Gene Information

Name : Gbro_0620 (Gbro_0620)
Accession : YP_003271841.1
Strain : Gordonia bronchialis DSM 43247
Genome accession: NC_013441
Putative virulence/resistance : Virulence
Product : AMP-dependent synthetase and ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 657421 - 659109 bp
Length : 1689 bp
Strand : -
Note : PFAM: AMP-dependent synthetase and ligase; KEGG: vvy:VVA1306 vulnibactin-specific 2,3- dihydroxybenzoate-AMP ligase

DNA sequence :
ATGCCCGATCAACCGACCGTCGAGTCGACGCCGACAGCTGTGACCACCGGTTTCGTCCCCCGTCCCGCCGACTTCATCGA
CCGGTATCGCGCGCGCGGTTGCTACCTCGACGCCCCACTGTGGCAGTTGTTGGACGACGCCGCGACATCGTCTCCGGACA
GCCCCGCCCTCACCGACCCGGGCGCCCAACCGCCACGCACCTTGACCTACGCAGCACTTCTTGCGGCCACCCGCCGTCGT
GCCGGGGGTTTCCTGCGCGCCGGGCTGCGGCCGGGGCAGCGCGTCGTGGTGCAACAGAACAACACCGCGGAACTCGTCGT
CAACGTGTTCGCCCTGATGCGAGCCGGGTTGCTGCCGGTCATGGCGCTACCGGCGCACCGTCGAGCCGAGATCACCCACC
TTGCACGCACCTCCGGTGCCAGCGCCTACGTGACCGAGGACGGTCGGCACGGCTTCGATCATCGAACGCTGGCAACCGAT
CTCGTCGACGACGTGGAGTCCCTCGCGCACGTCTTCATCGACGGTGACCCAGGCGGGTTCCTGCCCTTGCCGGACGCGGA
CGGCGTCGAGTTGCCGAGCGCCGACGGGATCGACGCGAATCTGCCGGCTCTCTTCCTGATCTCCGGCGGCACCACGGGCC
TGCCCAAGCTGATCCCCCGCACGCACAACGACTACTCGTTCAACGCCCGACGATCCGCCGAGATCGCCGGGTTGGTCGAC
ACCGACGTCTACCTCGTAGCGCTTCCCGGGGCGCATAACTTCCCGCTGTGCTGTCCCGGCATGCTGGGGGTGATCTCGAC
GGGCGGGCACATCGTGCTGGGAACCGATCCGAGTCCCGACGCCGCCTTCGACCTCATCGAGACCTACGGCGTCACGGTGA
CCGCCCTCGTCCCCGCCCTTGCCCAGGTCTGGTGTGCTGCAACGGAATGGGAGCCCGCCGACATCAGTTCGCTGCGTTTG
TTGCAAGTGGGCGGCGCGAAGCTGGCCGAGCCCGACGCCGTCGCGCTCGACGCCGCACTCGGCGACGTCGTGCAGCAGGT
GTTCGGCATGGCCGAGGGACTGATCTGCTACACGCGACTCGACGATCCGCGTGAACTCGTCCACACCACGCAGGGCCGAC
CCATGTCCGACGACGACGAGTTGCGCGTGGTCGATGAGTCGGGTGTCGACGTGCCGGTGGGCACCGAGGGCGAACTGTTG
GTGCGCGGGCCGTACACGATCCGCGGCTACTACCGGGCCGACGACCACAACACCCGGTCGTTCACCGACGACGGCTTCTA
TCGGTCCGGCGACCGCGTACGCGTCCTGCCGTCCGGACACGTCGCCGTGACCGGGCGCATCAAGGACACCATCGTGCGGG
CCGGCGAGAACGTGGCCGCCGACGATGTGGAGGAAAGCCTGCTCGCCCACCCGTCGATTCGCCAGGTGGCGGTCATCGGA
TTACCCGATGACTCACTCGGAGAACGTATCTGCGCGGTCGTGGTACCCACCGACGGGCCGAAATCGCCGGCCGCCGACCT
TCGCACCCTGCGGACCTTCCTCACCGATCAGGGCGTGGCCCCTTTCAAGCTCCCCGATCAGGTGATCGTGGCCACCCGCC
TTCCGGTCACACCGGTCGGCAAGATCGACAAACGTGCGCTGGTCGAGGAGTTCACCGCGGCCCCGCCGACCTCCCCTCCT
GCCGGTTGA

Protein sequence :
MPDQPTVESTPTAVTTGFVPRPADFIDRYRARGCYLDAPLWQLLDDAATSSPDSPALTDPGAQPPRTLTYAALLAATRRR
AGGFLRAGLRPGQRVVVQQNNTAELVVNVFALMRAGLLPVMALPAHRRAEITHLARTSGASAYVTEDGRHGFDHRTLATD
LVDDVESLAHVFIDGDPGGFLPLPDADGVELPSADGIDANLPALFLISGGTTGLPKLIPRTHNDYSFNARRSAEIAGLVD
TDVYLVALPGAHNFPLCCPGMLGVISTGGHIVLGTDPSPDAAFDLIETYGVTVTALVPALAQVWCAATEWEPADISSLRL
LQVGGAKLAEPDAVALDAALGDVVQQVFGMAEGLICYTRLDDPRELVHTTQGRPMSDDDELRVVDESGVDVPVGTEGELL
VRGPYTIRGYYRADDHNTRSFTDDGFYRSGDRVRVLPSGHVAVTGRIKDTIVRAGENVAADDVEESLLAHPSIRQVAVIG
LPDDSLGERICAVVVPTDGPKSPAADLRTLRTFLTDQGVAPFKLPDQVIVATRLPVTPVGKIDKRALVEEFTAAPPTSPP
AG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-94 47
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-94 47
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-94 47
ybtE CAA21394.1 - Virulence HPI Protein 5e-94 47
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-94 47
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-94 47
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-94 47
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-94 47
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-94 47
irp2E YP_005163485.1 siderophore biosynthetic protein Virulence Not named Protein 7e-71 41
irp2E YP_005134497.1 siderophore biosynthetic protein Virulence Not named Protein 6e-71 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Gbro_0620 YP_003271841.1 AMP-dependent synthetase and ligase VFG1817 Protein 5e-112 51
Gbro_0620 YP_003271841.1 AMP-dependent synthetase and ligase VFG0168 Protein 4e-94 47