Gene Information

Name : Amir_0702 (Amir_0702)
Accession : YP_003098511.1
Strain : Actinosynnema mirum DSM 43827
Genome accession: NC_013093
Putative virulence/resistance : Resistance
Product : cystathionine gamma-synthase
Function : -
COG functional category : E : Amino acid transport and metabolism
COG ID : COG0626
EC number : 2.5.1.48
Position : 780021 - 781172 bp
Length : 1152 bp
Strand : +
Note : PFAM: Cys/Met metabolism pyridoxal-phosphate- dependent protein; aromatic amino acid beta-eliminating lyase/threonine aldolase; KEGG: afw:Anae109_3845 cystathionine gamma-lyase

DNA sequence :
ATGACTGACAGCGGCCCACTCGGCTTCGCAACGCGGGCGATCCACACCGGACAGGAGCCGGACCCCACCACCGGCGCGGT
CATCCCGCCGCTCTACGCCACCTCCACCTACGCCCAGGACGGCGTCGGCGGGCTGCGCAAGGGCTTCGAGTACTCGCGCA
CCGGCAACCCAACCCGCACCGCGCTGGAGCAGTGCCTGGCCGCGCTGGAGGGCGGCCGGTTCGGGCGGGCGTTCGCCAGC
GGCATGGCCGCCACCGACACCGCGCTGCGGTCGCTGCTGCGCCCCGGTGACCACCTGGTCATCCCGAACGACACCTACGG
CGGCACGTTCCGCCTCATCGACAAGGTGCTGTCCCAGTGGGGCGTCGAGCACACCCCCGTCGCGATCTCCGACGTGGACG
CCGTGCGCGCCGCGATCCGCCCGAACACCAAGCTGGTGTGGGTCGAGACGCCGACCAACCCGCTGCTCACCATCGCGGAC
ATCGCGCTGCTGGCCGACGCCACCCGCGACGCCTCGGTGACGCTGGTCGTGGACAACACGTTCGCCTCGCCCTACCTGCA
GAACCCCCTCGAACTGGGCGCGGACGTGGTCATGCACTCCACGACCAAGTACCTGGGCGGCCACTCCGACCTCATCGGCG
GCGCGCTGGTCACCTCCGACGAGGAGCTCGACACCAAGTTCGCGTTCCTGCAGAACGGCTCCGGCGCGGTGCCCAGCCCG
TTCGACGCGTGGCTGACCCTGCGCAGCCTCAAGACCCTCCAGGTCCGCATGGAGAAGCACTCGGACAACGCCGAGGCGGT
CGCGCAGGCCCTGTCCGCGCACCCGAAGGTGGAGCGCGTGCTCTACCCCGGCCTGCCCGAGCACCCCGGCCACGAGACGG
CGGCCAAGCAGATGCGCCGGTTCGGCGGCATGGTGTCGTTCCTGGTCAAGGGCGGCGAGCAGGCCGCGCTCGACGTGTGC
GCGCGCACGAACGTGTTCACGCTGGCCGAGTCGCTGGGCGGCGTCGAGTCGCTGATCGAGCACCCCGGCCGCATGACGCA
CGCCAGCGTCGCGGGCACCGAGCTGGAGGTCCCGGCCTCGCTCGTGCGGATCTCGGTCGGCCTGGAGGACGCGGACGACC
TGATCGCCGACCTGTCGCAGGCCCTGGCCTGA

Protein sequence :
MTDSGPLGFATRAIHTGQEPDPTTGAVIPPLYATSTYAQDGVGGLRKGFEYSRTGNPTRTALEQCLAALEGGRFGRAFAS
GMAATDTALRSLLRPGDHLVIPNDTYGGTFRLIDKVLSQWGVEHTPVAISDVDAVRAAIRPNTKLVWVETPTNPLLTIAD
IALLADATRDASVTLVVDNTFASPYLQNPLELGADVVMHSTTKYLGGHSDLIGGALVTSDEELDTKFAFLQNGSGAVPSP
FDAWLTLRSLKTLQVRMEKHSDNAEAVAQALSAHPKVERVLYPGLPEHPGHETAAKQMRRFGGMVSFLVKGGEQAALDVC
ARTNVFTLAESLGGVESLIEHPGRMTHASVAGTELEVPASLVRISVGLEDADDLIADLSQALA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SAR0460 YP_039909.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 2e-76 46
SAS0418 YP_042545.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 3e-77 46
metB YP_281510.1 cystathionine beta-lyase Not tested Not named Protein 3e-57 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Amir_0702 YP_003098511.1 cystathionine gamma-synthase BAC0207 Protein 3e-65 45