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Name : lysS (ECBD_0847) Accession : YP_003035100.1 Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG Genome accession: NC_012947 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 873870 - 875387 bp Length : 1518 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCTGAACAACACGCACAGGGCGCTGACGCGGTAGTCGATCTTAACAATGAACTGAAAACGCGTCGTGAGAAGCTGGC GAACCTGCGTGAGCAGGGAATTGCCTTCCCGAACGATTTCCGTCGCGATCATACCTCTGACCAATTGCACGCAGAATTCG ACGGTAAAGAGAACGAAGAACTGGAAGCGCTGAACATCGAAGTCGCCGTTGCTGGCCGCATGATGACCCGCCGTATTATG GGTAAAGCCTCTTTTGTTACCCTGCAGGACGTTGGCGGTCGTATTCAGCTGTACGTTGCCCGTGACGATCTTCCGGAAGG CGTTTACAACGAGCAGTTCAAAAAATGGGATCTCGGCGACATCCTCGGCGCGAAAGGTAAACTGTTCAAAACCAAAACCG GCGAACTGTCTATCCACTGCACCGAGCTGCGTCTGCTGACCAAAGCACTGCGTCCGCTGCCGGACAAATTCCACGGCTTG CAGGATCAGGAAGCGCGCTATCGTCAGCGTTATCTGGATCTCATCTCTAACGATGAATCCCGCAACACCTTTAAAGTGCG TTCGCAGATCCTCTCTGGCATTCGCCAGTTCATGGTAAATCGCGGCTTTATGGAAGTTGAAACGCCGATGATGCAGGTGA TCCCAGGCGGTGCGGCTGCGCGTCCGTTTATCACCCATCATAACGCGCTGGATCTTGACATGTACCTGCGTATCGCGCCG GAACTGTACCTCAAGCGTCTGGTGGTCGGTGGCTTCGAGCGTGTATTCGAAATCAACCGTAACTTCCGTAACGAAGGTAT TTCCGTACGTCATAACCCAGAGTTCACCATGATGGAACTCTATATGGCTTACGCAGATTACAAAGATCTGATCGAGCTGA CCGAATCGCTGTTCCGTACTCTGGCACAGGATATTCTCGGTAAGACGGAAGTGACCTACGGCGACGTGACGCTGGACTTC GGTAAACCGTTCGAAAAACTGACCATGCGTGAAGCGATCAAGAAATATCGCCCGGAAACCGACATGGCGGATCTGGACAA CTTCGACTCTGCAAAAGCGATTGCTGAATCTATCGGCATCCACGTTGAGAAGAGCTGGGGTCTGGGCCGTATCGTTACCG AGATCTTCGAAGAAGTGGCAGAAGCACATCTGATTCAGCCGACCTTCATTACTGAATATCCGGCAGAAGTTTCTCCGCTG GCGCGTCGTAACGACGTTAACCCGGAAATCACAGACCGCTTTGAGTTCTTCATTGGTGGTCGTGAAATCGGTAACGGCTT TAGCGAGCTGAATGACGCGGAAGATCAGGCGCAGCGCTTCCTGGATCAGGTTGCCGCGAAAGACGCAGGTGACGACGAAG CGATGTTCTACGACGAAGATTACGTCACCGCACTGGAACACGGCTTACCGCCGACAGCAGGTCTGGGAATTGGTATCGAC CGTATGGTAATGCTGTTCACCAACAGCCATACCATCCGCGACGTTATTCTGTTCCCGGCGATGCGTCCAGTAAAATAA Protein sequence : MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDHTSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIM GKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGAAARPFITHHNALDLDMYLRIAP ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDF GKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPL ARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID RMVMLFTNSHTIRDVILFPAMRPVK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 7e-169 | 69 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 5e-91 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_003035100.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 4e-169 | 69 |
