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Name : lysS (GBP346_A2770) Accession : YP_002897461.1 Strain : Burkholderia pseudomallei MSHR346 Genome accession: NC_012695 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 2698533 - 2700059 bp Length : 1527 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGACCGAACCGACCCAACCGCAGGCCGCTGTCGCAGCGGACGAAAACCAGATCGTCGCGGAGCGCCGCGACAAGCTGCG CGCGCTGCGCGACCAAGGCATCGCCTATCCGAACGACTTCCAGCCGACGCACCACGCGGCCGGTCTGCAGACCGAATACG CGGACGCCGACAAGGAAGCGCTCGACGCGAAGGCGCTCGACGTCGCGATCGCCGGCCGGATGATGCTCAAGCGCGTGATG GGCAAGGCGAGCTTCGCGACGGTGCAGGACGGTTCGGGTCAAATCCAGTTCTTCGTGACGCCCGCCGATGTCGGCGCCGA AACGTACGACGCGTTCAAGAAGTGGGACCTGGGCGACATCGTCGCCGCGCGCGGCGTGCTGTTTCGCACGAACAAGGGCG AGCTGTCGGTGAAGTGCACGGAGCTGCGGCTGCTCGCGAAGGCGCTGCGTCCGCTGCCCGACAAGTTCCACGGCCTCGCC GACCAGGAAACGCGCTATCGCCAGCGCTACGTCGATCTGATCGTCACGCCCGAGACGCGCGCGACGTTCCGCGCGCGCAC GAAGGCGATCGCGTCGATCCGCAAGTTCATGAGCGACGCCGATTTCATGGAAGTCGAGACGCCGATGCTGCACCCGATCC CGGGCGGCGCGGCCGCGAAGCCGTTCGTCACGCATCACAATGCGCTCGACATGCAAATGTTCCTGCGCATCGCGCCCGAG CTTTACCTGAAGCGCCTGATCGTCGGCGGCTTCGAGCGCGTGTTCGAGATCAACCGCAACTTCCGGAACGAAGGCGTGTC GCCGCGCCACAATCCGGAATTCACGATGATGGAGTTCTACGCCGCGTACACCGATTACCGCTGGCTGATGGACTTCACCG AGCGGCTGATCCGCCAGGCGGCCGTCGACGCGCTCGGCACCGCGACGATCCGGTACCAGGGCCGCGAGCTCGATCTCGCC AAGCCGTTCCATCGCCTGACGATCACGCAGGCGATCCAGAAATACGCACCGAACTACACGGACGGTCAGTTGTCGGACGA CGCGTTCCTGCGCGGCGAACTCAAGCGCCTGGGCGTCGACGTCACGCAGCCGGCGTTCCTGAACGCCGGCATCGGCGCGC TGCAGCTCGCGCTTTTCGAGGAAACCGCCGAAGCGCAGCTGTGGGAACCGACCTTCATCATCGACTACCCGATCGAGGTG TCGCCGCTCGCGCGCGAATCGGACACGGTGGCGGGCATCACCGAGCGCTTCGAGCTGTTCGTCACCGGCCGTGAAATCGC GAACGGCTTCTCGGAACTGAACGACCCCGAAGACCAGGCCGCGCGCTTCAGGAAGCAGGTCGAGCAGAAGGACGCGGGCG ACGAAGAGGCGATGTTCTTCGACGCCGACTACATCCGCGCGCTCGAATACGGAATGCCGCCGACAGGCGGCTGCGGGATC GGCATCGACCGGCTCGTGATGCTGCTGACCGACAGCCCGACGATTCGCGACGTGCTGCTGTTCCCGCACCTGCGCCGCGA AGACTGA Protein sequence : MTEPTQPQAAVAADENQIVAERRDKLRALRDQGIAYPNDFQPTHHAAGLQTEYADADKEALDAKALDVAIAGRMMLKRVM GKASFATVQDGSGQIQFFVTPADVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVKCTELRLLAKALRPLPDKFHGLA DQETRYRQRYVDLIVTPETRATFRARTKAIASIRKFMSDADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTERLIRQAAVDALGTATIRYQGRELDLA KPFHRLTITQAIQKYAPNYTDGQLSDDAFLRGELKRLGVDVTQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPIEV SPLARESDTVAGITERFELFVTGREIANGFSELNDPEDQAARFRKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI GIDRLVMLLTDSPTIRDVLLFPHLRRED |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-126 | 55 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_002897461.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 7e-127 | 55 |
