Gene Information

Name : fliI (CD196_0267)
Accession : YP_003213316.1
Strain : Clostridium difficile CD196
Genome accession: NC_013315
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 315523 - 316842 bp
Length : 1320 bp
Strand : +
Note : -

DNA sequence :
ATGTATAAAACTGATATATCTATAGATTTTGACAAAATAAATAAGAAAATAGAAAATTTAGAACTCACTATATCAGAAGG
TAGAGTAAAGAAGATAATAGGACTTACTGTAGAAGTTGAAGGAATAAAAGCTTTTGTTGGGGAGTTATGTGTTATATACA
ATCAAGTCAATAAACCAGTGAATTGTGAAGTAGTGGGTTTTAAAGATAAAGAAGTCATATTAATGGCGTTGGGAGAGCTA
ACATTGATAGCTCCTGGTTGTAAAGTTATTTCTAAGGGAATACCTTTAAGTGTTATGTGTAGCGATAATTTACTAGGTAA
AGTACTTGATGGTCTTGGAAATCCTATAGACAACTCAGATGCTGTTTTAGGAGATAGATACAACTTGAACAATGAACCTC
CTGACCCAATGAAGAGAAAAAAGATACGAAATATAATGGAAACAGGTGTAAGAGCAATAGATGCATTTACTACTTGTGGT
GAAGGACAGAGAATAGGGATATTTGCAGGAAGTGGAGTAGGTAAGAGTACGACTCTAGGTATGATTGCTAGGAATGCAAA
AGCAGATGTAAATGTAATTGCCCTTATTGGAGAAAGAGGAAGAGAGGTACTAGATTTTATTGATAAAGATTTAGGTGAAG
AGGGAATGAAAAAATCTGTTGTTGTATGTGCAACATCAGATAAAGCTCCTTTGGTTAGGCTAAAAGGTGCTCTTACTGCT
ACTGCAATAGCAGAGTATTTTAGAGACCAGGGAAAAAAAGTAATACTTATGATGGATTCAGTAACTAGATTTGCTATGGC
ACAAAGAGAAGTTGGTATAGCAATAGGAGAACCACCAGCTCAAAAAGGATATACACCATCTGTATTTGCTATTTTACCAA
AACTTATGGAGAGAACAGGGACATCAGATAAAGGTTCTATAACTGCATTTTATACAGTATTAGTTGATGGAGATGACTTT
AATGAGCCAATAGCAGATACAACAAGAGGGATACTTGATGGTCATATAGTTTTATCTAGAGACTTGGCAAATAAGAATCA
TTATCCTTCTATAGATGTATTGAACAGTTTAAGCAGACTTATGAATGAGATTGCTTCAAAAGAAGACATAAAAATAGCAT
CTTTTGCGAGAGATATGCTTGCTGAGTATAGAGAAGCAGAAGATTTGATAAATATAGGTGCATATGCTTCAGGTACAAAC
AAAAAAATAGATGAAGCAATCTACTATCATGAGCATATTATTAATTTTTTAAAACAAGGAATAAATGAAAAGAGTTCTTT
TAATGAAACTATAAGTAGTTTAAGAAGAATCTTTGAATAA

Protein sequence :
MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKAFVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGEL
TLIAPGCKVISKGIPLSVMCSDNLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKKSVVVCATSDKAPLVRLKGALTA
TAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIGEPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDF
NEPIADTTRGILDGHIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINIGAYASGTN
KKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 5e-83 55
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-100 53
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-80 49
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-80 49
escN AAC38383.1 EscN Virulence LEE Protein 3e-80 49
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-80 49
escN AAC31513.1 L0034 Virulence LEE Protein 1e-80 49
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-80 49
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-80 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-96 48
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-96 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-97 48
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-97 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-97 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-97 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-96 48
unnamed AAL06369.1 EscN Virulence LEE Protein 4e-79 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-96 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-96 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-79 46
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-79 46
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-80 46
escN AAL57542.1 EscN Virulence LEE Protein 5e-80 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 4e-80 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 3e-80 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 4e-80 45
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-80 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-80 45
escN AAK26715.1 EscN Virulence LEE Protein 5e-80 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 6e-80 45
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-80 45
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 7e-71 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-71 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-73 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-73 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-78 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-72 43
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-81 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-67 43
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-75 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-75 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-76 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-69 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-69 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-65 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-65 41
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-65 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-65 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_003213316.1 flagellum-specific ATP synthase VFG2144 Protein 6e-114 54
fliI YP_003213316.1 flagellum-specific ATP synthase VFG0390 Protein 7e-100 53
fliI YP_003213316.1 flagellum-specific ATP synthase VFG0192 Protein 1e-99 51
fliI YP_003213316.1 flagellum-specific ATP synthase VFG0048 Protein 1e-97 49
fliI YP_003213316.1 flagellum-specific ATP synthase VFG0812 Protein 8e-81 49
fliI YP_003213316.1 flagellum-specific ATP synthase VFG0730 Protein 1e-80 49
fliI YP_003213316.1 flagellum-specific ATP synthase VFG1254 Protein 1e-93 48
fliI YP_003213316.1 flagellum-specific ATP synthase VFG2331 Protein 2e-85 47
fliI YP_003213316.1 flagellum-specific ATP synthase VFG0515 Protein 1e-79 46
fliI YP_003213316.1 flagellum-specific ATP synthase VFG2499 Protein 3e-87 46
fliI YP_003213316.1 flagellum-specific ATP synthase VFG0555 Protein 1e-65 41