Gene Information

Name : ECBD_0525 (ECBD_0525)
Accession : YP_003034780.1
Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG
Genome accession: NC_012947
Putative virulence/resistance : Virulence
Product : N-acetylmannosamine kinase
Function : -
COG functional category : K : Transcription
COG ID : COG1940
EC number : -
Position : 542291 - 543166 bp
Length : 876 bp
Strand : +
Note : catalyzes the phosphorylation of the N-acetylmannosamine (ManNAc) liberated from N-acetyl-neuraminic acid by the nanA protein

DNA sequence : 
ATGACCACACTGGCGATTGATATCGGCGGTACTAAACTTGCCGCCGCGCTGATTGGCGCTGACGGGCAGATCCGCGATCG
TCGTGAACTTCCTACGCCAGCCAGCCAGACACCAGAAGCCTTGCGTGATGCCTTATCCGCATTAGTCTCTCCGTTGCAAG
CTCATGCGCAGCGGGTTGCCATCGCTTCAACCGGAATTATTCGCGACGGCAGTTTGCTGGCGCTGAATCCGCATAATCTC
GGTGGATTGCTACACTTTCCGTTAGTCAAAACGCTGGAACAACTTACCAATTTGCCGACCATTGCCATTAACGACGCGCA
GGCCGCAGCATGGGCAGAGTATCAGGCGCTGGAAGGCGATATAACCGATATGGTCTTTATCACCGTTTCCACTGGCGTCG
GCGGCGGTGTAGTGAGCGGCGGCAAACTGCTTACCGGCCCTGGCGGTCTGGCGGGGCACATCGGGCATACGCTTGCCGAT
CCACACGGCCCAGTCTGCGGCTGTGGACGCACAGGTTGCGTGGAAGCAATTGCTTCTGGTCGCGGCATTGCGGCGGCAGC
GCAGGGCGAGCTGGCTGGCGCGGATGCGAGAACTATTTTCACACGCGCCGGGCAGGGCGATGAGCAGGCGCAGCAACTGA
TTCATCGCTCCGCACGTACGCTTGCAAGGCTGATCGCTGATATTAAAGCCACAACTGATTGCCAGTGCGTGGTGGTTGGT
GGCAGCGTTGGTCTGGCAGAAGGGTATCTGGCGTTGGTGGAAATGTATCTGGCGCAGGAGCCAGCGGCATTTCATGTTGA
TTTACTGGCGGCGCATTACCGCCATGATGCAGGTTTACTTGGGGCTGCGCTGTTGGCCCAGGGAGAAAAATTATGA

Protein sequence : 
MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDALSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNL
GGLLHFPLVKTLEQLTNLPTIAINDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSARTLARLIADIKATTDCQCVVVG
GSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHDAGLLGAALLAQGEKL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
nanK YP_002414055.1 N-acetylmannosamine kinase Not tested Not named Protein 2e-38 54
VC1782 NP_231417.1 N-acetylmannosamine kinase Not tested VPI-2 Protein 6e-27 41
VC0395_A1379 YP_001217322.1 N-acetylmannosamine kinase Not tested VPI-2 Protein 6e-27 41
VPI2_0030 ACA01847.1 ROK family protein Not tested VPI-2 Protein 5e-27 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ECBD_0525 YP_003034780.1 N-acetylmannosamine kinase VFG1115 Protein 2e-27 41