Gene Information

Name : Hoch_2019 (Hoch_2019)
Accession : YP_003266457.1
Strain : Haliangium ochraceum DSM 14365
Genome accession: NC_013440
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 2788068 - 2789726 bp
Length : 1659 bp
Strand : +
Note : TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase; KEGG: scl:sce3880 (2,3-dihydroxybenzoyl)adenylate synthase

DNA sequence :
ATGCAACCCGAGCGTCCGAATTCGCCAGCTCTCAAGGGCTGGACCCCGTGGCCGGCGGCGTATGCCGCCGCCTATCGCCG
CGCCGGCTACTGGCGCGGCGATACCTTTGACAAGATGCTGCGCGAGCGCGCCCAGGAGCACGGCGAGCGCGAGGCCGTGG
TCGATGGCCTGCGACGCCTGAGCTACCGTGCGCTCGATGAGCGGGCTTCGCGTCTGGCGGTGGGATTTCGGACGCTCGGC
ATCGAGCCTGGCGCGCGCGTGGTGGTGCAGCTCCCCAACCGCGTCGAGTTCTTCGAGGTGTGTTTCGCGCTGTTCCGCCT
GGGCGCGCTGCCGGTGATGGCGCTGCCCGCGCACCGCCGGGTCGAGATCGCGTATTTCTGCCAGTTCACCGAGGCCAGCG
CCTACGTTATCGCCGACCGCGTGGCCGGGTTTGACTATCGCGCCCTGGCCGACGAGGTGAACGCCGAGACCCCAAGCCTG
CGACACGTCATCGTCGTGGGCGAGGCCGGCGAGCACACGGCCCTCGAGTCGCTGGTGGCACAGGGCCCGCTGCCGGCGCC
GGACTGGCCCGCGCCCGCGCCCGCAGACGTGGCGTTTTTTCAGCTCTCGGGCGGCAGCACAGGCGTGCCCAAGCTCATCC
CGCGCACCCACGACGACTATCTCTACAGCGTGCGCGCCAGCGCCGAGATCTGCCGACTCGATCGCGACAGCGTGTACCTG
GCCGCGCTGCCGGCGGCGCACAATTTCACCCTCAGCTCGCCCGGCAGCCTGGGCGCGCTGTATGCGGGCGGGCGCGTGGT
GCTTTGCGAGCGACCCAGCCCCGACGACGCCTTTCCGCTCATCGAGCGCGAGCGCGTGAGCATCACCGCGCTGGTGCCAC
CGCTGGTGTCGGTGTGGCTCGACGCGGCCCGCGCCGGACGCAACGATCTCTCCAGCCTGCAGGTGTTGCAGGTGGGCGGC
GCCAAGCTCGGCGCCGAGCTGGCGCGGCGCGTGCGTCCGGGCCTGGGTTGCAGCTTGCAGCAGGTCTTCGGCATGGCCGA
GGGCCTGGTCAACTACACGCGCCTCGACGACCCCGAGGAGCTCATCGCCGAGACCCAGGGGCGTCCGATCTCGCCGGATG
ACGAGATCCGCGTGGTCGACGACGACGATCGCGAGCTCGAGATCGGACAAGTGGGCCATTTGCTCACGCGCGGACCGTAC
ACCATTCGCGGCTACTACAAGGCCGATGCGCACAACGCGCGCGCGTTCACCAGCGACGGCTTCTACCGCACCGGCGACCT
GGTGCGGCTCACGCCCGAGGGCTATCTGGTGGTCGAGGGCCGGGCCAAGGACCAGATCAATCGCGGCGGCGAAAAGATCG
CGGCCGCCGAGATCGAGAACCATCTGCTCGCCCACCCCGAGGTGCTCGACGCCGCCATCGTCGCCATGCCCGACGCGTTT
CTCGGCGAGCGTTCGTGTGCCTTCGTCATCGCCCGCGACCAGCCGCCGCGCGCACGCGCGTTGCTCGCCTTTCTACGCCA
GCGCGGCCTGGCCGCCTTCAAGATTCCCGACCGCGTCGAATTCGTCGACGCCTTTCCCAAGACCAGCGTCGGCAAGGTCG
ACAAACGCGCGTTGCGCGCTCGCATCACCGAGCTGCTCGCGGCCCGCGCGTCGGCCTGA

Protein sequence :
MQPERPNSPALKGWTPWPAAYAAAYRRAGYWRGDTFDKMLRERAQEHGEREAVVDGLRRLSYRALDERASRLAVGFRTLG
IEPGARVVVQLPNRVEFFEVCFALFRLGALPVMALPAHRRVEIAYFCQFTEASAYVIADRVAGFDYRALADEVNAETPSL
RHVIVVGEAGEHTALESLVAQGPLPAPDWPAPAPADVAFFQLSGGSTGVPKLIPRTHDDYLYSVRASAEICRLDRDSVYL
AALPAAHNFTLSSPGSLGALYAGGRVVLCERPSPDDAFPLIERERVSITALVPPLVSVWLDAARAGRNDLSSLQVLQVGG
AKLGAELARRVRPGLGCSLQQVFGMAEGLVNYTRLDDPEELIAETQGRPISPDDEIRVVDDDDRELEIGQVGHLLTRGPY
TIRGYYKADAHNARAFTSDGFYRTGDLVRLTPEGYLVVEGRAKDQINRGGEKIAAAEIENHLLAHPEVLDAAIVAMPDAF
LGERSCAFVIARDQPPRARALLAFLRQRGLAAFKIPDRVEFVDAFPKTSVGKVDKRALRARITELLAARASA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-104 52
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-104 52
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 1e-104 52
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-104 52
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-104 52
ybtE CAA21394.1 - Virulence HPI Protein 1e-104 52
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-104 51
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-104 51
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-104 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Hoch_2019 YP_003266457.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 1e-125 57
Hoch_2019 YP_003266457.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 2e-107 52
Hoch_2019 YP_003266457.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 4e-99 49