Gene Information

Name : lysS (Dd1591_3422)
Accession : YP_003005713.1
Strain : Dickeya zeae Ech1591
Genome accession: NC_012912
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 3890805 - 3892322 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAACCACAAAACCAGGGTGCCGAGCAGGCGCTGGATCTTAACAACGAACTCAGAACCCGCCGTGAGAAGCTGGC
GGCGTTGCGTGATACCGGCATCGCTTTTCCGAACGATTTCCGCCGTGACAGCACCGCTGATCAACTACATGCCAGCTATG
ATGAAAAAGACAACGAAGAGCTGGAAGCGCTGGGCCTGGAAGTGACGGTCGCCGGCCGCATGATGACCCGCCGTATCATG
GGCAAAGCGTCGTTCGTGACTTTGCAGGACGTTGGCGGCCGCATTCAGTTGTACGTGGCGCGTGATGACCTGCCGGAAGG
CGTCTACAACGAGCAATTTAAAAAGTGGGATCTCGGCGATATCCTCGGTGCGCGCGGTAAGCTGTTTAAAACCAAGACTG
GCGAGCTGTCGATTCACTGTACCGAACTGCGTTTGCTGACCAAGGCATTGCGCCCGCTGCCGGACAAATTCCACGGCCTG
GCGGATCAGGAAACCCGCTATCGCCAGCGTTATCTGGACCTGATCGCCAACGACGAATCCCGTCACACTTTCCGCGTCCG
CTCGCAGATCCTGGCCGGTATCCGCCAGTTCATGGTCGGTCGTGATTTTATGGAAGTGGAAACGCCGATGATGCAGGTGA
TCCCCGGCGGTGCGTCTGCGCGTCCGTTCATCACCCACCACAACGCGCTGGATATCGACATGTACCTGCGTATCGCGCCG
GAATTGTATCTGAAGCGTCTGGTGGTCGGTGGTTTTGAGCGTGTATTCGAGATCAACCGTAACTTCCGTAACGAAGGTGT
GTCGCCGCGCCACAATCCTGAGTTCACCATGATGGAACTCTACATGGCGTATGCGGACTACAAAGACCTGATCGAACTGA
CCGAGTCGCTGTTCCGCACGCTGGCGCAGAATGTTCTGGGCACCACCGAGGTGCCATATGGCGACCAGGTGTTCGACTTC
GGCAAGCCGTTCGAGAAGCTGACCATGCGCGAAGCCATCAAGAAATACCGCCCGGAAACCAATCTGGCGGATCTGGACAG
CTTCGACGCGGCAAAAGCCATCGCGGAATCTATCGGCATCAAGGTAGAGAAAAGCTGGGGGCTGGGCCGTATCGTTACCG
AGATCTTCGAAGAAGTGGCGGAAGCCCATCTGATCCAGCCGACGTTCATCACCGAATACCCGGCGGAAGTCTCTCCGCTG
GCGCGTCGTAACGACCAAAACCCGGAAATCACCGATCGCTTCGAATTCTTCATCGGCGGACGTGAAATCGGTAACGGTTT
CTCCGAGTTGAACGACGCTGAAGATCAGGCGCAACGCTTCCAGGATCAAGTGGCGGCGAAAGATGCCGGCGATGACGAGG
CGATGTTCTACGACGAAGACTATGTCACCGCACTGGAGCATGGTTTGCCGCCGACCGCAGGTCTGGGCATTGGTATTGAC
CGTATGGTGATGCTGTTTACCAACAGCCACACCATTCGCGATGTGATCCTGTTCCCGGCGATGCGTCCGCAGAAATAA

Protein sequence :
MSEPQNQGAEQALDLNNELRTRREKLAALRDTGIAFPNDFRRDSTADQLHASYDEKDNEELEALGLEVTVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGDILGARGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
ADQETRYRQRYLDLIANDESRHTFRVRSQILAGIRQFMVGRDFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQNVLGTTEVPYGDQVFDF
GKPFEKLTMREAIKKYRPETNLADLDSFDAAKAIAESIGIKVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPL
ARRNDQNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFQDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-171 70
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-93 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_003005713.1 lysyl-tRNA synthetase VFG1668 Protein 6e-172 70