Gene Information

Name : Msip34_0486 (Msip34_0486)
Accession : YP_003050261.1
Strain : Methylovorus glucosetrophus SIP3-4
Genome accession: NC_012969
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 510420 - 511856 bp
Length : 1437 bp
Strand : +
Note : KEGG: mfa:Mfla_2244 pyruvate kinase; TIGRFAM: pyruvate kinase; PFAM: Pyruvate kinase barrel; Pyruvate kinase alpha/beta

DNA sequence :
ATGGAATTACGCAGAACCAAGATCGTAGCGACGCTGGGGCCGGCCTCCAGCGAGCGTGAAGTGCTGGAACGCATGCTGGA
TGCCGGGGTGGATGTGGTGCGGCTGAATTTTTCGCATGGGCAGGCGGCAGACCATATTGCCCGGGCAGAGCTGGTGCGGG
AGCTGGCGCGTGCGCGGCGGCGCTCGGTGGGCGTGCTGTGCGATTTGCAAGGCCCCAAAATCCGTATTGGCAAGTTTGCC
GATGGCAAGGTACTGCTGCGGCCAGGTGATAATTTCATTCTGGATGCGGATTGTCCCTTGGGCGATCAATCGCGCGTGGG
CCTGGATTACAAAACCCTGCCACAAGAGGTCGTCACGGGCACGGTGCTGCTGCTGGACGATGGCCGTATCGTACTCACCG
TGGATTCAGTAGAGGGTAGTGCGGTCTATTGCACAGTCAAGGTGGGTGGCCTGCTATCCAATAACAAAGGCATTAATCGC
TTGGGCGGGGGGCTGGCAGCCGAAGCGCTGACGGAAAAAGACCGCGAGGATATCAAGACCGCTGCTGCCCTGAGCGCCGA
CTATATCGCCATCTCGTTTCCGCGCAGCGGGGCGGATGTCGAGCTGGCGCGGCAATTGGTGCAGGAAGCAGGCGGCCATG
CCCATATTGTCGCCAAGATCGAACGGGCAGAAGCGATACCCGCCCTGGAAGAGATTGTGGATGCCGCCGACGTCATCATG
GTGGCGCGCGGAGATCTGGGCGTGGAAGTGGGCGATGCCGTGGTGCCCGGCTTGCAGAAACGCATGATCCGGCTGGCGCG
CGCCAAGAACAAGCTGGTGATTACCGCCACGCAGATGATGGAATCCATGATACTCAACCCCATCCCGACCCGTGCCGAAG
TATCGGATGTGGCGAATGCCGTGCTGGATGGCACGGATGCGGTCATGCTGTCGGCGGAAACAGCGGCCGGGCAGTTTCCG
GTAGAAGCGGTGGCAGCCATGCACCGCGTATGCAAGGAAGCAGAAAAAGAATATTACGATGACAGCATGCGCCGACTGGG
CCGCGAGACCTTTGACGAAATCGACGAAGCGATTGCCATGGCCACCAGTTACAGCGCGCGGCATTTCCAGGTCAAGGCGA
TAGCGGCGCTTACCCAGTCTGGCAAATCGGTCATCTGGCTATCGCGTGCCAGCACCCATGTGCCTATCTACGCGCTGAGC
CCTGAGCAGAATACGCGCCGCAAGCTCACGCTGTGCCGTGGGGTGTATCCTTATCGCATGGATATCGCCACGCATGATAT
CGACCGTATTCTGTTTGATGCGGAAGAGGAGCTGGTCTCGCGTGGTGTGGTGACCAATGGCGATTATATTTTGCTGACCT
TTGGCCAGCCTATCGGCAAGGCTGGCAGCACCAACACCATGAAACTTGTCCAAGTAGGGGAACATCGCCAAAGTTAG

Protein sequence :
MELRRTKIVATLGPASSEREVLERMLDAGVDVVRLNFSHGQAADHIARAELVRELARARRRSVGVLCDLQGPKIRIGKFA
DGKVLLRPGDNFILDADCPLGDQSRVGLDYKTLPQEVVTGTVLLLDDGRIVLTVDSVEGSAVYCTVKVGGLLSNNKGINR
LGGGLAAEALTEKDREDIKTAAALSADYIAISFPRSGADVELARQLVQEAGGHAHIVAKIERAEAIPALEEIVDAADVIM
VARGDLGVEVGDAVVPGLQKRMIRLARAKNKLVITATQMMESMILNPIPTRAEVSDVANAVLDGTDAVMLSAETAAGQFP
VEAVAAMHRVCKEAEKEYYDDSMRRLGRETFDEIDEAIAMATSYSARHFQVKAIAALTQSGKSVIWLSRASTHVPIYALS
PEQNTRRKLTLCRGVYPYRMDIATHDIDRILFDAEEELVSRGVVTNGDYILLTFGQPIGKAGSTNTMKLVQVGEHRQS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 6e-85 49
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-60 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-60 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-60 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Msip34_0486 YP_003050261.1 pyruvate kinase VFG0479 Protein 1e-60 41