Gene Information

Name : Cwoe_1414 (Cwoe_1414)
Accession : YP_003393217.1
Strain : Conexibacter woesei DSM 14684
Genome accession: NC_013739
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 1483098 - 1484138 bp
Length : 1041 bp
Strand : +
Note : KEGG: pfs:PFLU0706 D-alanine--D-alanine ligase A; TIGRFAM: D-alanine/D-alanine ligase; PFAM: D-alanine--D-alanine ligase domain protein; ATP-dependent carboxylate-amine ligase domain protein ATP- grasp; protein of unknown function DUF201

DNA sequence :
ATGCATAGGCTGAAGACCGGGGTCATCTTCGGCGGCTGCTCCGAAGAGCATCCGATCTCCGTGAAGTCGGCGCAAGAGGT
CGCGCGGCATCTCGATCCCGCGAGATACGAGCCGTTCTACATCGGGATCACGACGAGCGGCGCCTGGAAGCTCTGCGACG
GCCCCGACGCGGATTGGGAGAACGGAAGGTGCCGTCCGGCGACGCTGTCACCCGACAGAAGCGCACGCGGACTGCTCGTC
TTGGAGTACGGACGGTACGAGACGATCAGCCTGGACGTGGTGTTGCCCGTCCTGCACGGCAAGCTCGGGGAGGACGGCGC
GATGCAGGGACTGCTGGAGCTCTCCGGCATTCCGTACGCGGGCTGCGACGTCCAGAGCTCCGCCCTGTGCATGGATAAGT
CCCTGACGTACACGGTCGCCAGAGCCGCGGGAATCGCGACGCCGCGCTTCTGGACCGTCACGGCGAACGAGCACGTCGAT
CCCGACCAGCTCCCCTATCCCGTCTTCGTGAAGCCGGCCCGCTCGGGTTCGTCGTTCGGCGTCAGCAAGGTGTCTGGCAG
GGACGAGCTGCCGGGTGCGCTGGACGTCGCGCGGCGGTACGACTCGAAGGTGCTGGTCGAGGAGGCGGTCGTCGGCAGCG
AGATCGGCTGCGCGATCTTGGGCAACGGTCTGGATCTGCTCGCCGGCGAGGTCGATCAGATCGCGCTGTCGCACGGCTTC
TTCCGGATCCATCAGGAGGACGAGCCGGAGAGAGGGTCCGACAACTCGTCCGTCACGGTTCCCGCGGCCATCTCGCAGGA
GGCACGCGCGCTCGTTCAGGAGACGGCGAAGGCCGCGTATCGCGCCTTGGGATGCCGGGGACTGTCGCGGGTGGACATGT
TCCTGACAGCGGACGGGGACGTGGTCCTCAACGAGGTCAACACGTTGCCCGGCATGACGTCCTACAGCCGGTACCCGCGG
ATGATGGCGGCTGCGGGACTGCCGTTCGCCGAGGTGATCGACCGGATCGTGGCGCTGGCGCTGACGGGTGACGGGCGATG
A

Protein sequence :
MHRLKTGVIFGGCSEEHPISVKSAQEVARHLDPARYEPFYIGITTSGAWKLCDGPDADWENGRCRPATLSPDRSARGLLV
LEYGRYETISLDVVLPVLHGKLGEDGAMQGLLELSGIPYAGCDVQSSALCMDKSLTYTVARAAGIATPRFWTVTANEHVD
PDQLPYPVFVKPARSGSSFGVSKVSGRDELPGALDVARRYDSKVLVEEAVVGSEIGCAILGNGLDLLAGEVDQIALSHGF
FRIHQEDEPERGSDNSSVTVPAAISQEARALVQETAKAAYRALGCRGLSRVDMFLTADGDVVLNEVNTLPGMTSYSRYPR
MMAAAGLPFAEVIDRIVALALTGDGR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 2e-100 61
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 8e-98 60

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 9e-105 63
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase NC_005054.2598280.p0 Protein 1e-103 62
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase NC_014475.1.orf3.gen Protein 1e-103 62
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 2e-100 61
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase AF310956.2.orf5.gene Protein 2e-100 61
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 7e-101 61
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 3e-98 60
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 3e-98 60
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 3e-98 60
Cwoe_1414 YP_003393217.1 D-alanine--D-alanine ligase AF130997.1.orf4.gene Protein 2e-103 60