Gene Information

Name : Rleg_1320 (Rleg_1320)
Accession : YP_002975151.1
Strain : Rhizobium leguminosarum WSM1325
Genome accession: NC_012850
Putative virulence/resistance : Resistance
Product : Bcr/CflA subfamily drug resistance transporter
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG2814
EC number : -
Position : 1301868 - 1303055 bp
Length : 1188 bp
Strand : -
Note : TIGRFAM: drug resistance transporter, Bcr/CflA subfamily; PFAM: major facilitator superfamily MFS_1; KEGG: ret:RHE_CH01569 florfenicol/chloramphenicol resistance transporter protein

DNA sequence :
ATGTCTTATCCCAAAATCCAGCAATGGACCTATTCCCTGCCGGCAGCCTTGCTGCTTATGGCACCTTTCGACATCCTGGC
CTCGCTCGCCATGGATATCTATCTGCCGATTGTGCCGGTCATGCCGGAGGCGCTCGGCACTTCGCCGGCGGTCATCCAAC
TGACCCTCAGCCTCTACATGCTCGTGCTCGGTGTCGGCCAGATCGTCTTCGGCCCAATTTCCGATATTATCGGACGACGG
CCTGTCCTGCTTGGCGGTGCTGCACTCTTTGCCGCGTCATCCTTCCTGCTTGCCGGCTCCTCGTCGGCGCCGGTCTTCGT
GGTCTTGAGATTGCTGCAAGCAGTCGGCGCGTCGGCGACCCTCGTCGCAACTTTCGCAACCGTGCGTGACGTCTACGCCG
GGCGTCCCGAAAGCAGCACGATCTATAGCCTTCTCGGCTCCATCCTCTCTTTCGTGCCCGCACTCGGCCCGATCATCGGA
GCCGCGATCGCGGATCGCTTCGGCTGGCGCGCCATCTTCCTCGTCATCGGCCTCCTTTCGGTTGCCGCGCTTTTCAACGC
CGGTATGCGATGGCACGAAACCCGCCCTGCCGTCACCGCCAAGATCGCTCTCCGCGCGATCCTTAGAAGTCTTCCCTTCT
GGGTTTATGCGGGCGGCTTCAGCACGGCGATGGGCGCCTTCTTCGTTTTCTTCTCGACGGCACCCCGTATCCTGATCGAC
AAAGCCGGTTTTTCCGGCATCGCCTTCAGCTTCATCTTTGCCACGGTGGCGCTGGTGATGATCGTGACGGCGCGTTTCGC
CAAAGGCTTCGTCATGCGCTGGGGCATTGCAGGCAGCCTTGCCAGAGGCATGGCAATACTGCTTCTCGGCGCCGTGCTGC
TTGCCTTTGGAGAGTTGTTTCTGCTGCCTTCCCTCGTCAGCTTCGTCGTGCCGATGTGGATCATCGCGATCGGCATCGTC
GTTGCGACGGCGGTCACCGCCAACGGCGCGCTCGACGCCTTTGGCGACACAGCGGGAACGGCGGTCGCGCTCTACTTCTG
CGTCCAAAGCATCATCGTCGGCTTTGCCGGAACCTTCTTCGTGATTCTGCTCGGCGGCGACAGCGCCTGGCCACTCGTGG
GCTATGTCTCAGCGACCGCGCTGACGACGCTGGCTGGATTGTGGTGCCTGCGGGAGCCGGTCCGATAG

Protein sequence :
MSYPKIQQWTYSLPAALLLMAPFDILASLAMDIYLPIVPVMPEALGTSPAVIQLTLSLYMLVLGVGQIVFGPISDIIGRR
PVLLGGAALFAASSFLLAGSSSAPVFVVLRLLQAVGASATLVATFATVRDVYAGRPESSTIYSLLGSILSFVPALGPIIG
AAIADRFGWRAIFLVIGLLSVAALFNAGMRWHETRPAVTAKIALRAILRSLPFWVYAGGFSTAMGAFFVFFSTAPRILID
KAGFSGIAFSFIFATVALVMIVTARFAKGFVMRWGIAGSLARGMAILLLGAVLLAFGELFLLPSLVSFVVPMWIIAIGIV
VATAVTANGALDAFGDTAGTAVALYFCVQSIIVGFAGTFFVILLGGDSAWPLVGYVSATALTTLAGLWCLREPVR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
floR YP_005176183.1 florfenicol / chloramphenicol efflux protein Not tested ICEPmu1 Protein 3e-87 65
floRc AGK07102.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 9e-88 64
flo AAK02049.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 9e-88 64
floRc AGK06972.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 9e-88 64
cmlA9 ABZ01840.1 CmlA9 chloramphenicol efflux protein Not tested SGI2 Protein 1e-84 63
cmlA CAJ77032.1 chloramphenicol and florfenicol resistance protein Not tested AbaR1 Protein 1e-84 63
cml(A)5 ACY75522.1 CmLA(5) Not tested Tn6060 Protein 8e-64 49
cmlA5 CAJ77046.1 Chloramphenicol resistance protein Not tested AbaR1 Protein 1e-63 49

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Rleg_1320 YP_002975151.1 Bcr/CflA subfamily drug resistance transporter AF294653.1.gene4.p01 Protein 6e-64 50
Rleg_1320 YP_002975151.1 Bcr/CflA subfamily drug resistance transporter NC_010410.6003586.p0 Protein 5e-64 49
Rleg_1320 YP_002975151.1 Bcr/CflA subfamily drug resistance transporter DQ984668.gene.p01 Protein 5e-64 49
Rleg_1320 YP_002975151.1 Bcr/CflA subfamily drug resistance transporter DQ522237.1.gene4.p01 Protein 5e-64 49
Rleg_1320 YP_002975151.1 Bcr/CflA subfamily drug resistance transporter AF156486.gene.p01 Protein 5e-63 48