Gene Information

Name : dadX (ECBD_2432)
Accession : YP_003036643.1
Strain : Escherichia coli Escherichia coli BL21-Gold(DE3)pLysS AG
Genome accession: NC_012947
Putative virulence/resistance : Unknown
Product : alanine racemase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG0787
EC number : 5.1.1.1
Position : 2554288 - 2555358 bp
Length : 1071 bp
Strand : -
Note : catabolic enzyme, converts L-alanine to D-alanine which is then oxidized to pyruvate by dadA

DNA sequence :
ATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCA
CGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGCTCGGGGCCACCG
ATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAA
GGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAA
AGCACTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCC
AGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTT
GCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCG
TTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATG
GCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATT
GGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCAT
TGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCA
TGACGGTGGGAACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTT
GAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGATGTGCGCGCT
GGCGCTACGCGTCCCGGTTGTGACGGTGTAA

Protein sequence :
MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
dadX AAC61705.1 alanine racemase Not tested PAI I CFT073 Protein 1e-164 99