Gene Information

Name : Tola_0199 (Tola_0199)
Accession : YP_002891415.1
Strain : Tolumonas auensis DSM 9187
Genome accession: NC_012691
Putative virulence/resistance : Virulence
Product : type VI secretion ATPase, ClpV1 family
Function : -
COG functional category : O : Posttranslational modification, protein turnover, chaperones
COG ID : COG0542
EC number : -
Position : 218812 - 221421 bp
Length : 2610 bp
Strand : +
Note : KEGG: pau:PA14_42980 putative ClpA/B-type protease; TIGRFAM: type VI secretion ATPase, ClpV1 family; PFAM: ATPase AAA-2 domain protein; AAA ATPase central domain protein; Clp domain protein; ATPase associated with various cellular activities AAA_5; SMART:

DNA sequence :
ATGATAACAATTAAATTAGATGAGCTGATTAGTGCTCTGGCTCCTGCTGAAAAGCAGGCGCTGGAACAGGCAGCAAATTA
CTGTGTAGCGCGGGCGGGAAGTGAAATATTAGTTGAAGACTATTTGCTCGCGTTGCTGGATGGTTCGGATAGCTTCCTGA
ATAAAGTGTTGCTGCAATTTTCACTGAGTGCTGACACGTTGCGTACCATTCTGCTGGAGTCCGTCGGATACCAGAAAACA
GAAAGCCGGAATCCGGTATTCTCAACGTTGTTAGTTAACTGGCTGGAAGAAGCTTTTCTGATAACAAAACTGCAACTGCA
ACAGTCTTCTATTGCTTGTCTTGCATTATTACTGGCGATTCAGGATCGACCGGAGAAATACGCACGTACCCGCTATGGTC
AGTTTTTCTCTGATATTCCCCGCGAAAAGCTGATGGCTTTAATGGATGGTTTGCAGGCGGAAGTTCTGTCTGCCATGTCA
GTTTCTGGTGCGGGGAGTGCTCTGCAGCAGTTCACCAAAAGCTACACCCAGCTGGCAAGAGATGGAAAGTTAGATCCGGT
GTTGTGCCGGGATGAAGAGATCCGCCAGATTGTGGATATTTTATCGCGTCGTCGTAAAAACAATCCGATTCTGGTTGGTG
AAGCCGGGGTTGGTAAAACCGCCGTAGTGGAGGGGTTGGCACAGCACGTCATCGCGGGGCTGGTGCCACCTGCATTGACG
GGGATTGAACTGCTGGAACTGGATATGGGGTTGTTGCAAGCCGGAGCCAGTGTAAAGGGTGAATTTGAGCGACGTCTTAA
GGCGGTCATTGATGAGGTCAACGCATCACCTAAGCCAATTGTGTTATTTATCGATGAAGCACATACCCTGATAGGTGCCG
GTGGTCAGGCGGGTGGCGGTGATGCGGCTAATCTATTGAAACCGGCTCTGGCTCGCGGAACTCTGCGTACACTGGCTGCA
ACTACATGGTCAGAATACAAAAAATATTTTGAGAAAGACCCTGCTCTGGCACGTCGGTTCCAGCCCGTTCAGGTACTGGA
ACCGACTATTACTCAGGCAACAACCATCCTGCGCGGCTTAGTCTCCCGTTACGAAGTCGCGCATGATGTCTATCTGCGCG
ATGATGCTGTCGTGACAGCCGCAGAATTATCCGCGCGTTATTTATCAGGCCGTCAGTTGCCGGATAAGGCAATTGATTTG
CTGGATACTGCATGTGCCCGGGTAAAAAACAGCCAGCATGCAACCCCCATACGTCTGCAGGCCATCGCGACTTCCCTGGC
TGAAAAACAGCGTTTGCAGACGGCATTATTGCGTGATCAGCAGCATGGTTTGCCTGTCGAACCAGGACAACTGGCTGCTC
TGGAACGTCAGATCCCGCAGTTGGAACAAGAACACGCTGAATTGTCTGCACACTGGCAAGAGCAAAAAACGCTGGTGGCA
CAGTTGGTTGCCAGTCGTAATCCTGAGGTTTCATCACTGCTGTCTGCAGCAGATAATCAGTCGGAAATTACCGCTGAGCT
TGTGGATGTGGATGTCGAGGCTCCTGAAACTGTTCTGGCCGCATTAGCCGAATTGCAGCAGTCACAAGGCATTCTGATCC
ATCATGAAGTGACACCACAAGTTGTGGCGGAAGTTATTTCCAGTTGGACAGCCATTCCGGTAGAAAATCTGATTGAAGCG
CCATCTGCACGGGTTGTTGAATTCGCCGATCGACTACGAGCCCGTATCCGTGGTCAGGCTCATGCGGTTTCATTAATTGA
ACGAGCTGTAAAAGCCACTGCGGCCGGGTTGGGTAATCCTCTGGCGCCAACAGGGGTGTTCCTGCTGGCAGGGCCAAGTG
GTGTTGGTAAAACTGAAACCGCGGTTGCTGTTGCCGAACTGCTGTATGGCGGTGAACGTTTTCTGACCACGATTAATATG
TCTGAATTTCAGGAAAAGCATTCGCTTTCCCGTCTGATCGGTTCTCCACCCGGATATGTGGGGTATGGTGAAGGTGGAAT
ATTAACCGAAGCCGTCCGTCAACGACCATATTCAGTGATTTTGCTGGATGAGGTGGAAAAAGCTGACCCTGACATTCTGA
ATCTTTTTTATCAAATCTTTGATAAAGGCATCGCCAATGATGGTGAAGGCCGGGTAATCAATTTCCGCAATACCCTGATT
TTTATGACATCAAATCTGGCATCAGCAGAGATCAGCTCGCTGGCAGCCGGTGAAACGCCGGTAATGACCGATTCGATAAC
AGAAACGATCCGTCCGGTGCTGGCTAAACATTTTAAACCTGCATTGCTGGCACGCATGAACATTATTCCTTATCTGCCAA
TTGCAAACGATGTGATGCATGAACTGGTGTTGCTGCGCTTGCAGAAACTGTCGCAACGGATTGCATCCCGCGGAATCGCG
TTGACCTTTGACGCTGCGGTGGTAACGGCCATTGCCGAGAGCTGCACCCGGACTGACACCGGTGCCCGGAATATCGATTT
TCTGGTTAATAACTATCTGTTACCGGCAATTTCAGATCGCATTCTGAAAGCGCTGACGCAGCAGGAAAATATCCGCGGCA
TTCGGGTAGTACAGGATGAATACGGTAATTTTGAACTGATTATCGGGTAA

