Gene Information

Name : Kfla_3621 (Kfla_3621)
Accession : YP_003381477.1
Strain : Kribbella flavida DSM 17836
Genome accession: NC_013729
Putative virulence/resistance : Resistance
Product : D-Ala-D-Ala dipeptidase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG2173
EC number : 3.4.13.22
Position : 3894343 - 3894951 bp
Length : 609 bp
Strand : +
Note : PFAM: peptidase M15D vanX D-ala-D-ala dipeptidase; KEGG: sfu:Sfum_0509 peptidase M15D, VanX D-ala-D- ala dipeptidase

DNA sequence :
ATGAGAGCCGGTTTCGCTTTCGTCGACGAGGTGGTGCCCGGACTGCGCTGGGACGCCAAGTACGCGACCTGGGACAATTT
CACCGGCAAGCCGGTCGACGGCTACCTGGTCAATCGGATTGTCGGTACGAACGCTCTGTGCGAGGCCCTGGACACCGCAC
GGGACAAGGCGCAGTCCCTGGGTTTCGGGCTGTTGCTCTGGGACGGATATCGCCCCCAACGCGCCGTGAACTGTTTTCTG
CGCTGGTCGAGGCAGCCGGAGGACGGCCGGACCAAGCTCCGGCACTACCCGAACATCGACAGGACCGAGATGTTCGCGAA
GGGATACGTGTCGGTCCGGTCGGGTCACAGCCGCGGCAGCAGCGTCGACCTGACGCTCTACCACCTAAACACCGGTGAAC
TCGCCGCCATGGGCGGTGACCACGACCTGATGGATCCGATCTCGCACCACGGCGCTCCGGGCCTCACCGCGGCCGAAGCG
CAGAACCGGGAGTCCCTGCGCTCGGTCATGGAGTCCTGCGGCTTCGCTGCGTACGCAGCCGAGTGGTGGCACTACACCCT
CAGGGACGAGCCGTACCCGAACGACTACTTCGACTTTCGCATCGGCTGA

Protein sequence :
MRAGFAFVDEVVPGLRWDAKYATWDNFTGKPVDGYLVNRIVGTNALCEALDTARDKAQSLGFGLLLWDGYRPQRAVNCFL
RWSRQPEDGRTKLRHYPNIDRTEMFAKGYVSVRSGHSRGSSVDLTLYHLNTGELAAMGGDHDLMDPISHHGAPGLTAAEA
QNRESLRSVMESCGFAAYAAEWWHYTLRDEPYPNDYFDFRIG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanXB YP_008394260.1 D-alanyl-D-alanine dipeptidase Not tested Not named Protein 5e-58 64
vanX NP_815950.1 D-alanyl-D-alanine dipeptidase Not tested Not named Protein 1e-58 64

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase U82965.2.orf32.gene. Protein 6e-74 80
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase M97297.gene.p01 Protein 2e-60 67
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase NC_014475.1.orf4.gen Protein 2e-60 67
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase NC_005054.2598281.p0 Protein 2e-60 67
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase AF130997.1.orf5.gene Protein 3e-60 66
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase AF155139.2.orf6.gene Protein 4e-60 66
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase AF310956.2.orf6.gene Protein 2e-58 64
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase AE016830.1.gene2249. Protein 4e-59 64
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase U35369.1.gene7.p01 Protein 8e-59 64
Kfla_3621 YP_003381477.1 D-Ala-D-Ala dipeptidase FJ349556.1.orf5.gene Protein 3e-57 64