Gene Information

Name : Gbro_2649 (Gbro_2649)
Accession : YP_003273772.1
Strain : Gordonia bronchialis DSM 43247
Genome accession: NC_013441
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 2834508 - 2835944 bp
Length : 1437 bp
Strand : -
Note : TIGRFAM: mercuric reductase; PFAM: pyridine nucleotide-disulphide oxidoreductase dimerisation region; FAD-dependent pyridine nucleotide- disulphide oxidoreductase; KEGG: pag:PLES_26321 mercuric reductase

DNA sequence :
GTGGCCACGACACAATCCCGTCCGGATCTGGCGATCGTCGGGTCCGGTTCGGCGGCGTTCGCCGCCGCTATCGCCGCGGT
CGGCCACGGCAGGTCCGTGGTGATGATCGAGCGCGACACCGTCGGTGGCACCTGCGTGAACGTCGGGTGTGTGCCGTCGA
AGACGTTGCTGGCCGCCGCGGAGGCCCGCCACGCCGCAGCCGCCGCCGACCGGTTTCCCGGCCTGGGTGCCGCTGCGGTG
CCGCTGGATTTTCCGGGCCTGATCGCCGGTAAGGACGCGCTGGTCGCGCAGCTGCGGGCAAACAAGTACATCGAGCTGGC
GGGCGAGTACGGGTGGGAGATCGTCTCCGGCACGGCAGTGTTCGACGGCACGAGTGCGGCGCCGGGGTTGCGGGTCGGAG
CGAACGACGGCGGGACCCGGGTGATCGAGGCTGGCCATTACCTGATCGCGACCGGTGCGACGCCGTGGATCCCGCCGATC
GACGGTCTGGTCGAGGCCGGGTATCTGACCTCGACCACCGCCATGGACCTGAAGCGGCTCCCGGAGTCGCTGATCGTGAT
CGGCGGCAACACTGTCGGGCTCGAACAAGCCCAGTTGTTCGCCCGCCTCGGCACCCGGGTGACGGTGATCGAGGCCCTGG
GGCGGCTGGCGCCGGGCGAGGAACCGGAAATCTCGGCCGCGATCGAGGCTGTCTTCGCCGATGAGCATATCGCCGTGTTG
ACCGCGGCCACCGTCGAGAAGGTCCTGGCCGGTGGCGGCCACAAGCTGGTGAGCGTGCGCACTCGGGCCGGGGTCAGCGA
GCTGCGGGCCGAACACGTGTTGGTCGCGACCGGTCGCCGCCCGAACACCGCGGGGCTGGGCCTGGAGGCGGTCGGGGTCG
GTGTCGACGGGCGCGGCGCGGTGATCGTCGATGACCGTCAGCGCACCGGCAATGCCCGGATCTGGGCGGCCGGGGACGTG
GCCGGGGGCCCGCAGTTCGTGTATGTGGCCGCCGCGCAGGGAACGCTGGTGGCCGACAACGCGTTCGCCGACGCGGATCG
GGTCCTGGACTACGCCACAGTGCCGCGGCTGACGTTCACCAGCCCCGCCATCGCCGCCGTGGGGCTCACCGAGGCCGAGG
CGGTGACGGCCGGATACCGGTGCCGGTGCCGGACGCTGGCGTTGTCGAGTGTGGCGCGGGCGCTGGTCAATCGCGATACC
CGTGGCCTGGCCAAGATCGTCGCCGACGCCGATACCGGCCGGATCTTGGGCGTGCACGCGCTGGCCGAGGGTGCCGGAGA
CCTGATCACCGCCGCCGGATACGCGATGACCGCGGGTATGACCGTCGACCGGCTCGCCCACAGCTGGGCGCCGTATCTGA
CCATGGCCGAAGCGTTGAAACTGACCGCCCAGACCTTCACCACCGACGTGACGAAACTGTCCTGCTGCGCAGGCTGA

Protein sequence :
MATTQSRPDLAIVGSGSAAFAAAIAAVGHGRSVVMIERDTVGGTCVNVGCVPSKTLLAAAEARHAAAAADRFPGLGAAAV
PLDFPGLIAGKDALVAQLRANKYIELAGEYGWEIVSGTAVFDGTSAAPGLRVGANDGGTRVIEAGHYLIATGATPWIPPI
DGLVEAGYLTSTTAMDLKRLPESLIVIGGNTVGLEQAQLFARLGTRVTVIEALGRLAPGEEPEISAAIEAVFADEHIAVL
TAATVEKVLAGGGHKLVSVRTRAGVSELRAEHVLVATGRRPNTAGLGLEAVGVGVDGRGAVIVDDRQRTGNARIWAAGDV
AGGPQFVYVAAAQGTLVADNAFADADRVLDYATVPRLTFTSPAIAAVGLTEAEAVTAGYRCRCRTLALSSVARALVNRDT
RGLAKIVADADTGRILGVHALAEGAGDLITAAGYAMTAGMTVDRLAHSWAPYLTMAEALKLTAQTFTTDVTKLSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 3e-72 46
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 4e-72 46
merA ABQ57371.1 MerA Not tested SGI1 Protein 2e-54 46
merA AGK07021.1 MerA Not tested SGI1 Protein 2e-54 46
merA AGK07079.1 MerA Not tested SGI1 Protein 2e-54 46
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 2e-53 46
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 1e-52 45
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 9e-53 45
merA AFG30120.1 MerA Not tested PAGI-2 Protein 9e-53 45
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 2e-53 45

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Gbro_2649 YP_003273772.1 mercuric reductase BAC0224 Protein 2e-71 48
Gbro_2649 YP_003273772.1 mercuric reductase BAC0651 Protein 2e-72 46
Gbro_2649 YP_003273772.1 mercuric reductase BAC0653 Protein 3e-52 46
Gbro_2649 YP_003273772.1 mercuric reductase BAC0652 Protein 4e-55 46