Gene Information

Name : cadA2 (BpOF4_16565)
Accession : YP_003428250.1
Strain : Bacillus pseudofirmus OF4
Genome accession: NC_013791
Putative virulence/resistance : Resistance
Product : cadmium-transporting ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 1697695 - 1699833 bp
Length : 2139 bp
Strand : -
Note : COG2217 Cation transport ATPase

DNA sequence :
ATGACTGAAGCACGAGCCAGAATCTCAGAGGAAGAAAGTAAGACCTATCGTGTTCAAGGATTTTCTTGTGCAGGGTGTGC
CAAAACGTTCGAAGAAAATGTGAAGCATCTGGATGGTGTGTCTGATGCACAAGTGAACTTCGGAGCCTCAAAGATCACGG
TTACAGGAGCAGTCAGTATGAAAGAACTTGAGAAAGCAGGAGCGTTTGAAAACCTCCAGCTTAGAAATGAACACGAAAAA
GCCGCTCCTCACATACCGTTTTGGAAGCAGAAAACGACGATTAAAGTGTATATTGCCATTGCTTTATTACTGATGAGTTT
TCTTTCTAATTATATAGTCGAAGAAGGAAGCTCACTGCCTTTGATGTTGATTATCCTCTCTATCTTAATTGGCGGATATA
CATTGTTTATTAAAGGCCTGAATAACTTAATTCGATTGAAGTTTGATATGAGCACATTGATGACAATTGCCATCATTGGC
GCAGCCTTCATCGGAGAGTGGATTGAAGGCGCTGTTGTTGTCATTTTATTTGCCATTAGCGAGGCGCTTGAGCGATATTC
AATGGATAAAGCAAGACAATCCATTGCCTCACTCATGGATATTGCACCTAATGAAGCACTCATTCGCCGCAGGGAGCAAG
AAATGTTGGTCAATGTAGATGAAATCGAGATCGGTGACATTATGATTGTTAAGCCTGGCCAGAAACTTGCGATGGACGGA
GTTGTCGTAAAAGGCATATCTACCATAAACCAAGCAGCCATTACGGGAGAGAGTGTTCCGGTGATGCGATCGGTGGATGA
TGAAGTGTTTGCTGGGACATTAAATGAAGAAGGTCTGCTTGAAGTGAGAGTGACGAAGCGGGTAGAAGACACGACGATCG
CTAAAATTATTCATTTAGTAGAAGAAGCTCAAGCGGAAAAAGCACCATCTCAAGCATTTGTTGATAAATTTGCCAAATAC
TATACTCCGGCAATCATTATACTCGCGGCACTTATCATTGTCATTCCACCAATGATGCTCGGTGGAGAGTGGAGCGAATG
GATTTACCTTGGTCTTGCCACATTGGTCGTCGGATGTCCTTGTGCCCTTGTTGTCTCCACCCCGATTGCAGTCGTTACAG
CGATTGGGAATGCTGCCCGTAGCGGAGTATTAATCAAAGGCGGTGTTTATTTAGAAGAAGCAGGTGCATTACGTGCGATT
GCTTTTGATAAAACCGGGACATTAACTAAAGGTGTACCAAATGTCACAGAGATCGTTACATTAAAAGGTGAAGAAGCAGA
GACGTTAAAAATTACCGCAGCAATAGAAAAAGGCTCCCAGCACCCGCTTGCTTCAGCTATTATGCGTAAAGCAGAGGATT
CCGGGTTAGACTTTCACTCGGTGCAGGTTGAAGAATTCCAATCCCTTACAGGTAAAGGACTTAAAGCTCAAGTAGATGGT
GTACAGTATTACGTAGGAAGCCCTGCCTTATTTCAGGATCTTCATAAAGAAATCGAATCAACTATACACGACCTCATTAT
CCAGCTGCAAAACGAAGGTAAAACAGTGATGGTCGTAGGGACAAAAAACGAGATTCTCTCCTTAATAGCTGTGGCAGACG
AAGTAAGAGAATCATCTAAAGAGGTCATTCAAAAGCTTAATCAAATAGGTATCGATACAATGATGCTGACGGGCGATAAT
AGAAGAACAGCAGAGGCAATCGGCAAGCAGGTAGGTGTTAAGAACATTGAAGCAGATCTCCTGCCAGAAGATAAGTTAAC
CTATATAAAAGGGCTTAACGATAAGCACCAGCGTGTTGCAATGGTTGGTGACGGGGTTAATGATGCACCGGCCCTTGCTG
CTTCTACTGTCGGAGTAGCGATGGGCGGTGCCGGAACGGATACTGCCCTTGAAACAGCCGATATCGCTCTTATGTCAGAT
GACCTTAGCAAACTTCCTTATACGATTCTATTAAGCCGCAAGGCACTCACGATTATTAAGCAAAACATTACTTTTTCTCT
TGCTATTAAGGCATTTGCTCTACTTTTAATCATACCCGGCTGGCTGACATTGTGGATGGCTATTTTTGCAGATATGGGAG
CGACGCTATTAGTTACACTAAACAGCTTACGCCTAATGAAAATGAATGATAACGAATAA

Protein sequence :
MTEARARISEEESKTYRVQGFSCAGCAKTFEENVKHLDGVSDAQVNFGASKITVTGAVSMKELEKAGAFENLQLRNEHEK
AAPHIPFWKQKTTIKVYIAIALLLMSFLSNYIVEEGSSLPLMLIILSILIGGYTLFIKGLNNLIRLKFDMSTLMTIAIIG
AAFIGEWIEGAVVVILFAISEALERYSMDKARQSIASLMDIAPNEALIRRREQEMLVNVDEIEIGDIMIVKPGQKLAMDG
VVVKGISTINQAAITGESVPVMRSVDDEVFAGTLNEEGLLEVRVTKRVEDTTIAKIIHLVEEAQAEKAPSQAFVDKFAKY
YTPAIIILAALIIVIPPMMLGGEWSEWIYLGLATLVVGCPCALVVSTPIAVVTAIGNAARSGVLIKGGVYLEEAGALRAI
AFDKTGTLTKGVPNVTEIVTLKGEEAETLKITAAIEKGSQHPLASAIMRKAEDSGLDFHSVQVEEFQSLTGKGLKAQVDG
VQYYVGSPALFQDLHKEIESTIHDLIIQLQNEGKTVMVVGTKNEILSLIAVADEVRESSKEVIQKLNQIGIDTMMLTGDN
RRTAEAIGKQVGVKNIEADLLPEDKLTYIKGLNDKHQRVAMVGDGVNDAPALAASTVGVAMGGAGTDTALETADIALMSD
DLSKLPYTILLSRKALTIIKQNITFSLAIKAFALLLIIPGWLTLWMAIFADMGATLLVTLNSLRLMKMNDNE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 0.0 65
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 0.0 63
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 0.0 63

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA2 YP_003428250.1 cadmium-transporting ATPase BAC0298 Protein 3e-114 47
cadA2 YP_003428250.1 cadmium-transporting ATPase BAC0092 Protein 1e-96 43
cadA2 YP_003428250.1 cadmium-transporting ATPase BAC0124 Protein 2e-85 42