Gene Information

Name : ECBD_3475 (ECBD_3475)
Accession : YP_003037659.1
Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG
Genome accession: NC_012947
Putative virulence/resistance : Unknown
Product : glutamyl-Q tRNA(Asp) synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG0008
EC number : 6.1.1.17
Position : 3620758 - 3621684 bp
Length : 927 bp
Strand : +
Note : this tRNA synthetase lacks the tRNA anticodon interaction domain; instead this enzyme modifies tRNA(Asp) with glutamate by esterifying glutamate to the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of queosine generating a modified nucleoside at the

DNA sequence :
ATGCTCCCGCCTTATTTTTTGTTCAAAGAGATGACAGACACACACTATATTGGCCGCTTCGCCCCCTCTCCTTCCGGCGA
GCTTCATTTTGGTTCTCTGATTGCTGCGCTTGGCAGCTATTTGCAGGCTCGCGCCCGGCAAGGTCGCTGGCTGGTACGTA
TAGAAGATATCGACCCGCCTCGTGAAGTTCCCGGTGCCGCAGAAACTATCCTGCGCCAGCTGGAACATTACGGTCTGCAC
TGGGACGGCGATGTTCTCTGGCAATCGCAACGTCACGACGCCTATCGTGAAGCACTCGCCTGGTTACATGAGCAAGGGCT
AAGTTATTACTGCACCTGCACGCGTGCGCGTATTCAAAGCATTGGCGGTATTTACGACGGTCATTGCCGGGTGTTGCATC
ATGGACCAGACAACGCCGCAGTGCGTATCCGCCAGCAGCATCCGGTCACGCAATTTACTGACCAGCTGCGCGGCATTATT
CACGCCGACGAAAAACTGGCACGGGAAGATTTTATTATTCATCGCCGTGATGGTTTGTTTGCCTATAACCTGGCGGTGGT
GGTGGATGATCACTTCCAGGGCGTGAGCGAAATTGTCCGCGGGGCAGATTTGATCGAACCGACAGTGCGGCAAATCTCGC
TGTACCAGCTTTTTGGCTGGAAAGTGCCAGATTACATTCATCTGCCGCTGGCGCTTAATCCACAAGGCGCTAAACTTTCC
AAGCAGAATCATGCGCCTGCGTTGCCGAAAAGCGATCCACGCCCGGTACTAATCGCGGCACTTCAATTTCTGGGTCAGCA
GGCAGAAGCACACTGGCAGGATTTCAGCGTCGAGCAAATCCTCCAGTCAGCCGTCAAAAACTGGCGGCTTACCGCCGTGC
CTGAGTCAGCAATTGTAAATTCAACATTCTCAAATGCGTCATGCTGA

Protein sequence :
MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLH
WDGDVLWQSQRHDAYREALAWLHEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHLPLALNPQGAKLS
KQNHAPALPKSDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVKNWRLTAVPESAIVNSTFSNASC

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00313 YP_008207778.1 Glutamyl- and glutaminyl-tRNA synthetase Not tested AbaR25 Protein 4e-55 44
BJAB0868_00317 YP_008211643.1 Glutamyl- and glutaminyl-tRNA synthetase Not tested AbaR26 Protein 4e-55 44