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Name : lysS (Dfer_4009) Accession : YP_003088378.1 Strain : Dyadobacter fermentans DSM 18053 Genome accession: NC_013037 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 4858147 - 4859862 bp Length : 1716 bp Strand : - Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGCTACTGAGCGAACAGGAAATACTGCGCCGTCAAAAGCGCGAAGAACTCATGCGGATGGGCATTGAGCCTTACCCTGC CGAGGAATTTATCGTGAACGCCTACGCGGCCGACATCCTTAAAAACTACGAAAACAACAAGATCGACTATAAACACGTGA CCATGGCCGGCCGGCTGATGGGCTTCCGCATTATGGGAAGCGCCGCTTTCGCCGAGTTCCAGGACAGCACGGGACGCGTT CAACTTTACTTCCGCCGCGACGACCTCTGCCCCGGCGAAGACAAGACATTATATAATACTGTTTTCAAAAAGCTGCTCGA TATCGGTGACATGATCGCCGTTACGGGCTTTGTATTTACTACCCAAACCGGCGAAATCTCGGTTCACGTGCAGGAATTCA AGATCCTGAACAAATCGCTCCGCCCGCTGCCGGTGGTGAAGCGTGACGAGGACGGCAATGTACACGACGGATTCACCGAC CCCGAACTGCGTTACCGCCAACGTTACGTGGACCTAATCGTTAACCCCGAGGTGCGCGAGATATTTATCAAACGGGCCCG GATCATCACCACCATGCGTTCGCATTTTGATTCGAACGGCTGGCTGGAAGTGGAAACGCCGGTGCTGCAATCGATCCACG GCGGTGCGGCTGCAAGGCCTTTCACCACGCACCACAATGCGCTCGACCTGGGCTTGTACCTGCGTATCGCGACGGAGCTG CATTTGAAACGGCTCATTGTAGGCGGTTTTGAAGGCGTGTATGAGTTTGGCAAACTGTTCCGTAATGAAGGAATGGACCG CACGCACAACCCCGAGTTTACGACCGTGGAGCTCTATGTGGCTTACAAGGATTACCTCTGGATGATGAAAATGACCGAGG AGGTGCTCGAAAAGGTGGCCCTGGCCGTGAATGGCACCACCAAAGCCACTTTCAATGGCGTGGAGCTCGATTTTGCAGGC CCTTACCCAAGGCTCAGCATTACAGATGCGATCAAGCAATTTACTGGCCTGGATGTGGAGGCGCTCGACGAAGTGGCGAT CCGCGAACAATGCCGTGCATGGGGCATGGAGGTGAACGAAAGCATGGGCAAAGGCAAGCTGATCGACGAAATTTTCGGTG AGAAAGTGGAATCGAACATTATCCAGCCTACGTTCATCATCGACCACCCGGTTGAAATGTCGCCGCTGACGAAGAAACAC CGCACCAAAAAAGGCATGGTAGAGCGTTTCGAGCTGTTTGTGAATGGCCGCGAGATCGCCAATGCCTATTCTGAGCTCAA TGACCCTATCGAGCAGAAAGAGCGTTTCGAAGACCAGCTCCGACTGAAAGAGAAGGGCGACGACGAGGCCATGGAAATGG ACGACGACTTCATCCGCTCACTGGAATACGGCATGCCTCCGACTGCCGGAATCGGGATCGGCATTGACCGCCTCACGATG TTGCTGACGGACAATGTGAGCATTCAGGAAGTGATCTTCTTCCCGCAAATGCGTCCGGAGAAGAAAGCGGAAATCGCGAA AGAATCGGACTACGTAGAAGCCGGCGTGCCGGCGGTATGGGTGCCCGCATTGCAGAAAATGAATATACTGACGATCGAGC AACTGAAAGGCGCCAATGCAAACAAGATCTTTAATGACCTGGGCGGGATGCGCAAGAAGCTCAAAATAGAAGAGAAAATG CCGCAGCTGGACGATATAAAGTCCTGGGTGGAATAA Protein sequence : MLLSEQEILRRQKREELMRMGIEPYPAEEFIVNAYAADILKNYENNKIDYKHVTMAGRLMGFRIMGSAAFAEFQDSTGRV QLYFRRDDLCPGEDKTLYNTVFKKLLDIGDMIAVTGFVFTTQTGEISVHVQEFKILNKSLRPLPVVKRDEDGNVHDGFTD PELRYRQRYVDLIVNPEVREIFIKRARIITTMRSHFDSNGWLEVETPVLQSIHGGAAARPFTTHHNALDLGLYLRIATEL HLKRLIVGGFEGVYEFGKLFRNEGMDRTHNPEFTTVELYVAYKDYLWMMKMTEEVLEKVALAVNGTTKATFNGVELDFAG PYPRLSITDAIKQFTGLDVEALDEVAIREQCRAWGMEVNESMGKGKLIDEIFGEKVESNIIQPTFIIDHPVEMSPLTKKH RTKKGMVERFELFVNGREIANAYSELNDPIEQKERFEDQLRLKEKGDDEAMEMDDDFIRSLEYGMPPTAGIGIGIDRLTM LLTDNVSIQEVIFFPQMRPEKKAEIAKESDYVEAGVPAVWVPALQKMNILTIEQLKGANANKIFNDLGGMRKKLKIEEKM PQLDDIKSWVE |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-96 | 47 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_003088378.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 6e-97 | 47 |
