Gene Information

Name : Acfer_0557 (Acfer_0557)
Accession : YP_003398271.1
Strain : Acidaminococcus fermentans DSM 20731
Genome accession: NC_013740
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 654113 - 655861 bp
Length : 1749 bp
Strand : +
Note : TIGRFAM: pyruvate kinase; PFAM: Pyruvate kinase barrel; Pyruvate kinase alpha/beta; PEP-utilising protein mobile region; KEGG: bbe:BBR47_13840 pyruvate kinase

DNA sequence :
ATGAAGAAAACAAAAATCATCTGCACCCTGGGGCCGTCCACGGACAAAAAAGAACTGCTGGTGGATATGATCCGCTCCGG
GATGGATCTGGCCCGGTTCAACTTCTCCCATGGCTCCCACAGCGAATGTGAAGCCCGGCTGAACCTGCTGAAGGAAGCGG
AAAAGGAAGCAGGGCGCCTGGTGGGCTATGTGGCCGATACCAAAGGCCCGGAAATGCGTCTGGGGCTGTTCAAAGGGGAC
AAGACCACCCTGGTGCCCGGCCATGAATTCATCCTCACCACGGAGGATGTGGAAGGGACGGCGGAAAAGGCCCATGTGAA
CTACGCCGGTCTGCCGGAAGAAGTGAAGCGGGGGGACACCATCCTGCTCAACGACGGAAAACTGTCCCTGGAAGTCCAGT
CCACCACGGACAAGGAAATCCATACCATCGTGGTGAACGGCGGGGAAATCTCCTCCCGGAAACGGGTGGCAGTGCCCGGG
GCTTTCCTGAAATTGCCCTTCCTGTCCGATGCGGATATTTCCGATATTACCTTTGCCGCCCAGCACGGCATGACCTACGT
GGCCGCCTCCTTTGTGCGGAATGCCCATGACGCCATGGAAATCCGCCGTCTCCTGGAAAAACTGGGGTCCCCCATGGGGA
TCATCGCCAAGATCGAAAACCAGGAAGGGGTGGACAACATCGATGACATCCTCCAGGTGGTGGACGGGGTCATGGTGGCT
CGTGGGGACCTGGGGGTGGAAATTCCCATGGAAGACGTGCCCATTGTCCAGAAGGAAATCATCGCCAAATGCAACGCCCT
GGGCAAGCCGGTGGTCACCGCCACCCAGATGCTGGAAAGCATGATCACCAATTACCGGGCCACCCGGGCGGAAGCCAACG
ATATCGCCAACTCCATCTTTGACGGCACCGACGCCATTATGCTCAGCGGAGAAACCGCATCCGGGGCCTATCCCCTGGAA
GCCGTCCAGACCATGGCCCGGATCGCCAAACGGACAGAAGACGCCATCGACTATGTCACTGTGTTCAAACACAAGGGCAT
CGGGGCCCAGGTCCAGATGACCGATGCCATCAGCCATGCCACCGTGCAGATCGCCCAGGAACTGGAAGCCAACGCCATCA
TGTCCATTACCGAAAGCGGCTACACCGCCCGTCTGGTGGCCAAATACCGTCCCAAAGCCCATGTGCTGGGGGTTTCTCCC
AAAGAGGAAAGCCTGCGGCGGATGAGCTTGTACTGGGGCGTGACACCGCTCAAAGGGGAAAACAAGGCCAGCACCGATGC
GCTGATCGAAGCCTCCCTGAAAAATGCCCTGGACGCAGGGCTGATTAAAAAAGGCGATTCCCTGGTGGTCACCGCCGGCA
TGAACGTGGGAAAGGTGGGCAGCACCAACCTGATCCAGGTGGTCAATGTGGGCCAGAAAATCGTGTCCGGCCAGGGCATC
GGGAAGAAAGCCGCCAGCGGCATCGTCCTGCGGGTGGAAAAGAAAGCGGATCTGGAGCAGTTCAAGCCCGGGATGATCCT
GGCCGTGGCGGCCCTGGAAAATGAAATGGGGACCTGCGCCGCCCAGTCTGGCGGCATCATCGCCGAAGAAGGAGGCTTCA
CCTCTCCCGCCGCCATCATTGGCATCAACTACGGCATCCCGGTGATCGTAGGAGCCAAAGGAGCCATGGAAGCCCTGGAA
ACCGGCGACCTGGTAACCCTGGACGTAACCACGGGGAGCGTGTATGCAGGGAAGATCAATGTGAAATAA

Protein sequence :
MKKTKIICTLGPSTDKKELLVDMIRSGMDLARFNFSHGSHSECEARLNLLKEAEKEAGRLVGYVADTKGPEMRLGLFKGD
KTTLVPGHEFILTTEDVEGTAEKAHVNYAGLPEEVKRGDTILLNDGKLSLEVQSTTDKEIHTIVVNGGEISSRKRVAVPG
AFLKLPFLSDADISDITFAAQHGMTYVAASFVRNAHDAMEIRRLLEKLGSPMGIIAKIENQEGVDNIDDILQVVDGVMVA
RGDLGVEIPMEDVPIVQKEIIAKCNALGKPVVTATQMLESMITNYRATRAEANDIANSIFDGTDAIMLSGETASGAYPLE
AVQTMARIAKRTEDAIDYVTVFKHKGIGAQVQMTDAISHATVQIAQELEANAIMSITESGYTARLVAKYRPKAHVLGVSP
KEESLRRMSLYWGVTPLKGENKASTDALIEASLKNALDAGLIKKGDSLVVTAGMNVGKVGSTNLIQVVNVGQKIVSGQGI
GKKAASGIVLRVEKKADLEQFKPGMILAVAALENEMGTCAAQSGGIIAEEGGFTSPAAIIGINYGIPVIVGAKGAMEALE
TGDLVTLDVTTGSVYAGKINVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-81 43
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-81 43
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-81 43
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-81 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Acfer_0557 YP_003398271.1 pyruvate kinase VFG0479 Protein 5e-82 43