Gene Information

Name : entE (Dd703_1489)
Accession : YP_002987111.1
Strain : Dickeya dadantii Ech703
Genome accession: NC_012880
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 1758824 - 1760452 bp
Length : 1629 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGACGCTCGCATTTACCCGTTGGCCGGCGGCGCTCGCGCACCGTTACCGCGAGCGCGGCTACTGGGACGACCTGCCGCT
GTGCGACATCATTGCCCGTCACGCCGACAGTGAGGCGACGGCACTGATCTGCGGCGAGCGTCGGTTCAGCTATGCGCGCA
TGCAGCAGTTGAGCGCCCGTCTGGCGGCGGCGCTGCGGCGACACGGCGTACTGCCGGGGCAAACCGCGCTGGTGCAATTG
GGCAATACCGCTGAATTCTATCTGACCTTCTTCGCCCTGTTGCGGGCCGGCGTCGTGCCGGTCAACGCCTTATACAATCA
CCAACGCAGCGAACTGCGCGCTTACGCCGACCAGATAGCGCCGGCGCTGCTGATCGCCGATCGTCGCCACCCCCTGTTTG
CGGATAATGATTTTATCGACGAACTGCGCCAGCGCTGCCCGACGCTACAAACCAGTATCTTGCTCGGTCATGAAGATGAG
ACGCGGGATTTGGCCCGTCTGCTGTACGACGAAGCCCCCCTCTCATGGGAAGACACCCCCACTCCGGCGGATGAAGTGGC
GTTCTTCCAGCTATCCGGCGGCAGTACCGGGACACCCAAACTGATCCCCCGAACCCACAACGATTACTACTACAGCATCC
GTGGCAGCGTGGAGATTTGCCGTTTCGACCGGCAGACCCGCTATCTGTGCGCGCTGCCCGCCGCCCACAACTACCCGCTC
AGTTCGCCGGGCGCGCTGGGCGCGTTCTATGCGGGCGGCGCCGTCGTACTGGCGAACAACCCCAGCGCCAGCTGCTGTTT
CCCGCTGATCGCCCGCCACCGCATCAACGTCACCGCGTTGGTGCCGCCGGCCGTCACGCTATGGCTGGAGGCGGTAACAT
TGGCCGGACAACGCCACGCACTGGAGAGCCTGCAACTGCTACAAGTGGGCGGCGCGCGGCTGAGCGAATCGCTGGCGCGC
CGCATTCCGGCGGAGCTCGGATGCCAGTTACAACAGGTGTTCGGCATGGCGGAAGGGTTGGTGAATTACACCCGTCTGGA
TGATGACGAGCAGCATATCTTCACCACGCAGGGGCGCCCCATCAGCCCCGACGACGAAGTATGGGTCGCCGACGCCGACG
GCAACCCGCTGCCGCACGGCCAGGCCGGCCGTCTGATGACCCGCGGCCCTTATACCTTCCGCGGCTACTACCGTAGCCCA
GAGCACAACCGCCAGGCGTTCGACGAACACGGGTTCTACTGTTCCGGCGATCTGGTGATCCTGCGGGAAGACGGGTATCT
GCAGGTGGTCGGCCGCGAGAAGGATCAAATCAACCGCGGCGGCGAAAAGATTGCCGCCGAAGAGGTGGAAAACCTGCTGC
TGCGTCACCCGGCGGTGATCAATGCCGCGCTGGTCGCCATTCCCGATACGCTGATGGGTGAAAAAAGCTGTGCGTTCATC
GTCACCCGCGAGCCCGTTAAGCCCGTCGTGCTGCGCCGCCATCTGCGTGAGCAAGGCATCGCCGACTACAAATTGCCGGA
TCGTTTCGAAACCCTGCCGTTACTGCCGGAAACCGCCGTCGGCAAAGTGGATAAAAAGCAGTTGCGCCAGCTGGCCGAAC
AGCGTCTGGCGGCAACCGCAGGAGAGTAA

Protein sequence :
MTLAFTRWPAALAHRYRERGYWDDLPLCDIIARHADSEATALICGERRFSYARMQQLSARLAAALRRHGVLPGQTALVQL
GNTAEFYLTFFALLRAGVVPVNALYNHQRSELRAYADQIAPALLIADRRHPLFADNDFIDELRQRCPTLQTSILLGHEDE
TRDLARLLYDEAPLSWEDTPTPADEVAFFQLSGGSTGTPKLIPRTHNDYYYSIRGSVEICRFDRQTRYLCALPAAHNYPL
SSPGALGAFYAGGAVVLANNPSASCCFPLIARHRINVTALVPPAVTLWLEAVTLAGQRHALESLQLLQVGGARLSESLAR
RIPAELGCQLQQVFGMAEGLVNYTRLDDDEQHIFTTQGRPISPDDEVWVADADGNPLPHGQAGRLMTRGPYTFRGYYRSP
EHNRQAFDEHGFYCSGDLVILREDGYLQVVGREKDQINRGGEKIAAEEVENLLLRHPAVINAALVAIPDTLMGEKSCAFI
VTREPVKPVVLRRHLREQGIADYKLPDRFETLPLLPETAVGKVDKKQLRQLAEQRLAATAGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 5e-85 45
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 4e-85 45
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 4e-84 45
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-85 45
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-84 45
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-85 45
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-85 45
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-85 45
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-85 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_002987111.1 enterobactin synthase subunit E VFG0932 Protein 2e-172 67
entE YP_002987111.1 enterobactin synthase subunit E VFG0168 Protein 2e-106 48
entE YP_002987111.1 enterobactin synthase subunit E VFG1817 Protein 3e-82 42