Gene Information

Name : entE (ECBD_3060)
Accession : YP_003037252.1
Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG
Genome accession: NC_012947
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 3194217 - 3195827 bp
Length : 1611 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCT
GACCGACATTCTGACTCGCCACGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTACCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGCTGTTCAGCCA
TCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTA
GCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCACAACGACAGCGGTGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCTGAGGATTTTACTGCCACGCCATCACCTGCTGATGAAGTGGCCTATTT
CCAGCTTTCCGGCGGCACCACCGGCACACCGAAACTGATCCCGCGCACTCATAACGACTACTACTACAGCGTGCGTCGTA
GCGTCGAGATTTGTCAGTTCACACAACAGACACGCTACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCG
CCGGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTTTGCTTCCCATT
GATTGAAAAACATCAGGTGAACGTCACCGCGCTGGTGCCACCGGCAGTCAGCCTGTGGTTGCAGGCGCTGACCGAAGGTG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCAGCCACCCTTGCGGCGCGTATT
CCCGCTGAGATTGGCTGCCTGTTGCAGCAGGTGTTTGGCATGGCGGAAGGGCTGGTGAACTACACCCGACTTGATGATAG
CGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGACGATGAAGTATGGGTTGCTGATGCTGAAGGAAATC
CACTGCCGCAAGGGGAAGTTGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGTTATTACAAAAGTCCGCAGCAC
AATGCCAGTGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCC
ATCCGGCGGTGATCTATGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT
GGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCG
CATCAGCCTGA

Protein sequence : 
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCLLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-88 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-88 43
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-88 43
ybtE CAA21394.1 - Virulence HPI Protein 5e-88 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 5e-88 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-87 43
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-87 43
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-88 43
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-88 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_003037252.1 enterobactin synthase subunit E VFG0932 Protein 0.0 99
entE YP_003037252.1 enterobactin synthase subunit E VFG0168 Protein 7e-116 46