Gene Information

Name : fliI (GWCH70_1114)
Accession : YP_002949240.1
Strain : Geobacillus sp. WCH70
Genome accession: NC_012793
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.15
Position : 1165267 - 1166547 bp
Length : 1281 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGGACACTTATAAACGGTTTGGCAAAGTTTCACGGGTCATTGGCTTAATGATTGAAGCGAAAGGACCGGAAAGCTCAGT
CGGCGACATTTGCTATATTCACACTAGAAGCGGCAGGCAAAAACAATTGATTGCGGCAGAAGTAGTTGGATTTAAAGAGC
AACATGTATTGTTAATGCCGTTTTCGACTGTTCAGCATATTTCACCAGGATGTATTGTTGAAGCAACTGGAAAACCGTTG
CAAATTCACGTTGGCACGCCATTAATCGGGCAAGTGCTTGACCCTCTCGGCTATCCACTAGATGGCAGCCCATTGCCGAA
GGGATTGCATCCTATTTCGATTGAGCGCAATCCGCCCAATCCGCTTGCAAGGCCGCCGATCAACGAACCGATCGAAGTTG
GGGTTCGGATCATTGACAGCTTGTTAACGGTTGGCAAAGGGCAGCGCGTCGGAATTTTTGCCGGTTCGGGAGTTGGAAAA
AGCACGTTAATGGGGATGATTGCCCGTCACACGAATGCGGATATAAATGTTATCGCATTAATTGGTGAGCGCGGACGTGA
AGTCCGTGAATTTATTGAACGGGACCTCGGTCCGGAAGGTTTGGCCCGTTCTATCGTTGTCGTTGCCACTTCTGATCAGC
CTGCGCTCATGCGTGTAAAAGGAGCATATACCGCCACGGCGATTGCGGAATATTTCCGCGACCAAGGGATGGATGTGATG
TTTATGATGGACTCTGTCACACGCGTTGCCATGGCGCAGCGAGAAATTGGACTAGCCATCGGCGAGCCGCCGACAACAAA
AGGGTATACTCCGTCCGTGTTTGCTATCTTACCGAAATTATTAGAGCGTACAGGGACGAGCGCTTACGGCACTATTACCG
CATTTTATACCGTACTTGTTGACGGAGATGACATGAACGAGCCGATTGCTGATACGGTACGCGGCATTTTGGACGGACAT
TTCATCCTTGAACGAAGCTTAGCCAATAGGGGACAGTATCCAGCCATTAACGTATTGAAAAGCGTTAGCCGCGTAATGAA
TCATATTATTACACCAGAACATCGGCAAGCGGCAGATAAACTGCGCCAGCTTCTATCCACTTATATTAATTCGGAAGACT
TAATCAATATAGGGGCGTACAAACGAGGATCGTCAAACGAAATTGATGAAGCGATTCGTTATTATCCAAAAATCATTTCC
TTTTGCAAACAAGATATTCATGAAAAAATAACAAAAGACGAAAGCATTGAAATGCTAATTGACCTAATCCATGCAGGGTG
A

Protein sequence :
MDTYKRFGKVSRVIGLMIEAKGPESSVGDICYIHTRSGRQKQLIAAEVVGFKEQHVLLMPFSTVQHISPGCIVEATGKPL
QIHVGTPLIGQVLDPLGYPLDGSPLPKGLHPISIERNPPNPLARPPINEPIEVGVRIIDSLLTVGKGQRVGIFAGSGVGK
STLMGMIARHTNADINVIALIGERGREVREFIERDLGPEGLARSIVVVATSDQPALMRVKGAYTATAIAEYFRDQGMDVM
FMMDSVTRVAMAQREIGLAIGEPPTTKGYTPSVFAILPKLLERTGTSAYGTITAFYTVLVDGDDMNEPIADTVRGILDGH
FILERSLANRGQYPAINVLKSVSRVMNHIITPEHRQAADKLRQLLSTYINSEDLINIGAYKRGSSNEIDEAIRYYPKIIS
FCKQDIHEKITKDESIEMLIDLIHAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-101 55
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-101 53
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-101 53
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-102 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-101 52
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-102 52
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-102 52
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-101 52
lscN AAO18044.1 LscN Virulence TTSS locus Protein 3e-99 52
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 8e-102 51
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 3e-90 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-87 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-87 48
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-87 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 9e-88 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-88 48
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-82 48
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-82 48
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-81 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 4e-82 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-82 46
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-82 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-82 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-82 46
escN AAC31513.1 L0034 Virulence LEE Protein 3e-82 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 3e-82 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-81 46
escN AAC38383.1 EscN Virulence LEE Protein 3e-82 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 4e-82 46
escN AAK26715.1 EscN Virulence LEE Protein 2e-81 45
escN AAL57542.1 EscN Virulence LEE Protein 2e-81 45
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 5e-74 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-76 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-76 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-71 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 1e-71 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-71 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-71 44
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-73 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 6e-75 43
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-73 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-73 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-77 42
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-77 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-77 42
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-76 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-70 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 5e-72 41
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-83 41
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-68 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_002949240.1 flagellum-specific ATP synthase VFG2144 Protein 1e-103 54
fliI YP_002949240.1 flagellum-specific ATP synthase VFG0192 Protein 4e-99 52
fliI YP_002949240.1 flagellum-specific ATP synthase VFG0390 Protein 3e-101 51
fliI YP_002949240.1 flagellum-specific ATP synthase VFG2499 Protein 4e-91 51
fliI YP_002949240.1 flagellum-specific ATP synthase VFG0048 Protein 3e-96 50
fliI YP_002949240.1 flagellum-specific ATP synthase VFG1254 Protein 6e-97 50
fliI YP_002949240.1 flagellum-specific ATP synthase VFG0515 Protein 1e-87 48
fliI YP_002949240.1 flagellum-specific ATP synthase VFG2331 Protein 1e-91 48
fliI YP_002949240.1 flagellum-specific ATP synthase VFG0730 Protein 2e-82 46
fliI YP_002949240.1 flagellum-specific ATP synthase VFG0812 Protein 2e-82 46
fliI YP_002949240.1 flagellum-specific ATP synthase VFG0555 Protein 6e-72 44
fliI YP_002949240.1 flagellum-specific ATP synthase VFG1778 Protein 1e-72 43
fliI YP_002949240.1 flagellum-specific ATP synthase VFG1008 Protein 1e-71 41
fliI YP_002949240.1 flagellum-specific ATP synthase VFG2459 Protein 9e-74 41