Gene Information

Name : PC1_0463 (PC1_0463)
Accession : YP_003016055.1
Strain : Pectobacterium carotovorum PC1
Genome accession: NC_012917
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 528114 - 529757 bp
Length : 1644 bp
Strand : +
Note : TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase; KEGG: eca:ECA0478 enterobactin synthase subunit E

DNA sequence : 
ATGAGCATTGAATTTACGCCCTGGCCAGAGGAACTGGCGCAGCGTTACCGCGACAAGGGATACTGGGTCGGTCTGCCGTT
AACGGATGCCTGGGAACGCCATCTGACGACACAGCCGGATGCGGTTGCGGTGGTGTGCGGCGAACGTCAGTGGAGCTATC
GCGAGCTGGATCGGCAATCTTCCGCGCTGGCATCGCGCCTGACGGAAAGCGGCCTGCGCTGTGGCGACACCGCGCTGGTG
CAGTTGCCGAACGTGGCCGAGTTTTACCTGACCTTTTTTGCGTTGCTGAAAATGGGCGTCGCGCCGGTTAACGCGCTGTT
TAGCCACAACAAGCTGGAATTGCTGTCATACGCCACACAGATCGAACCGCGATTACTGATCGCCTCGGCCGAACACCCGC
TGTTTGGCGACGGCGCGTTTCTGGATCGGCTACAGACGCAGGTTCCCCGCCTGCAAACGGTGGTGATGCTTGGCGACAGC
TCGCTGGGGCACAGTCTGTCGGACTGGCTGCAACCGCGCGCTTCAACCAGCCAGTATCAGCCTTCCGCATCGGGGCAGGT
TGCCTTCTTCCAGCTTTCTGGCGGCAGTACCGGTACGCCAAAGCTGATTCCCCGTACTCATGACGATTACTACTACAGCG
TGCGCCGCAGCGTGGAAATCTGCGAGCTGACGCCGCAAACCCGCTACCTGTGTGCGTTGCCAGCACCGCATAACTTCCCG
TTAAGCTCGCCCGGTTCGCTCGGCGTGTTTTACGCCGGCGGGCGTGTCGTGCTGGCGCCCGATCCGGGGGCGATGACCTG
CTTCCCGCTGATCGAACGTCATCAGATCGATATTACCTCGTTGGTGCCGCCCGCAGCCGCGCTGTGGATTCAGGCGGCCG
AACAGTTTGGTGGGGCGCTACGTAGCCTGAAGATCTTGCAGGTGGGCGGTGCGAAGCTGAGTGAGACCGTCGCCCGTCGT
ATTCCGGCGGTGTTGGGCTGCCAGCTACAGCAGGTGCTCGGGATGGCCGAAGGGCTGGTGAACTACACCCGACTGGACGA
CAGTGATGAACATATCTTCACTACGCAGGGCTGCCCGATGAGCCCAGACGATGAAGTGAAGGTGTTGGACATCGACGGCA
ATCCGTTGCCGAGAGGCGAGGCGGGGCTGCTGGCGACGCGCGGCCCGTATACCTTCCGCGGCTATTACCGCAGCCCCGAA
CACAACGCCCGCGCCTTCGACAGCGAAGGTTTTTACCACTCGGGTGATGTGGTGCAGATGACCGAGGATGGCTATTTGTG
CGTGGTCGGGCGGGAGAAAGATCAGATTAACCGTGGTGGTGAAAAGATTGCCGCGGAAGAGATCGAAAACCTGTTGCTCA
AGCATGACGGTATTCTTCATGCCGCGCTGGTGTCGATGCCTGACCCGGTTATGGGCGAAAAGAGCTGCGCTTTTCTGGTG
GTCAGCGATCCTGCGCTGAAAGCCATTACGTTAAGAAAATATCTTCGCAATCAGGGTATTGCTGAATTCAAACTGCCGGA
TCGCTTCGAGATGATCGACACCTTGCCCGTTACGCCGGTCGGCAAGATTGATAAGAAATCACTCCGTCAGCGCATCCAGA
CCCAACTGAGTACTCAAAACCCCACTGAGCTTCAAAATAAATAA

Protein sequence : 
MSIEFTPWPEELAQRYRDKGYWVGLPLTDAWERHLTTQPDAVAVVCGERQWSYRELDRQSSALASRLTESGLRCGDTALV
QLPNVAEFYLTFFALLKMGVAPVNALFSHNKLELLSYATQIEPRLLIASAEHPLFGDGAFLDRLQTQVPRLQTVVMLGDS
SLGHSLSDWLQPRASTSQYQPSASGQVAFFQLSGGSTGTPKLIPRTHDDYYYSVRRSVEICELTPQTRYLCALPAPHNFP
LSSPGSLGVFYAGGRVVLAPDPGAMTCFPLIERHQIDITSLVPPAAALWIQAAEQFGGALRSLKILQVGGAKLSETVARR
IPAVLGCQLQQVLGMAEGLVNYTRLDDSDEHIFTTQGCPMSPDDEVKVLDIDGNPLPRGEAGLLATRGPYTFRGYYRSPE
HNARAFDSEGFYHSGDVVQMTEDGYLCVVGREKDQINRGGEKIAAEEIENLLLKHDGILHAALVSMPDPVMGEKSCAFLV
VSDPALKAITLRKYLRNQGIAEFKLPDRFEMIDTLPVTPVGKIDKKSLRQRIQTQLSTQNPTELQNK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 3e-85 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 7e-85 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-85 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-84 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-85 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-85 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 3e-85 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-85 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-85 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
PC1_0463 YP_003016055.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 4e-164 63
PC1_0463 YP_003016055.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 2e-108 45