Gene Information

Name : CtCNB1_0461 (CtCNB1_0461)
Accession : YP_003276503.1
Strain : Comamonas testosteroni CNB-1
Genome accession: NC_013446
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 506504 - 507892 bp
Length : 1389 bp
Strand : +
Note : COG1157, FliI, flagellar biosynthesis/type III secretory pathwayATPase

DNA sequence :
ATGAACCCCTGGCAGCAGTTCATGAGCCAGGCGCGCGCACGCTATGCCGAGCCAGTGCCGCTGGAATGCCAGGGCCGTCT
GACCCGGTTGACCGGCATGGTGCTGGAGGCTTCGGGGCTGCGCGTGCCCGTGGGGGCGCAATGCCATATTCATCAGCATG
GGCAGGAGCCTGTGCTGGCCGAGGTGGTCGGCTTTGCCGGCGAGCGCGCCTTTCTGATGCCTGCGGGCGATATTCAGGGT
TTGTCCAGCGGCGCCATGGTGACGCCGGCAGCCCCCTTTGTTCCCGTGCCCCAGCTGGGCGTGGTGGAGACGGAGAAAGT
CAGCCAGACCGTGGGCGTGCTGCGCCTGCCCATGGGCGACGGCTTGCTGGGGCGGGTGGTGGATTCGCAAGGCACTCCGC
TGGACCATGCGGGCGAGCTGGGCAGCGTGGTGCCGGAGGTGCTGGATCGCAACCCCATCAATGCCATGGACCGGGACCCG
GTGCGCGAGTCGCTGGATACCGGTGTCAAGGCGCTGAACGCGCTGCTGACCGTGGGGCGCGGCCAGCGTCTGGGTCTGTT
TGCCGGCTCGGGTGTGGGCAAGTCCGTGCTGCTGGGCATGATGGCGCGCTACACCCAGGCCGATGTGATCGTGGTGGGTC
TGATCGGCGAGCGTGGCCGCGAGGTCAAGGAGTTCGTCGAGGACATTCTGGGCGCCCAGGACCGCAGCCGTGCCGTGGTC
GTGGCCGCTCCCGCCGATGCGCCGCCGCTGCTGCGCATGCAGGGCGCCAGCTACGCCACGGCAATCGCCGAGCATTTCCG
CGACAAGGGCAAGCATGTGCTGCTGCTCATGGATTCCTTGACCCGCTATGCCATGGCCCAGCGCGAAATCGCCCTGGCCA
TCGGCGAGCCGCCGGCCACCAAGGGCTATCCGCCATCGTGCTTTGCCAAGCTGCCCGCGCTGGTGGAGCGCAGCGGCAAC
GGCCTGCACGGCGTGGGCTCGATCACGGCCTTCTACACCGTGCTGTCCGAGGGCGACGATCAGCAGGATCCGATTGCCGA
TGCGGCCCGCGCCATTCTCGACGGCCACGTCGTGCTCTCGCGGGCTTTGGCCGAGACCGGGCATTACCCGGCCATTGATA
TCGAGCAGTCGGCGTCTCGCGTCATGCACAACGTGGTCTCGCGCGAGCATTTCGAGCTGGCGCGTCGCTTTCGCGCCGTC
TATTCGCGCTATCAGAAGGGACGCGATCTGGTGCAGGTCGGCGCCTATGTCCCAGGCTCCGATCCGCAGTTGGACGAGGC
GATTGCGCTGCAGCCTGCCATGACCGGGTTTCTGCAGCAAAGCATGTTTGAAAGCTCCAGCATGGAGCAAAGCCTGGCCG
GCATGGCGCAAGCCATGCAGCGGGGCTGA

Protein sequence :
MNPWQQFMSQARARYAEPVPLECQGRLTRLTGMVLEASGLRVPVGAQCHIHQHGQEPVLAEVVGFAGERAFLMPAGDIQG
LSSGAMVTPAAPFVPVPQLGVVETEKVSQTVGVLRLPMGDGLLGRVVDSQGTPLDHAGELGSVVPEVLDRNPINAMDRDP
VRESLDTGVKALNALLTVGRGQRLGLFAGSGVGKSVLLGMMARYTQADVIVVGLIGERGREVKEFVEDILGAQDRSRAVV
VAAPADAPPLLRMQGASYATAIAEHFRDKGKHVLLLMDSLTRYAMAQREIALAIGEPPATKGYPPSCFAKLPALVERSGN
GLHGVGSITAFYTVLSEGDDQQDPIADAARAILDGHVVLSRALAETGHYPAIDIEQSASRVMHNVVSREHFELARRFRAV
YSRYQKGRDLVQVGAYVPGSDPQLDEAIALQPAMTGFLQQSMFESSSMEQSLAGMAQAMQRG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 6e-74 47
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-72 47
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-72 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-71 46
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 5e-71 46
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-69 46
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-71 46
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 7e-72 46
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-72 46
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 7e-72 46
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-69 46
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-65 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-65 45
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-65 45
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-65 45
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-65 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 3e-62 44
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-62 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-62 43
escN AAC31513.1 L0034 Virulence LEE Protein 9e-63 43
escN AAK26715.1 EscN Virulence LEE Protein 6e-62 43
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-62 43
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 9e-63 43
escN AAL57542.1 EscN Virulence LEE Protein 6e-62 43
escN YP_003236088.1 translocator EscN Virulence LEE Protein 6e-63 43
escN AAC38383.1 EscN Virulence LEE Protein 4e-63 43
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-62 43
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-62 43
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-62 43
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-58 42
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-63 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 7e-63 42
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-62 42
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 3e-65 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-62 42
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 3e-62 41
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-60 41
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-60 41
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 5e-60 41
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-59 41
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-58 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 1e-58 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-58 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-58 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG2499 Protein 8e-122 64
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG2331 Protein 2e-115 61
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG1254 Protein 4e-111 61
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG0048 Protein 2e-74 47
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG0515 Protein 1e-65 45
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG0390 Protein 6e-72 45
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG0192 Protein 3e-73 45
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG2144 Protein 8e-75 45
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG0730 Protein 3e-63 43
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG0812 Protein 5e-63 43
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG0555 Protein 5e-59 41
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG2459 Protein 2e-58 41
CtCNB1_0461 YP_003276503.1 flagellar protein export ATPase FliI VFG1008 Protein 8e-59 41