Gene Information

Name : fliI (GYMC61_2006)
Accession : YP_003253104.1
Strain : Geobacillus sp. Y412MC61
Genome accession: NC_013411
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 2019221 - 2020489 bp
Length : 1269 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
TTGTATAAACGGTTTGGCAAAGTGTCGCGCGTCATCGGCTTGATGATGGAATCGAAAGGACCGAAAAGCTCCATTGGCGA
TCTTTGTTACATCCATGAACAGAGCGGATGCCGGAAAATTCCCGCCGAAGTCGTCGGCTTTCATGAACAGCACGTCTTGC
TTATGCCGTTTTCCGCCACGACCCATATCGCTCCCGGCTGCCTTGTCGAAGCGACCGGCCGGCCGCTTGAAGTGCACGTC
GGCGATGCGCTCATTGGCATGGTTCTTGATCCGCTCGGCCAACCGCTTGACGGGAGCCCGTTGCCGCCCGGGCTGAAGCC
GGTTTCGGTCGAACGCGATCCGCCCAATCCGCTCGTTCGGCCGCCGATTGTCGAACCGCTTGAAGTCGGCATCCGCGTCA
TCGACAGCCTGCTTGCCGTCGGCAAGGGGCAGCGCGTCGGCATTTTTGCCGGCTCGGGCGTCGGCAAAAGCACGCTCATG
GGAATGATCGCCCGCCATACGTCGGCTGATGTGAACGTCATCGCCTTAATCGGCGAGCGTGGCCGCGAAGTGCGCGAGTT
TTTAGAGCGCGACCTTGGCCCGGACGGCTTGGCCCGCTCGGTCGTCGTCGTCGCCACGTCCGACCAGCCGGCGCTGATGC
GCGTCAAAGGCGCCTATACGGCAACGGCGATCGCCGAATATTTCCGCGACCAAGGGGCGGATGTCATGCTCATGATGGAC
TCGGTCACCCGCGTCGCGATGGCGCAGCGGGAGATTGGCCTTGCCGTCGGTGAGCCGCCGACGACGAAAGGCTATACGCC
ATCGGTCTTCGCCCTCCTGCCAAAGCTGCTTGAACGGGCTGGAACGAACGCTTCCGGCACGATCACCGCGTTTTATACAG
TGCTTGTTGACGGCGATGACATGAATGAGCCGATCGCGGATGCGGTGCGCGGCATTTTGGATGGCCATTTTGTATTGGAA
CGCCAGCTGGCGAACAAAGGGCATTACCCGGCGATTAACGTGCTCAAAAGCGTCAGCCGCGTCATGCCCCACATGATCAG
CGCAGAGCACCGCGAAGCGGCAAACCGGTTTCGCCGCCTTCTGTCCACTTATATCGATTCGGAGGATTTGATTCAAATTG
GGGCGTACAAACGAGGATCGTCCCAAGAAATTGATGAGGCGATCGAATTTTATCCGCAAATGATGGCGTTTTGCCGCCAA
AACATCGATGAAAAAACGACGCGCGCCGAAAGCATTGATGCCCTGTTGCGCTTGCTGTCGGTTCGGTGA

Protein sequence :
MYKRFGKVSRVIGLMMESKGPKSSIGDLCYIHEQSGCRKIPAEVVGFHEQHVLLMPFSATTHIAPGCLVEATGRPLEVHV
GDALIGMVLDPLGQPLDGSPLPPGLKPVSVERDPPNPLVRPPIVEPLEVGIRVIDSLLAVGKGQRVGIFAGSGVGKSTLM
GMIARHTSADVNVIALIGERGREVREFLERDLGPDGLARSVVVVATSDQPALMRVKGAYTATAIAEYFRDQGADVMLMMD
SVTRVAMAQREIGLAVGEPPTTKGYTPSVFALLPKLLERAGTNASGTITAFYTVLVDGDDMNEPIADAVRGILDGHFVLE
RQLANKGHYPAINVLKSVSRVMPHMISAEHREAANRFRRLLSTYIDSEDLIQIGAYKRGSSQEIDEAIEFYPQMMAFCRQ
NIDEKTTRAESIDALLRLLSVR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-100 54
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-100 54
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-101 53
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 4e-102 53
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 4e-102 53
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-102 53
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-101 53
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-101 53
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-102 53
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 5e-92 53
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-99 51
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-90 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-90 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-90 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-90 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-90 49
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-84 49
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-84 49
escN CAC81862.1 EscN protein Virulence LEE II Protein 7e-85 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 8e-85 47
escN CAI43874.1 EscN protein Virulence LEE Protein 5e-85 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 7e-85 47
escN AAC31513.1 L0034 Virulence LEE Protein 4e-85 47
escN NP_290268.1 hypothetical protein Virulence LEE Protein 6e-85 47
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-85 47
ECs4568 NP_312595.1 EscN Virulence LEE Protein 6e-85 47
escN AAC38383.1 EscN Virulence LEE Protein 2e-85 47
escN AAK26715.1 EscN Virulence LEE Protein 2e-84 47
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-85 47
escN AAL57542.1 EscN Virulence LEE Protein 2e-84 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 5e-75 47
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 4e-77 47
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 9e-76 46
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-81 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 4e-77 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-77 46
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-78 46
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-73 45
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-73 45
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 8e-80 45
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 7e-80 45
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-80 45
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 3e-86 45
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-70 43
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 6e-70 43
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-70 43
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-70 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 2e-72 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 8e-72 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 2e-69 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_003253104.1 flagellum-specific ATP synthase VFG0390 Protein 3e-103 55
fliI YP_003253104.1 flagellum-specific ATP synthase VFG2144 Protein 6e-101 53
fliI YP_003253104.1 flagellum-specific ATP synthase VFG0048 Protein 4e-97 53
fliI YP_003253104.1 flagellum-specific ATP synthase VFG1254 Protein 8e-99 52
fliI YP_003253104.1 flagellum-specific ATP synthase VFG0192 Protein 3e-98 51
fliI YP_003253104.1 flagellum-specific ATP synthase VFG2499 Protein 2e-90 50
fliI YP_003253104.1 flagellum-specific ATP synthase VFG0515 Protein 2e-90 49
fliI YP_003253104.1 flagellum-specific ATP synthase VFG2331 Protein 2e-92 49
fliI YP_003253104.1 flagellum-specific ATP synthase VFG0730 Protein 9e-86 47
fliI YP_003253104.1 flagellum-specific ATP synthase VFG0812 Protein 2e-85 47
fliI YP_003253104.1 flagellum-specific ATP synthase VFG1008 Protein 3e-72 44
fliI YP_003253104.1 flagellum-specific ATP synthase VFG2459 Protein 2e-76 43
fliI YP_003253104.1 flagellum-specific ATP synthase VFG0555 Protein 2e-70 43
fliI YP_003253104.1 flagellum-specific ATP synthase VFG1778 Protein 6e-70 41