTATAAGCGGCAGTATCATCATTATGGCTT
GGTTACTGCAAAGATATGAATATCCAGCGCTCTCGGTCGCCCTATTTTTAAGTGCCTATGTCATAGGGGGCTATTTTAAA
GCTAGGGAAGGCATAGAAGATGCACTATCTAATAAAACTTTGAATGTAGAGCTTTTAATGATCTTCGCTGCAATCGGTTC
AGCCTTCATTGGTTATTGGGTGGAAGGCGCAATTTTGATCTTTATTTTTTCTTTAAGCGGGGCTCTTGAAACCTACACGA
TGAATAAAAGTCACCGAGAAATTTCATCCCTTATGGACTTACGACCTGAAACAGCGACTGTTTTGGTCAATGGGAAAGAA
CGTAAAGTAGGGGTAGACCATTTAGTGATTGGTAATTTCGTACTCATAAAACCAGGGGAACGTATACCAGCCGATGGGAA
GATTGTTAAGGGTACATCGATGATTGATGAAGCAGCGTTAAGCGGTGAAGCGCTTCCTCTTCTTAAAACAGTAAATGATG
AAGTATTTACAGGTACAGTAAATAAAAATGGCTTAATTACCATTGAAGTAACGAAAAAAAGCGAGGAGACATTGTTCCAA
AAAATTATAGAACTTGTACAACAAGCGAAAGAGGAAAAAACACCTTCCCAGCAATTTATTGAGAAATTTGAAGGTCCTTA
TGTGAAAATTGTTCTGCTCATTGTAGGATTAATGTTATTTTTACCTCATTATATTCTTGGATGGAGTTGGAGTGAAACAT
GGTACAGAGCCATGGTGATGCTTGTTGTAGCTTCACCCTGTGCTCTAGTGGCTTCAATTATGCCTGCTACATTATCAGCG
ATATCGAATGGAGCGCGTAAAGGAATTCTATTCAAGGGCGGTGTCCATCTTGAAAACTTAGCAGCACTTAAGGCCATTGC
TTTTGATAAAACAGGTACATTAACAAAGGGAAAGCCTTCTGTAACGGATCTTTTTATCCGCCCAGGTTTAGATAAGGAAG
TATTTTTACAAAGTGTGTATATAATTGAAAAGCAATCGACCCATCCATTAGCGGAGGCTGTTGTTCATTATGTGAAGGAT
CATAATATGAAAGATAGAGGCATTCATTCAGCACTCTCTGTTGATTCTATGCATGAAATCCCAGGGTGTGGGGTAGAAGC
TAAGATAAACGATATTCCGTGGAAAGTGGGGAAGCGTAGTTTTATTGGGGATTCTTTGGCCGGCTCTTTTTACGGAAATG
AAGCAAATAGACTTCAAGAGGAAGGGAAAACTCTTGTGTATGTGGCAGATGATAAGGGGGTAGCTGGACTGTTCGCTGTC
CAAGATACGTTGCGTGAAACGACAAAAGAGGCCGTGAAATCATTCAAAGACCAAGGGCTTTATACGATTATGCTCACTGG
AGATCAGGCGGCAACAGCTGCCGCTGTGAAAGAAATGACTCAGATTGATACAGTGGTGGCAGGCTGTTTACCTGAAGGGA
AAGTAATGGAAATCAAAAAGCTTAAAGAAAAGTATCACACTATTGCGATGGTCGGGGATGGAATTAATGATGCACCTGCT
TTGGCTGCGGCAAATATCGGAGTCGCAATGGGAGGAGGTACTGATGCTGCGATTGAAACGTCTGATATCGTTTTAATGAA
AAACGATTTATCAAAAATTGCTGAAGCTGTTCGTTTATCGAAACGAATGAATCGAATTATTAAACAAAATATTGTTTTTT
CTATTGCTGTTATCGCATTGCTCATTATCTCTAATTTTATGGGGCATGTTTCGCTTCCTCTAGGGGTGATTGGACATGAA
GGCAGTACCATTCTTGTGATCTTGAATGGTTTGAGATTATTACGAGGATAA

Protein sequence :
MDAVKAGGCSSCAHMEKCYGTESCQLKKNKMKFVEEHLELTLALISGSIIIMAWLLQRYEYPALSVALFLSAYVIGGYFK
AREGIEDALSNKTLNVELLMIFAAIGSAFIGYWVEGAILIFIFSLSGALETYTMNKSHREISSLMDLRPETATVLVNGKE
RKVGVDHLVIGNFVLIKPGERIPADGKIVKGTSMIDEAALSGEALPLLKTVNDEVFTGTVNKNGLITIEVTKKSEETLFQ
KIIELVQQAKEEKTPSQQFIEKFEGPYVKIVLLIVGLMLFLPHYILGWSWSETWYRAMVMLVVASPCALVASIMPATLSA
ISNGARKGILFKGGVHLENLAALKAIAFDKTGTLTKGKPSVTDLFIRPGLDKEVFLQSVYIIEKQSTHPLAEAVVHYVKD
HNMKDRGIHSALSVDSMHEIPGCGVEAKINDIPWKVGKRSFIGDSLAGSFYGNEANRLQEEGKTLVYVADDKGVAGLFAV
QDTLRETTKEAVKSFKDQGLYTIMLTGDQAATAAAVKEMTQIDTVVAGCLPEGKVMEIKKLKEKYHTIAMVGDGINDAPA
LAAANIGVAMGGGTDAAIETSDIVLMKNDLSKIAEAVRLSKRMNRIIKQNIVFSIAVIALLIISNFMGHVSLPLGVIGHE
GSTILVILNGLRLLRG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
cadA YP_005732869.1 cadmium-translocating P-type ATPase Not tested Type-V SCCmec Protein 2e-105 42
SE0062 NP_763617.1 copper-transporting ATPase copA Not tested SCCpbp4 Protein 1e-105 42
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 1e-99 42
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 4e-101 42
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 4e-101 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA YP_003427331.1 cadmium-transporting ATPase BAC0092 Protein 7e-125 45