Gene Information

Name : Rleg_0728 (Rleg_0728)
Accession : YP_002974570.1
Strain : Rhizobium leguminosarum WSM1325
Genome accession: NC_012850
Putative virulence/resistance : Unknown
Product : transposase IS66
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG3436
EC number : -
Position : 758698 - 760320 bp
Length : 1623 bp
Strand : +
Note : PFAM: transposase IS66; KEGG: sme:SMa2171 transposase

DNA sequence :
ATGTCTAGCGCGACGACAAATCTTCCGGACGATCCAGCCTTTCTCAAGGCAATGATTGCCGCTTTGCAGGCGGAAAACGC
GAAGATGTCGGCCACATTGCAGGCGCATGATCAGTTAATCCAAACGCTGCGGCTGCGCATTGCCAAGCTGAAGAAACAGG
TCTTCGGCAAGTCGTCTGAAAAGATTGAGCGTGAAATCGAGCAGTTGGAACTGGCGCTTGAGGATCTGTTGATCGCCGCC
GCTGAAGGCAGTACGGCGCCAATCGATGAACCTGATGAGGCGGCGTCTGTTGTTCCCTTGGCGGATACGTCTGAAAAGAT
CATGCGCCGGCGCCCACGCGTCTCGGACAAGGCGGTTCGCGAGCGCCGAGAGCTCGATCCTGGCTCCTGCTGCCCGGAAT
GCGGAGGTGAATTGCGTCTTGTCGGTGAGGACGTCAGCGAAATCCTCGACATGATCGCCGCGCAGATGAAAGTCATCGAA
GTTGCTCGACTGAAGAAGTCCTGCCGCTGCTGCGAGAAAATGGTGCAGGTGGCAGCACCCAGCCGCCCGATACCGGGCAG
CATGGCCGGCGCTGGCCTCTTGGCGTACATTCTGGTCTCGAAGTTCGACGACCATTTGCCACTGTACCGTCTGAACGAGA
TCTTCGCCCGCATGGGCGCCGACATTCCCGACAGCACGATGGTTGATTGGTGTGGTCGCGCCATGCAGGTTCTGCAGCCT
CTCATCGAGCGGATCGAAACGGCGGTCATGGCAAGTGACCTGCTTCATGCGGACGACACCCCGATCAGGGTGCTGGATCG
CTCGCTGCGAGACAAGGGGTTGGGCAAGGGTGTGAAGAAGGGCAGGATCTGGACGTATGTCCGCGATCAGCGTCCATGGG
CAGGCAGTTCCCCGCCCGGTGCAGTCTACTATTTTGCTCCTGACTGGAAAGAAGAGCACGTCCACCGCCACCTCAAGCAA
TCAAGCGGCATCCTTCAGGCTGACGGCTACAAAGGATATGGCAAGCTATACTCGCCTGGAGAAAAGGGAGAATCTCGCTT
CAAGGAAGCCGCCTGCTGGGCTCATTGGCGACGAGACTTCCACGATATCTGGACATCAAACAAGTCCGAGATCGCGCGAG
AGGCTCTTCATCGCATCGGAGCGCTTTACGACATCGAGCGTGGCATCAATGGGCAGCCTCCTGAGATCCGTCTTGCTGCG
CGTCAAAAGCAGAGCAAGCCTAAGGCCGATGCATTCCGCCACTGGGCTGAAGCTCAACTCACGCGCATTCCGGGCAAAAG
CGATCTGGCGACAGCTTTCCGCTACGGATTGAGCCGATGGTCTTCGCTCTGTCTGTTCCTCGAAGACGGTCGCGTTGCGA
TCGACAACAATGCCGCCGAGCGGGCACTGCGTCCGATAGGCGTGGGAAGACGAAACTGGCTCTTCGCGGGAGCCGATACG
GGAGCGGAGACCTTGGCACGCGCCATGACGATCATCGAGACGGCAAAGATGAATGGTCTTGATCCGCAGGCCTATCTGGC
AGACGTGCTCGATCGCATCCACGATCACAAGATCAATCGGTTAGACGAATTGCTTCCGTGGAACTGGTCGGCGATCACCA
CAATCGACGCGAAGGCAGCCTGA

Protein sequence :
MSSATTNLPDDPAFLKAMIAALQAENAKMSATLQAHDQLIQTLRLRIAKLKKQVFGKSSEKIEREIEQLELALEDLLIAA
AEGSTAPIDEPDEAASVVPLADTSEKIMRRRPRVSDKAVRERRELDPGSCCPECGGELRLVGEDVSEILDMIAAQMKVIE
VARLKKSCRCCEKMVQVAAPSRPIPGSMAGAGLLAYILVSKFDDHLPLYRLNEIFARMGADIPDSTMVDWCGRAMQVLQP
LIERIETAVMASDLLHADDTPIRVLDRSLRDKGLGKGVKKGRIWTYVRDQRPWAGSSPPGAVYYFAPDWKEEHVHRHLKQ
SSGILQADGYKGYGKLYSPGEKGESRFKEAACWAHWRRDFHDIWTSNKSEIAREALHRIGALYDIERGINGQPPEIRLAA
RQKQSKPKADAFRHWAEAQLTRIPGKSDLATAFRYGLSRWSSLCLFLEDGRVAIDNNAAERALRPIGVGRRNWLFAGADT
GAETLARAMTIIETAKMNGLDPQAYLADVLDRIHDHKINRLDELLPWNWSAITTIDAKAA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BCAM0248 YP_002232880.1 putative transposase Not tested BcenGI11 Protein 2e-89 44
Z4340 NP_289564.1 hypothetical protein Not tested OI-122 Protein 2e-84 43
st57 CAC81895.1 ST57 protein Not tested LEE II Protein 6e-80 43
s0025 CAD33772.1 IS66-like transposase Not tested PAI I 536 Protein 7e-65 41
Z1161 NP_286696.1 hypothetical protein Not tested TAI Protein 2e-83 41
Z1600 NP_287104.1 hypothetical protein Not tested TAI Protein 2e-83 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Rleg_0728 YP_002974570.1 transposase IS66 VFG1513 Protein 4e-65 41
Rleg_0728 YP_002974570.1 transposase IS66 VFG1736 Protein 2e-70 41