Gene Information

Name : Tola_2270 (Tola_2270)
Accession : YP_002893454.1
Strain : Tolumonas auensis DSM 9187
Genome accession: NC_012691
Putative virulence/resistance : Virulence
Product : type III restriction protein res subunit
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0610
EC number : -
Position : 2489178 - 2492207 bp
Length : 3030 bp
Strand : -
Note : PFAM: type III restriction protein res subunit; protein of unknown function DUF450; SMART: DEAD-like helicase; KEGG: shm:Shewmr7_1787 type III restriction enzyme, res subunit

DNA sequence :
ATGGTAAGCCACACCAACGAACAAGCGCTGGAAGCAGCCATAGAGAAAGCCTTAACCGGCCTGTGTATGGAAGAGGTGAA
AGCGGGTATTAATGAACCTGCTGGTGCTTATATCACCCAGCATAATGGTTTCCAGCAAGGTTTTCCGGCTGATATCGATA
AGCAATATGCCCTCGATTTACGCTTGTTCTGGCAGTTTCTTGAAAACACGCAAGCTGAAGAATTAGCTAAGCTGAAAGTG
CATCATGCCAACGACTGGCAGCGCAAAGTGTTAGAGCGTTTCGACCGTCTGATCAAAAAACACGGTGTGCTGCATCTGCT
CAAGAAAGGCTTAAGTGTGGATGATGCTTACCTGACCCTGATGTATCCGGCGCCACTCGCCAGCAGCAGTCAGGCAGTGA
AACAGAATTTCACCGCGAATATTTTCAGTTGCACCCGACAGGTGTGTTACTCGCAACTCAACCCGTTGCAAGAAATTGAT
ATGGTGCTGTTTATTAACGGCATCCCGCTGGTGACACTCGAACTGAAAAACGCGTGGACGGGTCAAACCGCGCGTTACCA
CGGCCAGAAACAATACCGTGAAGATCGCGACATCAATCAGCCTTTGCTGAACTTTGGCCGTTGTCTGGTGCATATGGCGG
TGGATACCGATGAAGTCTATATGACCACCAAACTGGCTGGTAAAGAGACCTTCTTCCTGCCATTTAATAAAGGCAATAAT
CACGGTCAGGGCAACCCACCCAACCTGTTCGGCCACAAAACGGCCTATCTGTGGGAAGAGGTCTTCACCAAAGTGAGTCT
GGCCAACATCATTCAGCACTTTGTGCGTTTAGATGGCTCATCGAAAGACTTACTCAACAAACGCACCCTGTTCTTCCCCC
GTTATCACCAGCTTGATGTGGTGCGCAGGCTGGTCGATCATGCCGCACATTATGGTGTCGGGCACACCTATCTGATCCAA
CACTCTGCCGGTTCAGGCAAATCAAACTCGATCACATGGGCCGCCTATCAACTGATAGAGGCGTACCCAGCAACCAATGA
TGTGCATGGCAGTCATGGCATCGATCAGCCGTTATTTGATTCTGTGATTGTCGTGACTGACCGCCGCCTGCTAGATAAAC
AGCTCAGAGAGAACATCAAAGAGTTCTCTGAGGTGAAAAACATCATTGCGCCAGCGAATAAATCATCAGAGCTGAAATCG
GCCCTCGAAAATGGCAAGAAGATCATCATCACCACCATCCAGAAATTCCCGTTCATTGTTGATGGCATTAGCGACCTCAG
CGACAAACGTTTTGCCGTGATCATCGACGAAGCGCACAGCTCACAATCGGGCACCGCGCACAGCAAAATGAACGAAGCGA
TGGGGAAAACGGAAGAAGAAGCTGAAGATATTCAAGATCTGATCTTGCAGTCGATGCTATCACGCAAGATGCGGGGCAAT
GCCTCTTATCTGGCGTTCACAGCAACACCGAAGAACACCACATTAGAGAAGTTCGGTCAGCGACAGGAAGATGGCAGCTA
TCGGCCATTCCACCTGTATTCAATGAAACAGGCCATCGAAGAAGGCTTTATTCTGGATGTGGTGGCGAATTACACCACCT
ATCGCAGTTATTACGAAATCAGTAAATCGGTCGAAGACAATCCGCAATTCGACAGTAAAAAGGCACAGAAGAAGTTACGT
GCATATGTCGAAGGCAGTCAGGAAACCATTAATGTGAAAGCCGAAATCATGCTGGAGCATTTTATTCCGCATGTTGTGAA
TGCCAAGAAGCTGAAAGGGAAAGGCAAGGGGATGGTGGTGACGCAGAACATCGTGTCTGCCATTCGTTATTTCAAAGCCA
TTACCAAACAGCTCGACGCGATAGGCAACCCTTTCAAAGTTCTGATCGCATTCTCAGGCACAAAAGAGGTCGATGGCATT
GAATATACCGAAGCTGATCTGAATGGTTTTGCTGAAAATGACACCCGCGATAAATTTGATACTGATGAATACCGCCTGCT
GATTGTCGCGAACAAATATCTGACAGGGTTCGATCAGCCGAAACTCTGCGCGATGTATGTCGATAAAAAACTGGCTGGTG
TGCTTTGCGTGCAAGCATTATCACGTTTGAACCGAGCAGCACCGAAATGGGGTAAGAAAACCGAAGATTTATTTATTCTC
GATTTCTTTAATGCGGTGGATGATATCAAGGCCGCCTTCGACCCGTTTTACACTGCGACATCATTATCACAGGCGACCGA
CATTAACGTGTTGCATGAGCTAAAAGACGCGCTCGATGACGTGGGCGTGTATGAGTGGTATGAAGTCGAAGAGTTCGTTC
AGCGTTATTTCAAAGGCGATGACGCCCAACTACTCAGCGGCATTATCGATGTCGCTGCCGAGCGCTTCAATCAAGAGCTG
GAACTCACCAACGCAGAGCAAGTTGATTTTAAAATCAAAGCCAAGCAGTTCGTAAAAATCTACGGCCAGATGGCATCGAT
TATTCCTTACGACATGTTGTTGTGGGAGAAACTGTTCTGGTTCCTCAAATTCCTGATCCCGAAACTGAAAGTACAAGATC
CAGAAAGCGATCTGCTGGATGAACTGCTCAACTCAGTCGATCTGGCCTCTTACGGCCTACAGCGCGTCAAACTGAATTAC
ACCATTGATTTAGATGCACAAGAAACCACCGTTGACCCGCAGAACCCGAACCCGCGTGGTGGGCATGGCGGTGATGTCGA
GACAGATCCGCTGGACGCGATCATCAATAACTTCAACGAGAAATGGTTCCAAGGCTGGAGCTCAACGCCAGAAGAGCAAA
AAGTGAAGTTCGTGAACATCGCCGACAGCGTGAAAAAACACCCTGATTTCGAAGCGAAATATCAGAACAACCCTGACCCA
ATCAACCGCGAACTGGCATTCGAAAAGATCTTGAAAGAAGTGATGCTACTGCGCCGTAAAGATGAATTGGAGCTCTACAA
GCTGTTCGCCAACGATGCAGCATTTAAAACATCATGGATGCAAAGCGTGCAGAGAATGGTTGGGGTGTGA

