Gene Information

Name : pykF (CD196_3170)
Accession : YP_003216184.1
Strain : Clostridium difficile CD196
Genome accession: NC_013315
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 3753623 - 3755383 bp
Length : 1761 bp
Strand : -
Note : -

DNA sequence :
ATGTTAAATAATGTAAAGAAGACAAAAATTGTTTGTACACTAGGTCCAGCATCACAAAGTGAGGAAATTTTAACTCAACT
TATGCAAAATGGTCTAAATGTGTGTAGATTTAACTTTTCTCACGGTTCGCATGAAGAACATAAAGAAAGAATTGATATGG
CAAAGAAAGTTAGAGAAAAATTAAATAAGCCAGTTGCTATATTATTAGATACTAAAGGTCCAGAAATTAGAACAGGTAAC
TTTGAAGACCCAGAGGTATTTTTAGAAGAAGGGCAAAAGTTCACTATAACTATGAAAGATATTATGGGGACTAAAGAAAT
GTGTACAGTAAGCTATAAAGGTCTTGCTGAAGACGTTAAATCAGGAGATTCTATACTAATAGATGATGGTTTAGTTGGCT
TAAGAGTAAAAGAAATAAATGGAGAAGATATAGTTTGTGTTGTTGAAAATTCAGGAATAGTTAAAAATCATAAAGGAGTA
AATGTACCTGGTGTTAAGATAAATTTACCAGCAATAACTCCTAAAGATATAAGTGATATAGAGTTTGGAATAAGTCAAGG
AATAGATTATATAGCTGCTTCTTTTGTAAGAAAGGCATCTGATGTACTAGCAATAAGAGAAGTATTAGAGAACAATAATG
CAACTGATATACAAATAATATCTAAAATAGAAAACCAAGAAGGTGTAGAGAACCTAGATGAAATATTAAAAGTTTCTGAT
GGTATAATGGTGGCTAGAGGTGACTTAGGTGTTGAGATACCAACTGAAGAGATGCCAATAGTTCAAAAAATGATGATAAA
AAAATGTAATGAATTAGCAAAGCCAGTTGTTACTGCCACTCAAATGCTTGATTCTATGATAAGAAATCCAAGACCAACAA
GAGCAGAAGTTACAGACGTTGCCAATGCTATATATGATGGAACAGATGCGATAATGTTATCTGGAGAAACTGCTGCTGGA
AAATATCCTGTAGAGGCAGTAAAAATGATGGCTACAATAGCAAAAAGAACAGAAGAGACTTTAGATTATGATAGATTACT
AAAGGAAAATGGTACTAACAATGTTACTGTAACAGATGCAATAAGCCATGCAACTTGTACAACAGCAGTAGATTTAAATG
CATCAGCTATAATAACATCTACTTCTTCAGGATATACTGCTAGAATGGTATCTAAATTTAGACCAAAATCTCCAATAATA
GCTACTACAAATAATGAAAAGACTATGAATAAATTAGCTCTTACATGGGGAGTATATCCAGTTAAGTCTTCTGTTGCAGG
AAATACAGATGAAGTTATAGAAAAGGCAATTGAGGCAGCTAGACAAGCTGATTATATAGATAATGGGGAATTAGTAGTTA
TAACTGCTGGAGTACCAGTAGGAGTAAGTGGAACTACTAATTTAATAAAGGTTCATGTAATAAGCGAAGAAATCGTGCAA
GGTATAGGTGTAGGTACTCAAACGGTAGAAGGTAAAGTTCGTATAATAAAGAATGGAAATGCTTGTGTAGACTTTAATGA
AGGCGACATACTTGTTTCTACTGCTACTGATGCTGAAATGAACAATTATATAGAAAAAGCAGGAGCTGTAGTAACTGAGA
ATGGTGGAATGACATCACATACAGCTATTGTAGGTATAAATTTAGATATACCAGTTATTGTGTCTGCAACTGATATAACA
AATCTAGTAAAAGATGGAGAAGTTGTAACTGTGGATGCATCAAGAGGCGTGGTATATAGAGGTTCAACTAGAGTTTTATA
A

Protein sequence :
MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSHEEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGN
FEDPEVFLEEGQKFTITMKDIMGTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQIISKIENQEGVENLDEILKVSD
GIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPVVTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAG
KYPVEAVKMMATIAKRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSKFRPKSPII
ATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGELVVITAGVPVGVSGTTNLIKVHVISEEIVQ
GIGVGTQTVEGKVRIIKNGNACVDFNEGDILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDIT
NLVKDGEVVTVDASRGVVYRGSTRVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-103 51
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-103 51
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-103 51
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-103 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF YP_003216184.1 pyruvate kinase VFG0479 Protein 4e-104 51