Gene Information

Name : fliI (MexAM1_META1p0507)
Accession : YP_002961725.1
Strain : Methylobacterium extorquens AM1
Genome accession: NC_012808
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 530845 - 532173 bp
Length : 1329 bp
Strand : +
Note : Evidence 2b : Function of strongly homologous gene; Product type e : enzyme

DNA sequence :
ATGACCCTCAACGCGATCGAGCGCCTGGAACGGGCGATGGAGGCCGGGCGCCGCGACCTGCCGCTGGTGCGGGTCGCCGG
CCCCGTGCGCGAGGTGACCGCCAGCGCGGCACGGGTTGCGGGCGTGTCCGCCTTCGTGCGGCTCGGCGACCGCATGGGTT
TTTCCGCCGACGGCCGCACGCAGGTCGGCGAGGTCGTCCGCATCGATGCGGCCGGCGCAACGGTGAAGCCGTTCGAGAGC
CGGATCGAGGCCGGCATCGGCAGCCTCGCCCACCGCATCGGCCCGGCGCAGCTGCGCCCGGACCCGCGCTGGAAGGGCCG
GGTGATCGACGCGCTCGGCCGCCCCATCGATGGGCTCGGCCCCCTGGCCGAGGGGCCCGCCAGCGTTCCGCTGGATGCCG
ACCCGCCCGCCGCGATGACCCGCGCCCGGGTGAAGACGCCGCTGCCCACGGGGGTGCGGGCGATCGACCTGTTCACGCCG
CTCTGCGCCGGACAGCGCATCGGCATCTTCGCCGGCTCCGGCGTCGGCAAATCGACCCTGCTGGCGATGCTGGCCGGTGC
CCAGGGCTTCGACAGCGTCGTGGTCGCGCTGGTGGGCGAGCGCGGACGCGAGGTGCGCGAGTTCCTCGAAGGCCCCATCG
CCGCCGCCCGCGACCGCGCCGTGATCGTGGTCTCCACCGGCGACGAAAGCCCGATGATGCGCCGCCAAGCGCCGAAGGTG
GCGCTGGCCGTGGCCGAGTCCTTCCGCGACCGCGGCGAGTCGGTGCTGCTCATCGTCGATTCCGTGACCCGCTACGCCCA
CGCCGCCCGCGACGTGGCGCTCGCCGCAGGCGAGCCGGCGGTGGCGCGCGGCTATCCGCCGAGCGTCTTTTCGGACTTGC
CGCGACTCCTGGAGCGCGCCGGCCCCGGCGCGGAGGGCACCGGCACGATCACCGGCATCTTCTCGGTGCTGGTCGATGGC
GACGACCATAACGACCCGGTCGCCGATTCGATCCGCGGCACCCTGGACGGCCACGTGGTGCTCGACCGCGCCATCGCCGA
GCAGGGCCGCTACCCGGCGATCGAGCTGCTCGGCTCGATCTCGCGCCTGGCGGGCGAGGTCTGGACCGGCGACCAGCGCG
AGTTGGTGCGCAAGCTCAAGAGCATGATGGCCCGCTTCGAGGAGACACGGGATCTGCGGCTGATGGGCGGCTACCGCGCC
GGCAGCGATCCCGACCTCGACCAGGCCGTCAGCCTCGTGCCGCGGATCTACGAGGCCCTGCGCCAGGAGCCGGGCCAGCC
GCCGAGCCGCGACGCCTTCCTCGAACTCGCCCAATCCCTGCGGGGATGA

Protein sequence :
MTLNAIERLERAMEAGRRDLPLVRVAGPVREVTASAARVAGVSAFVRLGDRMGFSADGRTQVGEVVRIDAAGATVKPFES
RIEAGIGSLAHRIGPAQLRPDPRWKGRVIDALGRPIDGLGPLAEGPASVPLDADPPAAMTRARVKTPLPTGVRAIDLFTP
LCAGQRIGIFAGSGVGKSTLLAMLAGAQGFDSVVVALVGERGREVREFLEGPIAAARDRAVIVVSTGDESPMMRRQAPKV
ALAVAESFRDRGESVLLIVDSVTRYAHAARDVALAAGEPAVARGYPPSVFSDLPRLLERAGPGAEGTGTITGIFSVLVDG
DDHNDPVADSIRGTLDGHVVLDRAIAEQGRYPAIELLGSISRLAGEVWTGDQRELVRKLKSMMARFEETRDLRLMGGYRA
GSDPDLDQAVSLVPRIYEALRQEPGQPPSRDAFLELAQSLRG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-58 46
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-60 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-61 45
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-61 45
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-60 45
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-60 45
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-53 45
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 4e-53 45
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-53 45
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-53 45
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 5e-63 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 6e-53 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 5e-53 44
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-63 43
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-64 43
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-64 43
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-62 43
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-62 43
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-62 43
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-52 43
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-57 43
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-57 43
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-56 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 8e-52 43
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-62 42
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-62 42
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-62 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-46 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-46 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 6e-52 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 4e-48 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-50 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_002961725.1 flagellum-specific ATP synthase VFG0192 Protein 3e-59 48
fliI YP_002961725.1 flagellum-specific ATP synthase VFG2331 Protein 9e-66 48
fliI YP_002961725.1 flagellum-specific ATP synthase VFG2144 Protein 6e-64 47
fliI YP_002961725.1 flagellum-specific ATP synthase VFG0515 Protein 8e-61 45
fliI YP_002961725.1 flagellum-specific ATP synthase VFG0555 Protein 2e-53 45
fliI YP_002961725.1 flagellum-specific ATP synthase VFG1254 Protein 3e-62 44
fliI YP_002961725.1 flagellum-specific ATP synthase VFG0048 Protein 2e-64 41
fliI YP_002961725.1 flagellum-specific ATP synthase VFG1008 Protein 8e-49 41