Protein sequence :
MVSHTNEQALEAAIEKALTGLCMEEVKAGINEPAGAYITQHNGFQQGFPADIDKQYALDLRLFWQFLENTQAEELAKLKV
HHANDWQRKVLERFDRLIKKHGVLHLLKKGLSVDDAYLTLMYPAPLASSSQAVKQNFTANIFSCTRQVCYSQLNPLQEID
MVLFINGIPLVTLELKNAWTGQTARYHGQKQYREDRDINQPLLNFGRCLVHMAVDTDEVYMTTKLAGKETFFLPFNKGNN
HGQGNPPNLFGHKTAYLWEEVFTKVSLANIIQHFVRLDGSSKDLLNKRTLFFPRYHQLDVVRRLVDHAAHYGVGHTYLIQ
HSAGSGKSNSITWAAYQLIEAYPATNDVHGSHGIDQPLFDSVIVVTDRRLLDKQLRENIKEFSEVKNIIAPANKSSELKS
ALENGKKIIITTIQKFPFIVDGISDLSDKRFAVIIDEAHSSQSGTAHSKMNEAMGKTEEEAEDIQDLILQSMLSRKMRGN
ASYLAFTATPKNTTLEKFGQRQEDGSYRPFHLYSMKQAIEEGFILDVVANYTTYRSYYEISKSVEDNPQFDSKKAQKKLR
AYVEGSQETINVKAEIMLEHFIPHVVNAKKLKGKGKGMVVTQNIVSAIRYFKAITKQLDAIGNPFKVLIAFSGTKEVDGI
EYTEADLNGFAENDTRDKFDTDEYRLLIVANKYLTGFDQPKLCAMYVDKKLAGVLCVQALSRLNRAAPKWGKKTEDLFIL
DFFNAVDDIKAAFDPFYTATSLSQATDINVLHELKDALDDVGVYEWYEVEEFVQRYFKGDDAQLLSGIIDVAAERFNQEL
ELTNAEQVDFKIKAKQFVKIYGQMASIIPYDMLLWEKLFWFLKFLIPKLKVQDPESDLLDELLNSVDLASYGLQRVKLNY
TIDLDAQETTVDPQNPNPRGGHGGDVETDPLDAIINNFNEKWFQGWSSTPEEQKVKFVNIADSVKKHPDFEAKYQNNPDP
INRELAFEKILKEVMLLRRKDELELYKLFANDAAFKTSWMQSVQRMVGV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC1765 NP_231400.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 76
VC0395_A1363 YP_001217306.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 76
VPI2_0013c ACA01830.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 76
SAS0025 YP_042158.1 type I restriction enzyme protein Not tested SCC476 Protein 5e-123 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tola_2270 YP_002893454.1 type III restriction protein res subunit VFG1098 Protein 0.0 76