Gene Information

Name : ECBD_4038 (ECBD_4038)
Accession : YP_003038207.1
Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG
Genome accession: NC_012947
Putative virulence/resistance : Unknown
Product : thiamine biosynthesis protein ThiC
Function : -
COG functional category : H : Coenzyme transport and metabolism
COG ID : COG0422
EC number : -
Position : 4238323 - 4240218 bp
Length : 1896 bp
Strand : +
Note : required for the synthesis of the hydromethylpyrimidine moiety of thiamine

DNA sequence :
ATGTCTGCAACAAAACTGACCCGCCGCGAACAGCGCGCCCAGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTT
TCCCAACTCAAAACGCATTTATATCACTGGCACACAACCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGA
CGCTAATTGGCGGCAGCAAAGAACAGCCGCAGTACGAAGAAAACGAAGCGATTCCGGCCTACGACACCTCCGGCCCGTAT
GGCGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCATGGATCGATGCGCGCGGCGATAC
CGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAGCTACGTTTTAGCG
GCTTATTAACGCCAAAACGCGCCAAAACAGGACGCCGCGTCACCCAACTGCACTACGCCCGCCAGGGCATCATCACACCG
GAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAAGTTTTACGCCACCAGCATCCGGG
AATGAGCTTTGGCGCACGTCTGCCGGAAAATATCACTGCGGAATTTGTCCGTGATGAAGTTGCTGCCGGACGTGCGATTA
TCCCGGCCAACATTAATCATCCGGAATCGGAGCCGATGATTATTGGCCGTAACTTCCTGGTAAAAGTTAACGCCAATATC
GGCAACTCAGCGGTCACCTCTTCCATCGAAGAAGAAGTGGAAAAGCTGGTATGGTCCACCCGCTGGGGAGCGGATACGGT
GATGGATCTCTCCACCGGTCGCTATATTCACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAG
TGCCGATCTACCAAGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGCGTTCCGCGACACGCTGCTG
GAACAGGCCGAGCAAGGTGTGGATTACTTCACTATCCATGCGGGCGTACTGCTGCGCTATGTGCCGATGACCGCGAAACG
CCTGACCGGTATCGTCTCTCGCGGCGGTTCGATTATGGCGAAATGGTGCCTCTCCCATCATCAGGAAAATTTCCTCTATC
AACACTTCCGCGAAATTTGTGAAATCTGTGCCGCTTATGACGTTTCGCTGTCGCTGGGCGACGGTCTGCGCCCCGGTTCT
ATTCAGGACGCCAACGATGAAGCGCAGTTTGCCGAGCTGCATACGCTGGGCGAACTGACCAAAATTGCCTGGGAATATGA
CGTGCAGGTGATGATTGAAGGCCCAGGCCACGTGCCGATGCAGATGATCCGCCGCAATATGACCGAGGAGTTAGAGCACT
GCCACGAAGCGCCGTTTTACACTCTGGGGCCGCTAACTACCGATATTGCGCCGGGCTATGACCACTTCACGTCGGGGATT
GGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAA
AGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCCGCCCACGCCGCTGACCTGGCGAAAGGGCATCCGGGCGCGC
AAATTCGCGATAACGCCATGTCGAAAGCCCGCTTCGAATTTCGCTGGGAAGACCAGTTTAATCTGGCCCTCGACCCGTTT
ACCGCCCGCGCTTATCACGATGAAACCCTGCCGCAAGAGTCAGGTAAAGTCGCCCATTTTTGCTCCATGTGTGGGCCGAA
ATTCTGCTCGATGAAAATCAGCCAGGAAGTGCGTGATTACGCCGCCACGCAAACTATTGAAATGGGAATGGCGGATATGT
CGGAGAACTTCCGTGCCAGAGGCGGAGAAATCTACCTGCGTAAGGAGGAAGCGTGA

Protein sequence :
MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRVPMREIQLSPTLIGGSKEQPQYEENEAIPAYDTSGPY
GDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEIYLRKEEA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00317 YP_008207782.1 Thiamine biosynthesis protein ThiC Not tested AbaR25 Protein 0.0 69
BJAB0868_00321 YP_008211647.1 Thiamine biosynthesis protein ThiC Not tested AbaR26 Protein 0.0 69
thiC YP_001598318.1 thiamine biosynthesis protein ThiC Not tested MDA Protein 0.0 68