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Name : ECBD_3475 (ECBD_3475) Accession : YP_003037659.1 Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG Genome accession: NC_012947 Putative virulence/resistance : Unknown Product : glutamyl-Q tRNA(Asp) synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG0008 EC number : 6.1.1.17 Position : 3620758 - 3621684 bp Length : 927 bp Strand : + Note : this tRNA synthetase lacks the tRNA anticodon interaction domain; instead this enzyme modifies tRNA(Asp) with glutamate by esterifying glutamate to the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of queosine generating a modified nucleoside at the DNA sequence : ATGCTCCCGCCTTATTTTTTGTTCAAAGAGATGACAGACACACACTATATTGGCCGCTTCGCCCCCTCTCCTTCCGGCGA GCTTCATTTTGGTTCTCTGATTGCTGCGCTTGGCAGCTATTTGCAGGCTCGCGCCCGGCAAGGTCGCTGGCTGGTACGTA TAGAAGATATCGACCCGCCTCGTGAAGTTCCCGGTGCCGCAGAAACTATCCTGCGCCAGCTGGAACATTACGGTCTGCAC TGGGACGGCGATGTTCTCTGGCAATCGCAACGTCACGACGCCTATCGTGAAGCACTCGCCTGGTTACATGAGCAAGGGCT AAGTTATTACTGCACCTGCACGCGTGCGCGTATTCAAAGCATTGGCGGTATTTACGACGGTCATTGCCGGGTGTTGCATC ATGGACCAGACAACGCCGCAGTGCGTATCCGCCAGCAGCATCCGGTCACGCAATTTACTGACCAGCTGCGCGGCATTATT CACGCCGACGAAAAACTGGCACGGGAAGATTTTATTATTCATCGCCGTGATGGTTTGTTTGCCTATAACCTGGCGGTGGT GGTGGATGATCACTTCCAGGGCGTGAGCGAAATTGTCCGCGGGGCAGATTTGATCGAACCGACAGTGCGGCAAATCTCGC TGTACCAGCTTTTTGGCTGGAAAGTGCCAGATTACATTCATCTGCCGCTGGCGCTTAATCCACAAGGCGCTAAACTTTCC AAGCAGAATCATGCGCCTGCGTTGCCGAAAAGCGATCCACGCCCGGTACTAATCGCGGCACTTCAATTTCTGGGTCAGCA GGCAGAAGCACACTGGCAGGATTTCAGCGTCGAGCAAATCCTCCAGTCAGCCGTCAAAAACTGGCGGCTTACCGCCGTGC CTGAGTCAGCAATTGTAAATTCAACATTCTCAAATGCGTCATGCTGA Protein sequence : MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLH WDGDVLWQSQRHDAYREALAWLHEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHLPLALNPQGAKLS KQNHAPALPKSDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVKNWRLTAVPESAIVNSTFSNASC |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| BJAB07104_00313 | YP_008207778.1 | Glutamyl- and glutaminyl-tRNA synthetase | Not tested | AbaR25 | Protein | 4e-55 | 44 |
| BJAB0868_00317 | YP_008211643.1 | Glutamyl- and glutaminyl-tRNA synthetase | Not tested | AbaR26 | Protein | 4e-55 | 44 |
