Gene Information

Name : Kfla_3320 (Kfla_3320)
Accession : YP_003381180.1
Strain : Kribbella flavida DSM 17836
Genome accession: NC_013729
Putative virulence/resistance : Resistance
Product : Antimicrobial 3''-kinase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG3231
EC number : 2.7.1.87
Position : 3561408 - 3562172 bp
Length : 765 bp
Strand : +
Note : PFAM: aminoglycoside phosphotransferase; KEGG: aby:ABAYE3648 streptomycin 3''-kinase (streptomycin 3''-phosphotransferase) (streptomycin 6- phosphotransferase) (streptomycin 6-kinase)

DNA sequence :
ATGCCTGTGGTGCTGCCGAAGGGGACCTGGGAGCCGGCTGGTTCCGGCGAGTCGGACACCGAGGTGTTCCGGCGCGGCGA
CGGCGCCGTGTACGCGAAGTGCGCCGCGCTGCGGGGCATCGAGGAGCTCCGGGCCGAGCGTGACCGGGTCGTCTGGCTGA
GCGGTACCGGCATCCCCGGCGCCGAGGTCGCCGACTGGATCGAGACCGACGACGGCGCGTGCCTGCTCACGACGGCCGTT
CCGGGCATCGCCGCCGTCGACCTGCCGCCGTCGTCGAGGCTGAAGGCGGTCGAGGTCCTGGCCGAGGCACTGCGGGGACT
GCACGAGCTCGCGGACTGCCCGTTCACCCGCCCGCTGGCCGAGGTGGTCGCGCAGGCGACGGACGTCGTACGCCGCGGTG
CCGTCAACCCCGACTTCCTCACCGACGAGTGGCGGCGCGAGCGCCCCGAGGACCTGCTCGCGCAGATCCAGGCCGACCGC
CCGTACGCCGACGCGCAGGCGGCCCACGACCTGGTGGTCTGCCACGGCGATCCCTGCCTGCCGAACGTGCTGCTCGATCC
CGGCACCCTCGAGATCACCGGCTTCATCGACGTCGGCCGGCTCGGGCTCGCCGACCGGTACGCCGACCTGGCGCTCACGA
CGGCCCAGCTGGGCGACGAGTGGGACCTGGACCCGGCGTCGTTCCACGCCGCGTACGGTCTGGACGCGCCCGATCAGCGG
CGTCTCGTCTTCTACCGCCTGCTCGACTCACTGTCCTGGGGATGA

Protein sequence :
MPVVLPKGTWEPAGSGESDTEVFRRGDGAVYAKCAALRGIEELRAERDRVVWLSGTGIPGAEVADWIETDDGACLLTTAV
PGIAAVDLPPSSRLKAVEVLAEALRGLHELADCPFTRPLAEVVAQATDVVRRGAVNPDFLTDEWRRERPEDLLAQIQADR
PYADAQAAHDLVVCHGDPCLPNVLLDPGTLEITGFIDVGRLGLADRYADLALTTAQLGDEWDLDPASFHAAYGLDAPDQR
RLVFYRLLDSLSWG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
strA YP_005176180.1 aminoglycoside 3''-phosphotransferase protein Not tested ICEPmu1 Protein 6e-49 48
strA AGK07089.1 StrA streptomycin phosphotransferase Not tested SGI1 Protein 4e-50 47
strA AFV47972.1 streptomycin (3') phosphotransferase StrA Not tested AbaR25 Protein 4e-50 47
strA YP_001800932.1 aminoglycoside-3''-phosphotransferase Not tested Not named Protein 6e-50 47
strA AFV48000.1 streptomycin (3') phosphotransferase StrA Not tested delta-AbaR25 Protein 4e-50 47
strA AEQ20909.1 StrA, APH(3')-Ib streptomycin(3') phosphotransferase Not tested Tn6166 Protein 6e-50 47
strA AEZ06049.1 StrA, APH(3')-Ib, streptomycin(3') phosphotransferase Not tested Tn6167 Protein 6e-50 47
strA ACK44539.1 StrA Not tested SGI1 Protein 4e-50 47
strA AEA34675.1 aminoglycoside phosphotransferase Not tested Not named Protein 4e-50 47
strA AFH57207.1 streptomycin resistance protein Not tested AbaR4a Protein 4e-50 47
strA AGK07031.1 StrA streptomycin phosphotransferase Not tested SGI1 Protein 4e-50 47
strA CAJ77025.1 Streptomycin phosphotransferase A Not tested AbaR1 Protein 2e-50 47
ABZJ_00266 YP_005524222.1 hypothetical protein Not tested AbaR22 Protein 6e-50 47
BJAB0868_00286 YP_008211612.1 Aminoglycoside phosphotransferase Not tested AbaR26 Protein 3e-49 47
ABK1_0266 YP_005512838.1 StrA Not tested AbaR4d Protein 6e-50 47
BJAB07104_00282 YP_008207747.1 Aminoglycoside phosphotransferase Not tested AbaR25 Protein 6e-50 47
ABK1_0258 YP_005512830.1 StrA Not tested AbaR4d Protein 8e-50 47

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Kfla_3320 YP_003381180.1 Antimicrobial 3''-kinase AF024602.1.gene5.p01 Protein 3e-50 47
Kfla_3320 YP_003381180.1 Antimicrobial 3''-kinase DQ336355.1.orf1.gene Protein 6e-39 45
Kfla_3320 YP_003381180.1 Antimicrobial 3''-kinase K00432.1.orf2.gene.p Protein 3e-29 42