Gene Information

Name : lysS (HDEF_0009)
Accession : YP_002922936.1
Strain : Candidatus Hamiltonella defensa T5A
Genome accession: NC_012751
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 6813 - 8327 bp
Length : 1515 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAAGAAAAAATATCTATTACAGAGCGAAAAGAACTTAATCATGAGTTACAAACTCGTCGAGAAAAATTGGCGTT
ATTACGAAAAAAAAGCTTTGCCTTCCCAAATGACTTTCGTCAGAAACATTTTTCTGGTGATTTGCATACTGAATATGATC
ATAAAGAAAACGAAGAACTTATCGCTTTAAATAAGGAAGTGATTCTTGCTGGGCGTATGATGACAAGACGTATCATGGGT
AAAGCCTCTTTTTTTACTCTTCAAGATACAAAAGGCCGAATTCAACTTTACGTTTCTCGCGATGATTTACCAGAAGGCAT
TTATAACGAAGATTTCAAAAAATGGGATTTAGGCGATATTTTAGGGGTGCGAGGTCGATTGTTTAAAACAAAAACAGGAG
AGCTTTCTGTTCATTGCACCGAATTGCGTTTACTCACGAAAGCATTGCGCCCACTGCCAGATAAATTTCACGGGCTTCTA
GATCAAGAGGCACGATACCGTCAACGTTACTTAGATTTGATCGTCAACGAAAAATCTCGAAAAAACTTTATCTTACGTTC
AAAAACAATTTCAATGCTACGCCAGTTTATGATGGGCAAAGGGTTTATGGAAGTGGAAACTCCTATGATGCAATCTATCC
CAGGAGGAGCTGCAGCTCGCCCATTTTTAACGCACCATAATGCGCTGAATATCGATATGTACTTGAGAATAGCGCCTGAA
TTGTATTTAAAACGTTTGGTGGTCGGGGGATTTGATCGCATATTTGAAATCAATCGTAATTTTCGTAATGAAGGTATTTC
ATTACGCCACAATCCTGAATTTACCATGATGGAGCTTTACATGGCGTATGCGGATCACCAGGATTTGATTGAGTTAACAG
AGGAACTTTTTCGTGAGTTAACCCTAAACGTATTAGGAACGCATGTAGTAAAATATGGCGAACATATTTTTGATTTTAGT
CAACCTTTTCAAAAATGGACAATGAAAGAAGCCATTCAAAAATATCGACCTGAAATTAATATTAACGATTTAGAAGATAA
AAATAAGGCGATACTGATTGCAGAATCTCTAGATGTTCGTGTAGAAAAATTTTGGGAATTAGGGCGAATTCAATGTGAAA
TTTTTGAAGAAACGGCAGAAAAACATCTTATTCAACCTACTTTTATTACTGAATATCCAGCAGAAGTTTCACCATTAGCG
CGTCGAAATGATCATAATTCTTTTATTGCGGATCGATTTGAATTTTTTATTGCAGGTAAAGAAATTGGCAATGGTTTTTC
TGAATTGAATGATGCTGAAGATCAAGCTCAACGTTTTACCGAACAATTAAACTCAAAAGAAAAAGGCGATCATGAAGCGA
TGTCTTATGATCAAGATTATATTATTGCACTAGAACACGGATTACCTCCGACCGCAGGTTTAGGAATAGGTATTGATCGT
ATGATTATGCTATTGACTAATAGTATAAATATCCGTGATGTTATTCTATTTCCAACAATGCGCCCATTAAAATAA

Protein sequence :
MSEEKISITERKELNHELQTRREKLALLRKKSFAFPNDFRQKHFSGDLHTEYDHKENEELIALNKEVILAGRMMTRRIMG
KASFFTLQDTKGRIQLYVSRDDLPEGIYNEDFKKWDLGDILGVRGRLFKTKTGELSVHCTELRLLTKALRPLPDKFHGLL
DQEARYRQRYLDLIVNEKSRKNFILRSKTISMLRQFMMGKGFMEVETPMMQSIPGGAAARPFLTHHNALNIDMYLRIAPE
LYLKRLVVGGFDRIFEINRNFRNEGISLRHNPEFTMMELYMAYADHQDLIELTEELFRELTLNVLGTHVVKYGEHIFDFS
QPFQKWTMKEAIQKYRPEININDLEDKNKAILIAESLDVRVEKFWELGRIQCEIFEETAEKHLIQPTFITEYPAEVSPLA
RRNDHNSFIADRFEFFIAGKEIGNGFSELNDAEDQAQRFTEQLNSKEKGDHEAMSYDQDYIIALEHGLPPTAGLGIGIDR
MIMLLTNSINIRDVILFPTMRPLK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-153 64

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002922936.1 lysyl-tRNA synthetase VFG1668 Protein 1e-153 64