Gene Information

Name : entE (Dd1591_1317)
Accession : YP_003003659.1
Strain : Dickeya zeae Ech1591
Genome accession: NC_012912
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 1490449 - 1492077 bp
Length : 1629 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGATGATTGAATTCACCCGCTGGCCGCCCGAGCTGGAACAGCGCTATCGCGAACGCGGCTACTGGCTGGATGTGCCGCT
GGGCGACATTCTCACCCGGCACGCCGACAATGACGACACCGCCGTGATCGACGGCGACCGCACGTTCAGTTACCGCCAGA
TGAACCAATTGGCGGCGCAACTGGCCGGCGCGCTGCAACGCCGGGGAGTCACGCCGGGGCAAACCGCGCTGGTACAACTG
GGCAATGTTGCCGAGTTCTACCTGACTTACTTCGCGCTGCTGCGCGCCGGCATTGTGCCGGTGAACGCCCTGTTCAGCCA
TCAGCGCAGCGAGCTGTGCGCCTATGCCGACCAGATCGCCCCCACGTTGTTAATCGCCGATCGTAGCCATTCGTTGTTTA
CCGATGACCGGTTTATCGACGAGCTACGCCAACGTTGCCCGTCGTTGCGGCAAGTCATCCTGCGTGGCGACGCCGGCTCG
GCGTTATCGCTGGCGCAGCTGTTACAGGAAGACGGCGGCTCGATGGACGCTACGCCGACGCCCGCCGATCAGGTGGCGTT
TTTCCAGCTTTCCGGCGGCAGCACCGGCACCCCCAAACTGATTCCCCGTACGCACAACGATTACTACTACAGCATTCGCG
GCAGCGTAGATATTTGCCAGGTGGATGCGCACACCCGTTTCCTGTGCGCCCTGCCCGCCGCCCATAACTACCCGCTCAGT
TCGCCGGGCGCGCTAGGGGTGTTCTATGCCGGCGGTACGGTGGTGCTGGCGGGCGACCCGAGCCCAACCTGTTGCTTCCC
GTTGATTGCACGGCACCGCATCACGCTGACCGCGCTGGTGCCGCCCGCCGTCACGCTGTGGCTGGAAGCGGTGGCGTTAT
CCGGCGATCGCAGCGCGCTGGCGAGCCTGCGCGTATTGCAGGTCGGCGGCGCGCGGTTAAGCGAATCACTGGCGCGACGC
ATACCGGCGGAACTGGGCTGCCAGTTGCAACAGGTGTTCGGCATGGCGGAAGGGCTGGTGAACTATACCCGGCTGGATGA
TGACGATGTCCACCGCTTCACCACGCAGGGCCGGCCTATCAGCGACGATGACGAAGTATGGGTAGCCGATGCCGACGGCA
ATCCGTTGCCGCACGGTGAGCCGGGCCGCCTGATGACGCGCGGGCCTTATACCTTCCGCGGCTACTACCGCAGCCCGGCG
TACAACCGCCAGGTTTTTGACGCCGAGGGCTTCTACTGTTCCGGCGATCTGGTGATCCAACAGGCGGACGGCCATCTACA
GGTGGTGGGGCGCGAGAAAGATCAGATTAACCGCGGCGGGGAAAAAATAGCCGCCGAAGAGATCGAAAACCTGTTGTTGC
GCCACCCATTGGTTATCAACGCCGCGCTGGTGGCGGTGCCTGACCCGATGATGGGCGAGAAAAGCTGTGCGTTTCTGGTC
ACCCGCGAACCGCTCAAAGCGGTGGTGTTGCGTCGTCATCTGCGCGAACAGGGCATCGCCGACTACAAATTACCGGACCG
ATTTGAACAACTCCCCACCCTGCCGGAAACCGCGGTGGGTAAAGTCGATAAACAGCGGCTACGCCAGCTGGCGCAACAGC
GCGTAGCGGCGGCCACTGCAGGAGAATAA

Protein sequence :
MMIEFTRWPPELEQRYRERGYWLDVPLGDILTRHADNDDTAVIDGDRTFSYRQMNQLAAQLAGALQRRGVTPGQTALVQL
GNVAEFYLTYFALLRAGIVPVNALFSHQRSELCAYADQIAPTLLIADRSHSLFTDDRFIDELRQRCPSLRQVILRGDAGS
ALSLAQLLQEDGGSMDATPTPADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRGSVDICQVDAHTRFLCALPAAHNYPLS
SPGALGVFYAGGTVVLAGDPSPTCCFPLIARHRITLTALVPPAVTLWLEAVALSGDRSALASLRVLQVGGARLSESLARR
IPAELGCQLQQVFGMAEGLVNYTRLDDDDVHRFTTQGRPISDDDEVWVADADGNPLPHGEPGRLMTRGPYTFRGYYRSPA
YNRQVFDAEGFYCSGDLVIQQADGHLQVVGREKDQINRGGEKIAAEEIENLLLRHPLVINAALVAVPDPMMGEKSCAFLV
TREPLKAVVLRRHLREQGIADYKLPDRFEQLPTLPETAVGKVDKQRLRQLAQQRVAAATAGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-80 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-80 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-80 42
ybtE CAA21394.1 - Virulence HPI Protein 2e-80 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-80 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 8e-80 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-80 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-79 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-80 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_003003659.1 enterobactin synthase subunit E VFG0932 Protein 6e-168 68
entE YP_003003659.1 enterobactin synthase subunit E VFG0168 Protein 3e-100 45
entE YP_003003659.1 enterobactin synthase subunit E VFG1817 Protein 2e-84 43