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Name : lysS (Pecwa_0865) Accession : YP_003258291.1 Strain : Pectobacterium wasabiae WPP163 Genome accession: NC_013421 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 919283 - 920800 bp Length : 1518 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGGCTGAATCACAATTACAGGGTGCCGATCAGGCGCAAGATCTGAATAATGAATTAAAAACGCGTCGTGAAAAGCTGGT GACGCTGCGTGAAAACGGGATCGCATTCCCGAATGATTTCCGCCGTGACAGCACCTCCGATCGTCTGCACGCTGAGTTCG ATGCCAAAGAGAATGAAGAACTGGAAGAACTGGGCATTGAAGTGACCGTTGCAGGCCGTATGATGACGCGTCGCATTATG GGTAAAGCCTCTTTCGTAACCTTGCAGGACGTCGGTGGCCGTATCCAACTGTATGTTTCCCGCGACGATCTGGCGGAAGG CATCTATAACGAGCAGTTCAAGAAGTGGGATCTGGGCGATATTCTGGGTGCACGTGGCAAGCTGTTCAAAACCAAAACGG GCGAGCTGTCGATTCACTGCACCGAACTGCGCCTGCTGACCAAAGCGCTGCGTCCGCTGCCGGATAAATTCCACGGTCTG GCCGATCAGGAAACCCGCTATCGTCAGCGCTATTTGGATCTGATCGCTAACGACGAATCCCGCAACACCTTCCGTATCCG TTCTAACGTGATGGCGGCTATCCGCCGCTTCATGGTAGATCACGGCTTTATGGAAGTGGAAACGCCGATGATGCAGGCGA TCCCCGGCGGTGCGTCTGCTCGTCCGTTCATCACGCACCATAACGCGCTGGACATCGATATGTACCTGCGTATCGCACCG GAACTGTACCTGAAGCGTCTGGTTGTCGGTGGTTTTGAGCGCGTGTTTGAGATCAACCGTAACTTCCGTAACGAAGGCAT TTCCCCACGTCACAACCCTGAGTTCACCATGATGGAACTTTATATGGCGTATGCCGATTATAAAGACCTGATTGTGTTGA CGGAAAACCTGTTCCGCACGCTGACGCAGGACGTGCTGGGTTCGACAACAGTGGTGTACGGCGATCAGACATTCGACTTC GGTAAGCCGTTTGAAAAACTGACGATGCGCGAAGCGATCTGCAAATACCGTCCTGAAACCAACGTTGCCGATCTGGACTG CCTGGAGAAAGCGACCGCGATCGCTCAGTCTCTGGGCATCAAGATCGAGAAAAGCTGGGGATTGGGTCGCATCGTTACCG AGATCTTCGAAGAAACGGCAGAAAGCAGCCTGATCCAACCGACCTTCATCACCGAATATCCGGCGGAAGTGTCGCCGCTG GCGCGTCGTAACGATCAGAACCCGGAAATTACCGATCGCTTTGAGTTCTTCATCGGCGGCCGTGAAATTGGTAACGGCTT CTCCGAACTGAATGACGCCGAAGATCAGGCTGAGCGTTTTGCCCAGCAGGTCAATGCGAAAGATGCAGGCGATGACGAAG CGATGTTCTACGATGAAGACTATGTGACAGCGCTGGAGCACGGTCTGCCGCCGACTGCGGGTCTGGGTATTGGTATCGAC CGTATGGTGATGTTGTTCACTAACAGCCACACCATTCGCGACGTCATTCTGTTCCCGGCGATGCGTCCGCAGAAGTAA Protein sequence : MAESQLQGADQAQDLNNELKTRREKLVTLRENGIAFPNDFRRDSTSDRLHAEFDAKENEELEELGIEVTVAGRMMTRRIM GKASFVTLQDVGGRIQLYVSRDDLAEGIYNEQFKKWDLGDILGARGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL ADQETRYRQRYLDLIANDESRNTFRIRSNVMAAIRRFMVDHGFMEVETPMMQAIPGGASARPFITHHNALDIDMYLRIAP ELYLKRLVVGGFERVFEINRNFRNEGISPRHNPEFTMMELYMAYADYKDLIVLTENLFRTLTQDVLGSTTVVYGDQTFDF GKPFEKLTMREAICKYRPETNVADLDCLEKATAIAQSLGIKIEKSWGLGRIVTEIFEETAESSLIQPTFITEYPAEVSPL ARRNDQNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFAQQVNAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID RMVMLFTNSHTIRDVILFPAMRPQK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 6e-166 | 68 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 3e-88 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_003258291.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 3e-166 | 68 |
