Gene Information

Name : GYMC10_4575 (GYMC10_4575)
Accession : YP_003244603.1
Strain : Paenibacillus sp. Y412MC10
Genome accession: NC_013406
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 5174162 - 5175814 bp
Length : 1653 bp
Strand : -
Note : TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase; KEGG: bcy:Bcer98_1756 2,3-dihydroxybenzoate-AMP ligase

DNA sequence : 
ATGTTGGCAGGTTGTAAGGTTTGGCCGGAGGAATTTGCCGCCCGCTACCGGGAGGCAGGCTGCTGGCAGGGCGAAACCTT
TGGAGAGCTCTTGCGTAAACGGGCTGCAGCGCATGGGGAACGGGTTGCGGTCGTGTGCGGCGACCGGCACATAAGCTACG
CCGAACTGGACAACCGGGTGGATCGATTGGCCGCGGGATTTCTTAAGCTCGGCATTAAGGCACAGGAACGGGTTATCGTT
CAGCTGCCGAACATCGCTTCATTTTTCGATACGATATTCGCGCTGTTCCGCATAGGGGCGCTTCCCGTCTTCGCTCTGCC
CGTTCACCGCCGGAACGAAATCGTATATCTATGCGGCTTTAGCGAAGCCGCCGCGTATATCATTCCCGATATGGAATCGG
GCTTCGACTATCGCGACCTGGCGGCTCAGGTTCAGGCAGCGGTCCCTTCCCTCCGCCATGTCATCGTAGCCGGCGACCCG
GGTCCGGGGGCGTTCGTATCCCTGGACGATTTGTACCTCGATGATTTGGAGTGCTCTTCGCACGGATCAGACGCGGAAGG
CCCTAATCCCGGTGATGTCGCGTTTCTCCAGCTGTCGGGCGGGACGACCGGACTGCCGAAGCTCATTCCGCGCACGCATG
ACGACTATATCTACAGCTTGCGCATGAGCGCCGAAATCTGCTCGCTGGATAAGGATAGCGTTTATTTGGCGGTACTTCCC
GTCGCGCATAATTTCCCGCTCAGCTCGCCGGGCGTGCTGGGGACGCTGTATGCCGGAGGCAGGGTTGTTCTTGCCCGGTC
AGCGAGTCCGGATGCGGCATTTCCGCTGATCGCCTCCGAAGGCGTCACGATAACGGCGGTCGTTCCGCCCCTGGCCCTGA
TTTGGCTTGAATCGGCACCATCCCGTTCCGAAGACCTCTCCAATCTGCAGGTGCTGCAGGTAGGCGGGGCCAAATTCAGT
GCGGAGGCGGCCAAACGCGTGCAAACGGTGCTGGGATGCAAGCTGCAGCAAGTGTACGGGATGGCGGAAGGCTTGGTGAA
CTATACCCGGCTGGATGATCCCGAAGACATAACGGTGCATACGCAGGGGCGTCCCATGTCTCCTTACGATGAAGTGAGGC
TGGTCGATGAGGATGATGTCGAGGTAGCCCCCGGCGAAGTCGGGGAATTGCTGACACGGGGGCCTTATACGATTCGCGGC
TACTATAAAGCGGACAAGCATAACGAGAAGGCTTTTACCACGGACGGCTTCTATCGCACGGGTGACCTTGCCAGCATGAC
GCCTTCAGGTTATCTGGTGATCCAAGGCCGTTCCAAGGATCAAATTAACCGCGGGGGCGACAAGGTTGCGGCCGAAGAGG
TAGAGAACCATCTGCTTGCCCATCCCAGCGTGCACGATGCTGCCGTGGTCGCGATGCCGGACGAGTATCTGGGCGAGCGG
ACCTGCGCCTTCGTTATCGTTCATCATGGCGCGGTTAGCGGCAATGAGCTGAAGGCGTTTCTCAGAAATAGAGGGCTCGC
TGCGTACAAAATCCCGGATCGGGTGGAATTTATGGATTCCTTCCCCCACACGGGTGTCGGCAAAGTAAGCAAAAAGCAGC
TGCGGGAGCTCGCGGCTCAGAATCAGGACGCACGGCTGAAACTTTCCAAATAA

Protein sequence : 
MLAGCKVWPEEFAARYREAGCWQGETFGELLRKRAAAHGERVAVVCGDRHISYAELDNRVDRLAAGFLKLGIKAQERVIV
QLPNIASFFDTIFALFRIGALPVFALPVHRRNEIVYLCGFSEAAAYIIPDMESGFDYRDLAAQVQAAVPSLRHVIVAGDP
GPGAFVSLDDLYLDDLECSSHGSDAEGPNPGDVAFLQLSGGTTGLPKLIPRTHDDYIYSLRMSAEICSLDKDSVYLAVLP
VAHNFPLSSPGVLGTLYAGGRVVLARSASPDAAFPLIASEGVTITAVVPPLALIWLESAPSRSEDLSNLQVLQVGGAKFS
AEAAKRVQTVLGCKLQQVYGMAEGLVNYTRLDDPEDITVHTQGRPMSPYDEVRLVDEDDVEVAPGEVGELLTRGPYTIRG
YYKADKHNEKAFTTDGFYRTGDLASMTPSGYLVIQGRSKDQINRGGDKVAAEEVENHLLAHPSVHDAAVVAMPDEYLGER
TCAFVIVHHGAVSGNELKAFLRNRGLAAYKIPDRVEFMDSFPHTGVGKVSKKQLRELAAQNQDARLKLSK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 7e-113 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-113 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-113 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 9e-114 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 9e-113 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-113 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 7e-113 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-113 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-113 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
GYMC10_4575 YP_003244603.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 4e-134 54
GYMC10_4575 YP_003244603.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 2e-111 48
GYMC10_4575 YP_003244603.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 6e-112 48