Gene Information

Name : Hneap_0436 (Hneap_0436)
Accession : YP_003262340.1
Strain : Halothiobacillus neapolitanus c2
Genome accession: NC_013422
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 457843 - 459240 bp
Length : 1398 bp
Strand : +
Note : KEGG: tgr:Tgr7_1966 flagellar biosynthesis/type III secretory pathway ATPase, FliI/YscN; TIGRFAM: flagellar protein export ATPase FliI; ATPase, FliI/YscN family; PFAM: H+transporting two-sector ATPase alpha/subunit beta central region; H+transporting two-

DNA sequence :
ATGACGGCGGGCCGCAATCCCGCTGTGTTCGATCCACCTTCTATGCGCGTCGGCGGAGTTGGGTTGGACTGGGCCCGTCA
CCTCATGGGCTTGCAGGCACGCGTTCAACGCCCGCCGCTGCAAGCCGAAGGGCGAATCGTGCGGGTTGTGGGCTTGGCGC
TCGAAGCCAGCGGGCTTACAGCGGCCATTGGCGACCAATGCCGTATTTTTCTGGATGATGATCCGGCCGGTCGTTTTGTG
GATGCCGAAGTGGTCGGTTTTGCCGAGCGCAACACCTACCTCATGCCACTGGAAGCGCTGAGGGGTATCGCGCCGGGTTG
TCGGGTGCAGAATCAGGGTGCATCCATTCGGATGCCGCTGGGGCCGGAGTTGCTGGGGCGTGTTCTGGATGCGCGTGGAC
AACCTCTGGACGATGGCCCGGCCATTTTTGGCGACATCCCTGTTGATTTGCAGGGTCGAAGCAACAATCCGCTCAAACGT
CGCCCGATCCGTGAACCACTGGATGTGGGTGTGCGGGCGATCAATGCCCTGCTCACCGTGGGGCGTGGGCAGCGAATGGG
TATTTTTGCCGGTAGTGGCGTGGGTAAAAGCGTCCTGCTTGGCATGATGACACGCTACACCACGGCGGAGATTATCGTGG
TCGGCCTGATCGGCGAGCGGGGGCGCGAGGTGCGTGAATTTGTCGCCGAGATTCTCGATGACGAAGCGCGATCAAGGGCG
ATTGTGATCGCCGCCCCGGCCGATGCTTCGCCGCTCATGCGTTTGCACGGTGCGAGTCTGGCGACGGCCATCGCCGAATA
CTTTCGCGAGCAGGGCCATGATGTGTTGCTGTTGATGGATTCTTTGACGCGTTACGCGCAGGCTCAGCGGGAAATCGGTT
TGGCGATTGGCGAGCCACCGGCCACCAAGGGGTATCCGCCGTCGGTATTCGCCCGTTTGCCTGCCCTGGTTGAACGTGCG
GGTAATGGGGAATCGGCCCGCGGTAGCATGACGGCGTTTTATACCGTGCTGGTGGAAGGGGATGATCAAAACGACCCCAT
CGCCGATGCCGCGCGCGCCATCCTTGATGGGCACATCGTGCTCTCTCGTGCCATTGCCGAACGGGGCCGTTATCCGGCCA
TTGATATCGAAGCCTCCATCTCCCGACTGATGCCCGCGCTGGTATCACCCGAGCATCTGGATTTGATGCAGCGCTTCAAG
CAATTGATGAGCACGTACAACCAAAGCCGGGACTTATTAGCCGTTGGCGCGTATCGTCGGGGGCATGATGTACGGCTTGA
TCGGGCCATTGATGCGCAGCCTGATCTGGAGGCGTTCATTAGTCAGTCCATCCATGAATCGGCAGACCTTGATTCGAGCT
TTGCGGCGCTTGAATCATTGATGCATAGCGCCGCCTGA

Protein sequence :
MTAGRNPAVFDPPSMRVGGVGLDWARHLMGLQARVQRPPLQAEGRIVRVVGLALEASGLTAAIGDQCRIFLDDDPAGRFV
DAEVVGFAERNTYLMPLEALRGIAPGCRVQNQGASIRMPLGPELLGRVLDARGQPLDDGPAIFGDIPVDLQGRSNNPLKR
RPIREPLDVGVRAINALLTVGRGQRMGIFAGSGVGKSVLLGMMTRYTTAEIIVVGLIGERGREVREFVAEILDDEARSRA
IVIAAPADASPLMRLHGASLATAIAEYFREQGHDVLLLMDSLTRYAQAQREIGLAIGEPPATKGYPPSVFARLPALVERA
GNGESARGSMTAFYTVLVEGDDQNDPIADAARAILDGHIVLSRAIAERGRYPAIDIEASISRLMPALVSPEHLDLMQRFK
QLMSTYNQSRDLLAVGAYRRGHDVRLDRAIDAQPDLEAFISQSIHESADLDSSFAALESLMHSAA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-87 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-88 48
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 9e-88 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-89 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 6e-73 48
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-73 48
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-73 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-73 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-73 48
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 9e-75 47
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 7e-87 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-88 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-88 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-88 47
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 47
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 8e-73 46
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 8e-77 46
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 5e-73 46
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-73 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-72 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-72 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-78 45
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-79 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 5e-79 45
escN AAC38383.1 EscN Virulence LEE Protein 6e-79 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 5e-79 45
escN AAK26715.1 EscN Virulence LEE Protein 6e-79 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-79 45
escN AAL57542.1 EscN Virulence LEE Protein 6e-79 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 4e-79 45
escN AAC31513.1 L0034 Virulence LEE Protein 4e-79 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-79 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 4e-79 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-76 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-76 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-70 44
lscN AAO18044.1 LscN Virulence TTSS locus Protein 8e-82 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-73 44
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-77 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-71 43
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-68 43
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-68 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG1254 Protein 4e-122 61
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG2331 Protein 2e-114 60
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG2499 Protein 4e-125 59
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG0515 Protein 3e-73 48
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG0048 Protein 4e-88 47
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG2144 Protein 1e-86 47
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG0192 Protein 1e-85 45
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG0812 Protein 2e-79 45
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG0730 Protein 4e-79 45
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG0390 Protein 1e-85 44
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG1778 Protein 8e-68 42
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG2459 Protein 9e-71 41
Hneap_0436 YP_003262340.1 flagellar protein export ATPase FliI VFG1008 Protein 2e-64 41