Gene Information

Name : ECBD_0325 (ECBD_0325)
Accession : YP_003034583.1
Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG
Genome accession: NC_012947
Putative virulence/resistance : Resistance
Product : major facilitator superfamily MFS_1
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG2814
EC number : -
Position : 356294 - 357499 bp
Length : 1206 bp
Strand : -
Note : PFAM: major facilitator superfamily MFS_1; KEGG: seh:SeHA_C4724 tetracycline resistance protein, class B

DNA sequence : 
ATGAATAGTTCGACAAAGATCGCATTGGTAATTACGTTACTCGATGCCATGGGGATTGGCCTTATCATGCCAGTCTTGCC
AACGTTATTACGTGAATTTATTGCTTCGGAAGATATCGCTAACCACTTTGGCGTATTGCTTGCACTTTATGCGTTAATGC
AGGTTATCTTTGCTCCTTGGCTTGGAAAAATGTCTGACCGATTTGGTCGGCGCCCAGTGCTGTTGTTGTCATTAATAGGC
GCATCGCTGGATTACTTATTGCTGGCTTTTTCAAGTGCGCTTTGGATGCTGTATTTAGGCCGTTTGCTTTCAGGGATCAC
AGGAGCTACTGGGGCTGTCGCGGCATCGGTCATTGCCGATACCACCTCAGCTTCTCAACGCGTGAAGTGGTTCGGTTGGT
TAGGGGCAAGTTTTGGGCTTGGTTTAATAGCGGGGCCTATTATTGGTGGTTTTGCAGGAGAGATTTCACCGCATAGTCCC
TTTTTTATCGCTGCGTTGCTAAATATTGTCACTTTCCTTGTGGTTATGTTTTGGTTCCGTGAAACCAAAAATACACGTGA
TAATACAGATACCGAAGTAGGGGTTGAGACGCAATCGAATTCGGTATACATCACTTTATTTAAAACGATGCCCATTTTGT
TGATTATTTATTTTTCAGCGCAATTGATAGGCCAAATTCCCGCAACGGTGTGGGTGCTATTTACCGAAAATCGTTTTGGA
TGGAATAGCATGATGGTTGGCTTTTCATTAGCGGGTCTTGGTCTTTTACACTCAGTATTCCAAGCCTTTGTGGCAGGAAG
AATAGCCACTAAATGGGGCGAAAAAACGGCAGTACTGCTCGGATTTATTGCAGATAGTAGTGCATTTGCCTTTTTAGCGT
TTATATCTGAAGGTTGGTTAGTTTTCCCTGTTTTAATTTTATTGGCTGGTGGTGGGATCGCTTTACCTGCATTACAGGGA
GTGATGTCTATCCAAACAAAGAGTCATCAGCAAGGTGCTTTACAGGGATTATTGGTGAGCCTTACCAATGCAACCGGTGT
TATTGGCCCATTACTGTTTGCTGTTATTTATAATCATTCACTACCAATTTGGGATGGCTGGATTTGGATTATTGGTTTAG
CGTTTTACTGTATTATTATCCTGCTATCGATGACCTTCATGTTAACCCCTCAAGCTCAGGGGAGTAAACAGGAGACAAGT
GCTTAG

Protein sequence : 
MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGVLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIG
ASLDYLLLAFSSALWMLYLGRLLSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQLIGQIPATVWVLFTENRFG
WNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQG
VMSIQTKSHQQGALQGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQETS
A

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
tetB AEA34667.1 tetracycline resistance determinant Not tested Not named Protein 2e-163 100
tetA(B) AEZ06045.1 tetracycline efflux protein Not tested Tn6167 Protein 3e-163 100
BJAB07104_00277 YP_008207742.1 Permeases of the major facilitator superfamily Not tested AbaR25 Protein 1e-162 100
BJAB0868_00281 YP_008211607.1 Permeases of the major facilitator superfamily Not tested AbaR26 Protein 1e-162 100
tetA(B) AEQ20905.1 tetracycline resistance protein Not tested Tn6166 Protein 1e-162 100
ABZJ_00260 YP_005524216.1 Tetracycline resistance protein, class B (TETA(B) ) (Metal-tetracycline/H(+) antiporter) Not tested AbaR22 Protein 5e-163 100
tetA(B) AFH57202.1 tetracycline resistance protein Not tested AbaR4a Protein 4e-163 100
tetA(B) AAL08445.1 tetracycline resistance protein TetA(B) Not tested SRL Protein 3e-163 99
tetA YP_005797160.1 tetracycline resistance protein, class G (TETA(G)) Not tested AbaR4e Protein 2e-159 99
tet(H) YP_005176248.1 tetracycline efflux protein, class H Not tested ICEPmu1 Protein 3e-77 54
tetA CAJ77034.1 Tetracycline resistance protein Not tested AbaR1 Protein 9e-85 51
tet(G) AAK02051.1 tetracycline resistance protein Not tested SGI1 Protein 2e-81 51
tetA(G) ABZ01843.1 TetA(G) Not tested SGI2 Protein 2e-81 51
tetA(G) AGK06974.1 TetA(G) Not tested SGI1 Protein 2e-81 51
tetA(G) AGK07104.1 TetA(G) Not tested SGI1 Protein 2e-81 51
tetA YP_006098396.1 tetracycline resistance protein Not tested Tn2411 Protein 7e-70 48
tetA(A) ACK44537.1 TetA(A) Not tested SGI1 Protein 5e-70 48
tetA(A) AGK07027.1 TetA(A) Not tested SGI1 Protein 5e-70 48
tetA(A) AGK07085.1 TetA(A) Not tested SGI1 Protein 5e-70 48
tetA CAJ77066.1 Tetracycline resistance protein Not tested AbaR1 Protein 3e-70 48
tetA(A) ACN81011.1 TetA(A) Not tested AbaR5 Protein 4e-70 48

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 V00611.gene.p01 Protein 2e-162 99
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AB084246.gene.p01 Protein 1e-163 99
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 NC_010558.1.6275971. Protein 2e-163 99
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 L06798.gene.p01 Protein 2e-91 63
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AJ250203.gene.p01 Protein 3e-82 58
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 Y19116.gene.p01 Protein 1e-78 55
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 L06940.gene.p01 Protein 1e-79 55
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 Y15510.gene.p01 Protein 4e-78 54
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AF038993.gene.p01 Protein 9e-79 54
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 CP004022.1.gene2534. Protein 1e-78 54
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 NC_010410.6002612.p0 Protein 5e-85 51
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AF133140.gene.p01 Protein 5e-85 51
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AF133139.gene.p01 Protein 2e-83 51
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 NC_011586.7045189.p0 Protein 3e-66 48
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 NC_010410.6002597.p0 Protein 2e-70 48
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 CP001485.1.gene2821. Protein 2e-70 48
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AF534183.gene.p01 Protein 2e-70 48
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 Y19114.gene.p01 Protein 6e-69 47
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 X75761.gene.p01 Protein 5e-70 47
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AF121000.gene.p01 Protein 1e-62 45
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AF070999.gene.p01 Protein 1e-67 45
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AY264780.2.gene3.p01 Protein 1e-62 44
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AJ420072.gene.p01 Protein 5e-59 44
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 AY743590.gene.p01 Protein 1e-59 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ECBD_0325 YP_003034583.1 major facilitator superfamily MFS_1 VFG1036 Protein 2e-163 99