Protein sequence :
MITIKLDELISALAPAEKQALEQAANYCVARAGSEILVEDYLLALLDGSDSFLNKVLLQFSLSADTLRTILLESVGYQKT
ESRNPVFSTLLVNWLEEAFLITKLQLQQSSIACLALLLAIQDRPEKYARTRYGQFFSDIPREKLMALMDGLQAEVLSAMS
VSGAGSALQQFTKSYTQLARDGKLDPVLCRDEEIRQIVDILSRRRKNNPILVGEAGVGKTAVVEGLAQHVIAGLVPPALT
GIELLELDMGLLQAGASVKGEFERRLKAVIDEVNASPKPIVLFIDEAHTLIGAGGQAGGGDAANLLKPALARGTLRTLAA
TTWSEYKKYFEKDPALARRFQPVQVLEPTITQATTILRGLVSRYEVAHDVYLRDDAVVTAAELSARYLSGRQLPDKAIDL
LDTACARVKNSQHATPIRLQAIATSLAEKQRLQTALLRDQQHGLPVEPGQLAALERQIPQLEQEHAELSAHWQEQKTLVA
QLVASRNPEVSSLLSAADNQSEITAELVDVDVEAPETVLAALAELQQSQGILIHHEVTPQVVAEVISSWTAIPVENLIEA
PSARVVEFADRLRARIRGQAHAVSLIERAVKATAAGLGNPLAPTGVFLLAGPSGVGKTETAVAVAELLYGGERFLTTINM
SEFQEKHSLSRLIGSPPGYVGYGEGGILTEAVRQRPYSVILLDEVEKADPDILNLFYQIFDKGIANDGEGRVINFRNTLI
FMTSNLASAEISSLAAGETPVMTDSITETIRPVLAKHFKPALLARMNIIPYLPIANDVMHELVLLRLQKLSQRIASRGIA
LTFDAAVVTAIAESCTRTDTGARNIDFLVNNYLLPAISDRILKALTQQENIRGIRVVQDEYGNFELIIG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY0294 NP_454876.1 ClpB-like protein Not tested SPI-6 Protein 5e-122 46
aec27 YP_851418.1 ATPase Not tested PAI II APEC-O1 Protein 5e-146 45
aec27 AAQ96721.1 Aec27 Not tested AGI-1 Protein 3e-146 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tola_0199 YP_002891415.1 type VI secretion ATPase, ClpV1 family VFG2076 Protein 2e-146 49
Tola_0199 YP_002891415.1 type VI secretion ATPase, ClpV1 family VFG2084 Protein 1e-158 48