Gene Information

Name : Tola_2273 (Tola_2273)
Accession : YP_002893457.1
Strain : Tolumonas auensis DSM 9187
Genome accession: NC_012691
Putative virulence/resistance : Virulence
Product : N-6 DNA methylase
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0286
EC number : -
Position : 2494504 - 2496897 bp
Length : 2394 bp
Strand : -
Note : PFAM: N-6 DNA methylase; KEGG: shw:Sputw3181_1087 N-6 DNA methylase

DNA sequence :
ATGGATCACAGCGTTCACAACAAACTGGTTTCTTTCATCTGGAGCATCGCCGACGACTGCCTGCGGGATGTCTATGTGCG
TGGCAAATACCGTGACGTGATTCTGCCGATGGTGGTGCTGCGCCGTTTAGACACCTTGCTTGAGCCATCGAAAGAAGCGG
TGTTGGAAGAGGTTCGTTTCCAGCAGGAAGAAATGAACGCGGTGGAACTGGATGACGAGCCATTAAAAGCGGCATCGGGT
TATGTGTTTTACAACATCTCGAAATGGACGCTGAAAACCCTACATGCGGCGGCCACCAATAATCAGCAGATCTTGTTGCA
GAATTTTAATGAATACCTGAATGGTTTTAGCGACAACGTCAAAGAGATCGTTGGGCGTTTTAACCTGAAATCGCAGATCC
GCCATATGGCTGAAAAACAGGTGTTGCTCGATGTGGTGGAAAAGTTCATTTCACCCAACATCAACCTGACACCGCAGGAA
TGCGAAGATGCCTCTGGCAACAAACTTCCGGCACTGACCAACCTCGGCATGGGTTATGTGTTTGAAGAGTTGATCCGTAA
ATTTAACGAAGAGAACAACGAAGAGGCGGGCGAACACTTTACCCCGCGTGAAGTGATCGAGCTGATGACGCATCTGGTGT
TTGACCCGCTCAAAGATCAGTTACCGCTCACCATGACCATCTACGACCCCGCCTGTGGCAGTGGTGGCATGCTTACCGAA
TCGCAGAACTTCATTGAAGAGAAATATCCGGCAGTGGGTGCGAGCCGCGACATTCACCTCTACGGCAAAGAGATCAACGA
CGAGACTTACGCGATTTGTAAGTCAGACATGATGATCAAGGGCAATGATCCCGCCAATATCAAAATCGGCTCAACCCTCT
CGACCGATGAATTCTCGCACATGCGGTTCGACTTTATGCTCTCAAACCCGCCCTATGGCAAAAGCTGGGCGTCGGAGCAG
AAAAATATTAAAGAGGGAACCGAGGTCATCGACCCGCGCTTTAAAGTGCAGTTGACTGATTACTGGGGCAAAGTGGATGC
GAAAGGCAGTGATGCCACGCCACGTTCCAGTGACGGGCAATTGCTGTTTCTGATGGAAATGGTCAGCAAGATGAAGGCGC
CGGTTAATGGCACCATTGGCAGCCGTATCGCCTCGGTGCATAACGGTTCAAGCCTGTTTACCGGCGATGCCGGTGGTGGT
GAAAGTAACATCCGCCGTTATCTGATTGAAAACGACATGCTCGAAGCCATCGTGCAACTGCCGAATAACCTCTTCTATAA
CACCGGAATCACCACCTATATCTGGTTGCTGAACAACAACAAACCAGAACGCCGCAAAGGGAAAGTGCAACTGATCGATG
CCAGTCAGTTATTCCGCAAACTGCGTAAAAATTTAGGCAATAAAAACTGCGAATTTGCGCCGGAACATATCGCCGAAATC
ATGCAAACCTACCTTGAATTTAACGAGGTGGAACGCCAGCTTGATGCCAATGGCGACGCCATCGGTTTAGCCAGTAAAAT
TTTCAATAACGAAGATTTCGGTTATTTCAAAGTCACCGTCGAGCGCCCTGATCGCCGTAAAGCGCAGTTCAGCGCCGAGC
GCATTGCGCCGCTGCGTTTTGACAAATCACTCAGCGAAGCGATGGAATATTGCTATGGCGAATATAACGACAAGGTCTAT
CAAGCTGGTTTCTTGGCAGAACAGGGTAAACACATTACCGATTGGTGCGAGAAAAACGACATCAGCTTAAATAACAAAGC
CAAAGAAAAGCTGCTCGATACCGCTTTCTGGCTCAGTTCACGCAAGTTACTCGATGCCGCACAAACGCTGATGGAAGGTG
TGGGTGAGGCTGAGTTTACCGATTTCAATCTGTTTAAAGACAAGGTCGATGCAGCACTGAAAGCCAGCAGTTTGAAACTA
TCCGCGCCCGAAAAGAATGCCATTTTAAATGCCGTGAGCTGGTATGACGAAACCGCAGAAAAAGTGGTGAAGAAGGTGCT
CAAACTCTCTGCTGATAAACTGGCGGAATTACTGGAACTCTATAGCTGCGAAGAGGGCGACCTGCCTGATTTTGGTTATT
ACCCGCATGGCAAGAAAGGCGAATTCGTCACGTATGAAAGCAGCAGCGATTTGCGCGACACCGAGTCCGTGCCACTGAAA
CAGAGCATCTATCAGTATTTCCTTGATGAAGTAAAACCGCATGTTGCTGAAGCTTGGCTGAATATGGAATCGGTGAAGAT
TGGCTGTGAAATCAGCTTTAATAAATATTTCTACCGCCATAAACCACTGCGTAAACTTGAAGCGGTGGCACAAGAAATCA
TTGATCTGGAAAAACAGGCCGATGGTCTGATTGCGCAGATTTTAGGGCTGACAACCCACTCGGCAACGGTATAG

