Gene Information

Name : entE (Pecwa_0561)
Accession : YP_003257996.1
Strain : Pectobacterium wasabiae WPP163
Genome accession: NC_013421
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 597069 - 598697 bp
Length : 1629 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGAGCATTGAATTTACGCCCTGGCCAGAGGAACTGGCACAGCGTTATCGCGACAAGGGGTACTGGGTTGGCTTGCCGTT
AACGGATGCCTGGGAACGCCATCTGACGACACAGCCGGATGCGGTTGCCGTCGTTTGTGGCGAACGTCAGTGGAGCTATC
GAGAGCTGGACCAACAGTCTTCCGCGCTGGCATCGCGCCTGACGGAAAGCGGCCTACGCTGTGGTGACACTGCGCTGGTA
CAACTGCCGAACGTCGGCGAATTTTATCTGACCTTTTTTGCGTTGCTGAAAATGGGCGTCGCGCCAGTTAACGCGTTGTT
CAGCCATAACAAACTGGAACTGCTGTCGTATGCCACGCAGATCGAACCGCGTTTGCTGATCGCATCGGCCGAACATCCAC
TGTTTGGCAACGGTGAATTTCTGGATCGGCTACAAACGCAGGTTCCTGGCCTGCAAACGGTGGTGATGCTTGGCGACAGC
CCGCTGGGGCACCGCCTGTCTGACTGGCTGCAACTACCTGCTTCAACCAGCGAATATCAGCCTTCCGCATCGGGACAGGT
CGCCTTTTTCCAGCTTTCCGGCGGCAGTACCGGCACGCCGAAGCTGATTCCCCGTACCCATGATGATTACTACTACAGCG
TGCGCCGTAGCGTGGAAATTTGTGAGCTAACGCCACAAACCCGTTACCTGTGCGCGTTGCCAGCACCGCATAATTTCCCG
TTAAGCTCGCCCGGTTCGCTTGGCGTGTTTTATGCCGGTGGGCGCGTGGTGCTGGCGTCCGATCCGGGGGCGATGACCTG
CTTCCCGCTGATCGAACGACATCAGATCGATATTACCTCGCTGGTGCCGCCTGCTGCCGCGTTGTGGATCCAGGCGGCAG
AACAGTTTGGCGCGGCGCTGCGTAGCCTGAAAGTCTTGCAGGTGGGGGGCGCAAAGCTAAGTGAAACCGTCGCCCGACGC
ATCCCGGCGGTCTTGGGTTGCCAGCTACAGCAGGTGCTTGGGATGGCGGAAGGGCTGGTGAACTACACCCGACTGGATGA
CAGCGATGAACATACCTTTACCACGCAAGGCTGCCCGATGAGCCCGGACGATGAAGTGAAGGTGTTAGACATAGAGGGTA
ATCCGGTGCCGAGGGGCGAAGCGGGGCTGCTGGCGACGCGTGGCCCGTACACCTTCCGTGGCTATTACCGCAGCCCGGAG
CACAATGCCCGTGCTTTCGACAGTGAGGGTTTCTATCATTCGGGCGATGTAGTGCAGATGACCGAAGAAGGCTATTTGCG
CGTGGTTGGGCGGGAGAAAGATCAGATTAACCGAGGTGGTGAAAAAATCGCTGCCGAAGAGATTGAAAACCTGTTGCTCA
AGCATGACGGCATTCTCCATGCTGCGCTGGTATCCATGCCCGATCCGGTCATGGGCGAAAAGAGCTGCGCTTTTCTGGTG
GTCAGCGATCCCTCTCTGAAAGCCATCGCGTTAAGAAAATATCTTCGTAATCAGGGGATTGCTGAATTCAAACTGCCGGA
TCGCTTTGAGATGATCGATACCTTGCCCGTCACGCCGGTCGGCAAGATTGATAAGAAATTACTCCGTCAGCGCATTCAGG
CGCAACTGAGCATTCAAGACCCAACGTAG

Protein sequence :
MSIEFTPWPEELAQRYRDKGYWVGLPLTDAWERHLTTQPDAVAVVCGERQWSYRELDQQSSALASRLTESGLRCGDTALV
QLPNVGEFYLTFFALLKMGVAPVNALFSHNKLELLSYATQIEPRLLIASAEHPLFGNGEFLDRLQTQVPGLQTVVMLGDS
PLGHRLSDWLQLPASTSEYQPSASGQVAFFQLSGGSTGTPKLIPRTHDDYYYSVRRSVEICELTPQTRYLCALPAPHNFP
LSSPGSLGVFYAGGRVVLASDPGAMTCFPLIERHQIDITSLVPPAAALWIQAAEQFGAALRSLKVLQVGGAKLSETVARR
IPAVLGCQLQQVLGMAEGLVNYTRLDDSDEHTFTTQGCPMSPDDEVKVLDIEGNPVPRGEAGLLATRGPYTFRGYYRSPE
HNARAFDSEGFYHSGDVVQMTEEGYLRVVGREKDQINRGGEKIAAEEIENLLLKHDGILHAALVSMPDPVMGEKSCAFLV
VSDPSLKAIALRKYLRNQGIAEFKLPDRFEMIDTLPVTPVGKIDKKLLRQRIQAQLSIQDPT

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 5e-88 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-88 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-88 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-88 41
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 1e-88 41
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-88 41
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-88 41
ybtE CAA21394.1 - Virulence HPI Protein 1e-88 41
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 7e-89 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_003257996.1 enterobactin synthase subunit E VFG0932 Protein 2e-166 64
entE YP_003257996.1 enterobactin synthase subunit E VFG0168 Protein 2e-109 46
entE YP_003257996.1 enterobactin synthase subunit E VFG1817 Protein 2e-88 41