Gene Information

Name : entE (TERTU_4060)
Accession : YP_003075343.1
Strain : Teredinibacter turnerae T7901
Genome accession: NC_012997
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 4489354 - 4490970 bp
Length : 1617 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
GTGAGTCAACAACACCTGCCGTCCAATACTGTTGAATTCACGCCCTGGCCTAACGATATAGCGCATCAATATCGAGACAA
AGGCTACTGGTTGGATTTGCCACTCACGGAAATTCTAAGCGAGCACCGCAATTCAGACAGTATCGCGATAGTCTGCGGTG
AGCGCACCTATACCTACCGTGAACTGGACTCTGCGAGTTCTCTGCTGGCGCTTCATTTCTGTGAACATGGCCTTGGCCCG
GGTGATACGGCACTGGTGCAATTGCCCAATGTCGCCGAGTTGTATATTACTTTTTTTGCATTGCTCAAAGCGGGTATCGC
GCCAGTTAACGCCCTGTTTAATCATCAGCGGTTGGAGTTAACTGAGTATGCCCGTCAATTAGAGCCAAGCCTGTTAATTG
CATCTCGCGACCACGCATTGTTTGCCGACGACGGCTATCTAGAAGAGTTAAAGGCATTAACTAACATTCGCCTGACGCTG
TTTCACCGCGAAAATCTAACACGCGTCTTTGACAAGAACATGGAGAGCAAAACAACCAATAGTTTTCTGCCAACAAATCC
GGAACAGGTTGCTTTCTTTCAACTGTCAGGCGGAAGTACCGGTACACCTAAACTTATTCCACGTACCCACAACGATTATT
TTTACAGCGTAAGACGCAGTGTGGAAATCTGTGGGTTCTCGCCCAGCACCCGATTTTTAGTCGCCTTGCCTGCTGCGCAT
AATTTCCCACTCAGTTCACCCGGGGCACTCGGCGTATTGTTCGCGGGTGGAACCCTGGTTATGGCGAGCAATCCGGAACC
TACACGTTGTTTCGAGCTTATCGACCAGCACTCAGTTAATACCACCGCGCTGGTGCCATCGGCTGTTCTCTTATGGCTCG
CCCACGCGGCGACTGCGCGTGAAAAACTGCGCACGCTGACGTTGCTGCAAGTTGGTGGTGCTAATTTTTCAGAGTCCACT
GCACGGCGCGTGCCCGTTGAACTTGGCTGCAAATTGCAGCAGGTATTTGGCATGGCTGAAGGATTAGTGAATTACACACG
CTTGGATGACAGCGATGAAGTGATCTTTACCAGTCAGGGCCGCCCGATGAGTCCGAACGACGAAATTCGCATTATTAACA
GCGAGGGCGAGGACGTGGCACCTGGCCACACGGGCCAGCTGATTACCCGAGGCCCTTACACCATTCGCGGCTACTACAAG
ACACCGCAACACAATAGCCGTGCCTTTGATAATCAAGGGTTTTACTACTCGGGCGACCTGGTACAGCAAACGCCCGACGG
AAATATTCGTGTGGTAGGGCGTGTTAAAGACCAGATTAATCGCGGTGGCGAAAAAATTGCCGCTGAAGAAATCGAAAATC
TCTTGGTGAAACACCCACAAATTATTAACGCCGCATTAGTCGCATTGCCCGACGAGCGACTCGGCGAAAAGAGCTGCGCC
TGCCTGGTCACTCAGGACGAAAAAATCAAACCCCTTGTGGTACGTAAATTTTTACGTGCGCAAGGCGTCGCTGAATTCAA
ACTGCCAGATCACATTGCGGTGCTACCGGAATTTCCATTAACCGCGGTTGGCAAAATCAATAAAAAAGCCTTGCGCGCAC
ACATGCAGAACTTGTGA

Protein sequence :
MSQQHLPSNTVEFTPWPNDIAHQYRDKGYWLDLPLTEILSEHRNSDSIAIVCGERTYTYRELDSASSLLALHFCEHGLGP
GDTALVQLPNVAELYITFFALLKAGIAPVNALFNHQRLELTEYARQLEPSLLIASRDHALFADDGYLEELKALTNIRLTL
FHRENLTRVFDKNMESKTTNSFLPTNPEQVAFFQLSGGSTGTPKLIPRTHNDYFYSVRRSVEICGFSPSTRFLVALPAAH
NFPLSSPGALGVLFAGGTLVMASNPEPTRCFELIDQHSVNTTALVPSAVLLWLAHAATAREKLRTLTLLQVGGANFSEST
ARRVPVELGCKLQQVFGMAEGLVNYTRLDDSDEVIFTSQGRPMSPNDEIRIINSEGEDVAPGHTGQLITRGPYTIRGYYK
TPQHNSRAFDNQGFYYSGDLVQQTPDGNIRVVGRVKDQINRGGEKIAAEEIENLLVKHPQIINAALVALPDERLGEKSCA
CLVTQDEKIKPLVVRKFLRAQGVAEFKLPDHIAVLPEFPLTAVGKINKKALRAHMQNL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-84 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-84 42
ybtE CAA21394.1 - Virulence HPI Protein 4e-84 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 4e-84 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 3e-84 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-84 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 4e-84 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-84 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-84 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_003075343.1 enterobactin synthase subunit E VFG0932 Protein 2e-154 58
entE YP_003075343.1 enterobactin synthase subunit E VFG0168 Protein 6e-111 44
entE YP_003075343.1 enterobactin synthase subunit E VFG1817 Protein 1e-89 41