Protein sequence :
MDHSVHNKLVSFIWSIADDCLRDVYVRGKYRDVILPMVVLRRLDTLLEPSKEAVLEEVRFQQEEMNAVELDDEPLKAASG
YVFYNISKWTLKTLHAAATNNQQILLQNFNEYLNGFSDNVKEIVGRFNLKSQIRHMAEKQVLLDVVEKFISPNINLTPQE
CEDASGNKLPALTNLGMGYVFEELIRKFNEENNEEAGEHFTPREVIELMTHLVFDPLKDQLPLTMTIYDPACGSGGMLTE
SQNFIEEKYPAVGASRDIHLYGKEINDETYAICKSDMMIKGNDPANIKIGSTLSTDEFSHMRFDFMLSNPPYGKSWASEQ
KNIKEGTEVIDPRFKVQLTDYWGKVDAKGSDATPRSSDGQLLFLMEMVSKMKAPVNGTIGSRIASVHNGSSLFTGDAGGG
ESNIRRYLIENDMLEAIVQLPNNLFYNTGITTYIWLLNNNKPERRKGKVQLIDASQLFRKLRKNLGNKNCEFAPEHIAEI
MQTYLEFNEVERQLDANGDAIGLASKIFNNEDFGYFKVTVERPDRRKAQFSAERIAPLRFDKSLSEAMEYCYGEYNDKVY
QAGFLAEQGKHITDWCEKNDISLNNKAKEKLLDTAFWLSSRKLLDAAQTLMEGVGEAEFTDFNLFKDKVDAALKASSLKL
SAPEKNAILNAVSWYDETAEKVVKKVLKLSADKLAELLELYSCEEGDLPDFGYYPHGKKGEFVTYESSSDLRDTESVPLK
QSIYQYFLDEVKPHVAEAWLNMESVKIGCEISFNKYFYRHKPLRKLEAVAQEIIDLEKQADGLIAQILGLTTHSATV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC1769 NP_231404.1 DNA methylase HsdM Not tested VPI-2 Protein 0.0 77
VC0395_A1367 YP_001217310.1 DNA methylase HsdM Not tested VPI-2 Protein 0.0 77
VPI2_0017c ACA01834.1 DNA methylase HsdM Not tested VPI-2 Protein 0.0 77

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tola_2273 YP_002893457.1 N-6 DNA methylase VFG1102 Protein 0.0 77