Gene Information

Name : lysS (Coch_1485)
Accession : YP_003141592.1
Strain : Capnocytophaga ochracea DSM 7271
Genome accession: NC_013162
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 1763032 - 1764720 bp
Length : 1689 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTTATCAGAACAAGAAATCATTCGCAGAGAAAAACTCTCTAAATTGAAAGAATTGGGGATAAACCCTTATCCCGCTCC
ATTATATCCGGTTGATACTACCTCTAAGGACGTAGCCAACCATTATGAAGAAGGTAAACAAGTGGTTGTAGCCGGTAGAT
TAATGTCGTTGCGTGTACAAGGAAAAGCCTCGTTTGCTACTTTGCAAGATAGTGAAGGCACGATGCAATTGTACTTCAAC
CGCGATGAGATGTGCCCTACCGATGATAAAACGCTTTACAACGAGGTGTTTAAAAAATTATTGGATTTAGGTGACTTTAT
AGGTGCTGAAGGAACACTCTTCATCACTAAAATGGGTGAGAAAACTGTGTTGGTAAAGAACTTTACGCTACTTTCAAAAG
TATTACGTCCGCTACCTATGCCTAAGGTAGATGCCGATGGTAAAGTACACGATGCCTTCACCGATCCTGAACAACGCTAC
CGTATGCGCTATGTAGATTTGGTAGTAAATCCACAAGTGAAAGAAGTGTTTATCAAGCGCACTAAACTCTTCAATGCTAT
GCGTCAGTTTTTTAACGATGCAGGTTATTTGGAAGTAGAGACACCTATACTACAAGCTATACCAGGGGGAGCTAGTGCAC
GTCCGTTTATCACCCATCACAATGCGTTGGACATTCCGCTGTATATGCGTATTGCCAATGAACTTTACTTAAAACGTCTT
ATTGTAGGAGGTTTTGACGGAGTGTATGAATTTTCTAAGAACTTCCGCAACGAGGGTATGGATAGAACTCACAACCCTGA
GTTTACGGCTATGGAAATTTATGTGGCTTACAAAGATTATCACTGGATGATGGACTTCACTGAACGCTTGATTGAGCATT
GCGCTATGGCGGTTAATGGTACTACTAAGGCGACCTTTGGTAAACATACAGTAGATTTCAAAGCTCCTTATCCACGCCTT
ACGATGACTGATGCTATTAAACAGTTCACAGGCTTTGACATTACTGCGAAGACAGAAGACGAATTGAGAGCCTTTGCACA
GTCTATCGGTATTGCAGTAGACGAGGCGATGGGCAAGGGCAAATTGATAGATGAAATATTTGGTGAGAAATGCGAAGGCA
ACTTCATTCAGCCTACTTTCATCACTGATTACCCCAAAGAGATGTCGCCACTGACCAAAGAACACCGCGACAACCCCGAC
CTTACTGAACGTTTTGAGTTAATGGTATGCGGTAAGGAGATTGCCAATGCTTATTCAGAATTAAACGACCCTATTGACCA
ACGTGAGCGCTTTGAAGAACAATTGCGACTCTCAGAAAAAGGCGACGACGAAGCGATGTTTATTGACCAAGATTTTTTGC
GTGCTCTTGAATACGGTATGCCACCTACCTCAGGTTTGGGTATCGGTATGGACAGACTCGTAATGTTCCTTACCAACAAT
GAGAGTATACAAGAAGTATTGTTCTTCCCACAAATGCGCCCCGAGAAGAAAGAAGTAGAGCTTTCGGAAGACGAGAAAGT
AGTGTTTGAGCTTTTAAAAACCTCAGAGAGACTGCTTTTAGATGCTCTCAAAACACAAACAGCATTTTCTAACAAGAAAT
GGGATAAAGCCATTAAAGGTCTTACTAGTAAGAAAGTAGCAAAGGTAGTAAAAGAAGGAGAAGAACTGTTTGTGGAATTA
GCAAATTAA

Protein sequence :
MLSEQEIIRREKLSKLKELGINPYPAPLYPVDTTSKDVANHYEEGKQVVVAGRLMSLRVQGKASFATLQDSEGTMQLYFN
RDEMCPTDDKTLYNEVFKKLLDLGDFIGAEGTLFITKMGEKTVLVKNFTLLSKVLRPLPMPKVDADGKVHDAFTDPEQRY
RMRYVDLVVNPQVKEVFIKRTKLFNAMRQFFNDAGYLEVETPILQAIPGGASARPFITHHNALDIPLYMRIANELYLKRL
IVGGFDGVYEFSKNFRNEGMDRTHNPEFTAMEIYVAYKDYHWMMDFTERLIEHCAMAVNGTTKATFGKHTVDFKAPYPRL
TMTDAIKQFTGFDITAKTEDELRAFAQSIGIAVDEAMGKGKLIDEIFGEKCEGNFIQPTFITDYPKEMSPLTKEHRDNPD
LTERFELMVCGKEIANAYSELNDPIDQRERFEEQLRLSEKGDDEAMFIDQDFLRALEYGMPPTSGLGIGMDRLVMFLTNN
ESIQEVLFFPQMRPEKKEVELSEDEKVVFELLKTSERLLLDALKTQTAFSNKKWDKAIKGLTSKKVAKVVKEGEELFVEL
AN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-99 45
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 5e-83 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_003141592.1 lysyl-tRNA synthetase VFG1668 Protein 3e-99 45