Gene Information

Name : entE (Dd703_3074)
Accession : YP_002988667.1
Strain : Dickeya dadantii Ech703
Genome accession: NC_012880
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 3610224 - 3611834 bp
Length : 1611 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGACTATCGCGTATCACCGCTGGCCTAACGCGTTGGCAGCCCGCTATCGGGCTAAAGGTTACTGGATAGATCTGCCACT
GACAGACGTGATAACACGGCATGTGGATACCGAACGGGTCGCACTGACCGATAATGGCAAAAACTACAGTTATGCGGAAC
TCCATCGCTTGTCTAATCAGTTGGCTGCCGCACTTGCGCAACGCGGACTTCGGCGTGGTGACACGGCGCTGATCCAATTA
GGCAATATCGCCGAATTTTATATTGTCTTCTTCGCCCTGTTGAAAATCGGCGTAGCGCCAGTCAATGCGCTATTTAGCCA
CCAGCGGCACGAAATGGATGCCTACGCCCTGCAGATTCACCCCCGCGCGCTGATTGCCGATCGCCAACATAAACTATTTC
ACGATAATACCTATCTGCAGCAGTTACGCCACGCACACCCAAACCTGAACATTGTGGCCTTTCACCATCAGGATACGGGC
GATGAGTCACTGTCCATGCTGATACAACAAGCAGACACTGGCTTTGTTCCTTCGCCATCAGCGGCAGATGAGGTCGCGTT
TTTCCAGCTTTCCGGCGGCAGCACCGGGACACCCAAGCTCATTCCACGCACTCACAACGATTACTATTACAGTATTCGTG
GCAGTGTCGACATCTGTCGCTTCACCCCGCAGACACGCTACCTATGCGCACTGCCCGCAGCACATAACTATCCCCTCAGT
TCCCCAGGATCACTCGGCGTATTCTATGCCAATGGCACGGTGATACTGGCACCCGATCCGAGTGCGACCACCTGCTTCGC
ATTGATTGAACGGTATCAGGTGACAGTCGCGGCACTGGTTCCTCCCGCCGCCAGTCTATGGCTACAAGCGGTCAAGGATA
GCGGCAGTCAGGCGCTGCGTTCGCTTGAACTTCTGCAGGTGGGCGGCGCGCGGCTGAGTCCGCGTGTGGCATCAGAAATC
CCTTCCCAACTAGGCTGCCAACTACAGCAAGTGTTCGGCATGGCCGAAGGGCTGGTGAATTATACTCGTCTCGACGATCC
CCAGGATCGTATTTTCACAACTCAGGGGCGTCCCATTTCACCCGACGACGACGTGTGGGTGGCTGATGACCATGGCGCGC
CTCTGCCTGCCAACCGGATCGGGCGGTTAATGACGCGAGGTCCTTACACCATTCGCGGCTATTACAACAGCCCACAGTAC
AATGCTACCGTTTTCGATGCCGACGGCTTCTACTGTTCAGGCGATCTGGTGGCCATTGATGAGGACGGTTATATCACTGT
TCATGGGCGGGAAAAGGATCAGATCAATCGTGGTGGTGAAAAGATTGCCGCAGAAGAGATCGAGAATTTACTACTAATGC
ATCCCTCAGTGACAGAAGCGGCGCTGGTGGCGATAGAGGACGAACTGATAGGAGAAAAAAGTTACGCCTTCATCATGGCG
AATGAGCCGCTAAAAAACGTCGAAATACGCCGTTTTTTACGCAGCCATGGCGTAGCGGATTACAAATTACCCGATCACGT
TGAAATTCTATCTGCTTTGCCGCTAACACCGGTCGGCAAAATAGATAAAAAACAGTTGCGTCAATTATTGGTACTGAAAA
CCATTTTATAG

Protein sequence : 
MTIAYHRWPNALAARYRAKGYWIDLPLTDVITRHVDTERVALTDNGKNYSYAELHRLSNQLAAALAQRGLRRGDTALIQL
GNIAEFYIVFFALLKIGVAPVNALFSHQRHEMDAYALQIHPRALIADRQHKLFHDNTYLQQLRHAHPNLNIVAFHHQDTG
DESLSMLIQQADTGFVPSPSAADEVAFFQLSGGSTGTPKLIPRTHNDYYYSIRGSVDICRFTPQTRYLCALPAAHNYPLS
SPGSLGVFYANGTVILAPDPSATTCFALIERYQVTVAALVPPAASLWLQAVKDSGSQALRSLELLQVGGARLSPRVASEI
PSQLGCQLQQVFGMAEGLVNYTRLDDPQDRIFTTQGRPISPDDDVWVADDHGAPLPANRIGRLMTRGPYTIRGYYNSPQY
NATVFDADGFYCSGDLVAIDEDGYITVHGREKDQINRGGEKIAAEEIENLLLMHPSVTEAALVAIEDELIGEKSYAFIMA
NEPLKNVEIRRFLRSHGVADYKLPDHVEILSALPLTPVGKIDKKQLRQLLVLKTIL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 7e-84 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-83 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-84 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-83 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 9e-84 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-84 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 7e-84 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-84 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-84 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_002988667.1 enterobactin synthase subunit E VFG0932 Protein 7e-175 65
entE YP_002988667.1 enterobactin synthase subunit E VFG0168 Protein 2e-105 45
entE YP_002988667.1 enterobactin synthase subunit E VFG1817 Protein 1e-85 41