Gene Information

Name : pykA (BpOF4_03380)
Accession : YP_003425634.1
Strain : Bacillus pseudofirmus OF4
Genome accession: NC_013791
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2948668 - 2950422 bp
Length : 1755 bp
Strand : -
Note : COG0469 Pyruvate kinase

DNA sequence :
ATGCGTAAAACGAAAATTGTATGTACGATTGGTCCAGCGAGTGAGCAGATTGATACGTTGGTTGAATTAATAGAAGCTGG
AATGAACGTTGCAAGATTAAACTTTTCTCACGGTGATTTTGAAGAACATGGTGCGAGAATTGAGAACATCCGTGAAGCGA
GTAAGCGCACAGGCAAAACAGTAGCGATCTTACTTGATACAAAAGGTCCTGAAATTCGCACACAGACACTAGAAGGCGGC
GTTGCTGAATTAAAAGCAGGTCAAAGCCTTGTTGTCTCAATGGAAGAAGTAGTAGGTAATACAGAGAAGATTTCGATTAC
ATACCCTGGACTTGTTCAAGATGTGCATCCGGGTTCAAAAATCTTGCTTGATGACGGCCTGATTGGATTAGAGGTTGTAG
AAGTTAGAGAGAAAGAAATTGAAACAAAAGTTTTAAACAGCGGTACTCTTAAAAACAAAAAAGGTGTCAACGTACCTAAT
GTAAGTGTGAAACTTCCTGGTATCACCGAAAAAGATACGGCTGATATTAAATTTGGTATTGAACAAGGGGTAGACTTTAT
TGCCGCTTCATTTGTACGCCGTGCATCTGATGTGCTTGAAATTCGTGAACTTCTTGAGCAGAATCAAGCAACTGATATCC
AAATTATCCCTAAGATTGAAAATCAAGAAGGTGTCGACAATATCGATGAAATCCTTGAAGTGTCTGATGGATTAATGGTT
GCTCGCGGAGATTTAGGTGTGGAAATTCCAGCAGAAGAAGTTCCGCTTGTACAAAAAGAATTAATTAAAAAATGTAATGC
AGTAGCAAAACCTGTTATTACAGCTACACAAATGCTTGATTCTATGCAGCGCAACCCTCGTCCGACTCGTGCTGAGGCGA
GTGATGTCGCAAATGCTATTTTTGATGGTACAGACGCGATTATGCTTTCAGGGGAAACAGCAGCTGGTGATTATCCTGTA
GAAGCTGTGCAGACAATGAACAACATTGCTGCTCGTACAGAACAAGCACTAAACTATCAAGCGATTCTTTCTAAGCATAC
AAAAGAAACGCGTCCTTCGATTACAAGTGCAATCAGCCAGTCTGTAGCTCATGCTGCATTTAATTTGAATGCATCAGCTA
TACTCACAGCTACAGAGAGTGGTTACACAGCTCGCGTCGTAGCTAAATACCGTCCAGAGTCTCCAATCATTGCGGTGACA
AGCAATGAGCGTGTTATGCGCACATTAAGCCTTGTATGGGGTGTATTCCCGCTAATGGGGCAAACAGCTCAAACAACAGA
TGAAATGCTCGATACAACGGTAAATACGGCCGTACAAGCTGGTCAAATTGGTCAAGGAGACCTTGTCGTTATTACAGCGG
GTGTTCCTGTAGGCGAAACCGGTACGACGAACTTGATGAAAGTACATGTAATTGGAGAAGTTGTGGCTAAAGGTCAAGGG
ATTGGCCGTCGTTCAGCTTCAGGTAAAGTAGTCGTAGCTCAAAATGCGCAAGAAGCAAATGAAAAGGTAAAAGAAGGTTC
TATTCTTGTTACTTACAGCACAGATAAAGACATGATCCCGGCCTTTGAAAAGGCAGCAGCTGTTATTACAGAAGAAGGCG
GTCTAACGTCTCACGCTGCAGTAGTCGGTTTAAGTCTAAGCACACCTGTGATTGTTGGTGTGGTTAATGCACTTGATCTA
TTCAAAGATGGTGAAGAGATTACAGTAGACGGCGCACGCGGTGACATTTACAGAGGACATGCAAGTGTATTATAA

Protein sequence :
MRKTKIVCTIGPASEQIDTLVELIEAGMNVARLNFSHGDFEEHGARIENIREASKRTGKTVAILLDTKGPEIRTQTLEGG
VAELKAGQSLVVSMEEVVGNTEKISITYPGLVQDVHPGSKILLDDGLIGLEVVEVREKEIETKVLNSGTLKNKKGVNVPN
VSVKLPGITEKDTADIKFGIEQGVDFIAASFVRRASDVLEIRELLEQNQATDIQIIPKIENQEGVDNIDEILEVSDGLMV
ARGDLGVEIPAEEVPLVQKELIKKCNAVAKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDYPV
EAVQTMNNIAARTEQALNYQAILSKHTKETRPSITSAISQSVAHAAFNLNASAILTATESGYTARVVAKYRPESPIIAVT
SNERVMRTLSLVWGVFPLMGQTAQTTDEMLDTTVNTAVQAGQIGQGDLVVITAGVPVGETGTTNLMKVHVIGEVVAKGQG
IGRRSASGKVVVAQNAQEANEKVKEGSILVTYSTDKDMIPAFEKAAAVITEEGGLTSHAAVVGLSLSTPVIVGVVNALDL
FKDGEEITVDGARGDIYRGHASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 7e-112 54
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 6e-112 54
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 6e-112 54
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-111 54

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_003425634.1 pyruvate kinase VFG0479 Protein 3e-112 54