Gene Information

Name : Bfae_14930 (Bfae_14930)
Accession : YP_003154914.1
Strain : Brachybacterium faecium DSM 4810
Genome accession: NC_013172
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1680941 - 1682410 bp
Length : 1470 bp
Strand : +
Note : PFAM: Pyruvate kinase, barrel domain; Pyruvate kinase, alpha/beta domain; TIGRFAM: pyruvate kinase

DNA sequence :
ATGCGCAAAGCGAAGATCGTCTGCACTCTCGGTCCCGCGACCAACACGTACGAGCAGATCCGCACGCTGATCGAGGCCGG
GATGAACGTCGCCCGGATGAACTTCAGCCACGGCACGCACGACGATCACGCGCAGGTGTACGCGAACATCCGGCGCGCGT
CCGAGGATCTGGGCAAGAACGTCGCCGTCCTCGTGGACCTGCAGGGGCCGAAGATCCGCCTGGGCACCTTCGCGGACGGC
CCGCACCAGCTCGAGGTCGGCGAGACCTTCACCATCACGACCGATGACGTCGACGGCACCCGCGAGCGGGTCTCGACCAC
CTTCAAGGGCCTGCCGGGCGACTGCCGCCCCGGTGACGTGCTGCTCATCGACGACGGCAAGGTCTCGGTGCGCGTCACCG
AGGTCACCGACACCGAGGTCGTGACGGTCGTCGAGGTCCCCGGCCCGGTCTCGAACAACAAGGGCATCAACCTGCCGGGC
GTGGCCGTCTCCGTGCCCGCCATGAGCGAGAAGGACATCGCCGATCTCCGCTTCGGCCTGGGCCTGGGCGCGGACCTGGT
GGCGCTCTCCTTCGTGCGCGACGCGCACGACATGGACGACGTCCTGCGCATCATGCAGGAGGAGGACCGCCGCGTCCCCG
TGGTCGCCAAGATCGAGAAGCCGCAGGCCGTGCGGGCGCTGCGCTCCATCGTCGGCGCCTTCGACGGCATCATGGTCGCC
CGCGGCGACCTCGGCGTCGAGCTGCCGCTCGAGCAGGTCCCGCTGGTCCAGAAGCGCGCCATCGAGCTCGCCCGCCGCAA
CGCGAAGCCCGTGATCGTGGCCACGCAGGTGCTCGAGTCGATGATCGAGAGCCCGCGCCCCACCCGCGCGGAGGCCTCGG
ACTGCGCCAACGCGATCCTCGACGGCGCCGACGCGGTCATGCTCTCGGGCGAGACCAGCGTGGGCAAGTATCCCTTCGAG
GCCGTGCGCACGATGGCGCGCATCGTCACGAACACCGAGGAGAACGGCGCGGACCGGATCCTCCCGCTGGGCACCATCCC
GCACACCCGCGGCGGTGCGATCACCCGCGCTGCCGCCGAGATCGGCGATCAGCTCTCCGCGCAGTACCTCGTGACGTTCA
CCGAGTCCGGTGACACCGCGCGCCGCCTGTCCCGCCTGCGGCCCACGATCCCGCTGCTGGCCATGACGCCGTACCCCGAG
GTGGCTCGCCAGCTGTCGCTGACCTGGGGCATCGAGGCGCACCTGGTGCCGATGCAGAAGGACTCCGACACGATGGTCGG
CGTGGTCGACGAGCTGCTGCGCGAGCAGAAGGGCCTGCAGAAGAACGACCTGGTCATCATCGCGGCCGGCTCCCCTCCCG
GGGTGCACGGCTCGACCAACACGCTGCGCGTGCACCGCATCGGCGACCTCGACGGCACCGAGTCGGCACGCATCGAGGCG
GACCGCATCGCCGCCGGCCCGCAGGGCTGA

Protein sequence :
MRKAKIVCTLGPATNTYEQIRTLIEAGMNVARMNFSHGTHDDHAQVYANIRRASEDLGKNVAVLVDLQGPKIRLGTFADG
PHQLEVGETFTITTDDVDGTRERVSTTFKGLPGDCRPGDVLLIDDGKVSVRVTEVTDTEVVTVVEVPGPVSNNKGINLPG
VAVSVPAMSEKDIADLRFGLGLGADLVALSFVRDAHDMDDVLRIMQEEDRRVPVVAKIEKPQAVRALRSIVGAFDGIMVA
RGDLGVELPLEQVPLVQKRAIELARRNAKPVIVATQVLESMIESPRPTRAEASDCANAILDGADAVMLSGETSVGKYPFE
AVRTMARIVTNTEENGADRILPLGTIPHTRGGAITRAAAEIGDQLSAQYLVTFTESGDTARRLSRLRPTIPLLAMTPYPE
VARQLSLTWGIEAHLVPMQKDSDTMVGVVDELLREQKGLQKNDLVIIAAGSPPGVHGSTNTLRVHRIGDLDGTESARIEA
DRIAAGPQG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-69 42
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-69 42
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-69 42
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-69 42
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 1e-59 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Bfae_14930 YP_003154914.1 pyruvate kinase VFG0479 Protein 1e-69 42