Gene Information

Name : PFLU4949 (PFLU4949)
Accession : YP_002874455.1
Strain : Pseudomonas fluorescens SBW25
Genome accession: NC_012660
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 5429472 - 5430923 bp
Length : 1452 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGTCCGTCCGTCGTACCAAAATCGTCGCCACCCTTGGCCCGGCCAGCAACTCGCCGGAAGTCCTCGAACAGCTGATTCT
GGCTGGTTTGGACGTTGCCCGTCTGAACTTCTCCCACGGCACCCCGGACGAGCACAAGGCTCGCGCCAAGCTGGTGCGTG
AACTGGCTGCCAAGCACGGCCGCTTCGTGGCCCTGCTGGGTGACCTGCAAGGCCCGAAGATCCGTATCGCCAAATTCGCC
AACAAGCGGATCGAGCTGAAAATCGGTGACACCTTCACCTTTTCCACCAGCCACCCGCTGACCGAGGGCAACCAGCACGT
CGTGGGTATCGACTACCCGGACCTGGTCAAGGACTGCGGCGTCGGTGACGAGTTGCTGCTGGACGATGGCCGCGTGGTCA
TGCGCGTCGACACCGCCACCGGCACCGAGCTGATCTGCACCGTGACCATCGGCGGCCCACTGTCCGACCACAAAGGCATC
AACCGCCGTGGCGGCGGCCTGACGGCCCCGGCATTGACGGAAAAAGACAAGGCTGACATCAAGCTCGCCGCCGAAATGGA
AGTGGACTACCTCGCCGTGTCCTTCCCGCGCGACGCAGCCGACATGGAATACGCCCGTAAACTGCGCGACGAAGCCGGCG
GCACCGCCTGGCTGGTCGCGAAGATCGAACGCGCCGAAGCCGTGGCCGACGACGAAACCCTCGACGGCCTGATCAAGGCT
TCCGACGCGGTGATGGTTGCCCGTGGCGACCTCGGCGTGGAAATCGGTGATGCCGAGCTGATCGGTATCCAGAAGAAGAT
CATCCTGCACGCACGCCGCCACAACAAAGCGGTGATCGTGGCGACCCAGATGATGGAGTCGATGATCCAGAACCCGATGC
CGACCCGTGCCGAAGTGTCCGACGTGGCCAACGCCGTGCTCGACTACACCGACGCCGTGATGCTCTCGGCTGAAAGTGCC
GCCGGCCCGTATCCGCTGGAAGCCGTACAAGCCATGGCGCGTATCTGCATCGGCGCTGAAAAGCACCCGACCAGCAAGAC
CTCCAGCCACCGTATCGGCAAAGAGTTCGAAAGCTGCGACCAGAGTATCGCCCTGGCGGCCATGTACACCGCCAACCACT
TCCCAGGTGTGAAGGCGATCATTGCGTTGACCGAAAGTGGCTACACGCCATTGATCATGTCGCGCATCCGTTCGTCGGTG
CCGATCTACGCGTTCTCCCCGCACCGCGAAACCCAGGCCCGCGCGGCCATGTTCCGTGGCGTGTACACCGTCCCGTTCGA
CCCGGCCTCGTTGCCGCCGCACGAAGTCAGCCAGGCTGCCATTGATGAGCTGATCAAGCGTGGTGTTGTAGAGAAAGGCG
ACTGGGTCATCCTGACCAAGGGCGACAGCTACCACACCATCGGCGGTACCAACGGCATGAAGATTCTGCACGTTGGCGAC
CCAATGGTCTGA

Protein sequence :
MSVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRELAAKHGRFVALLGDLQGPKIRIAKFA
NKRIELKIGDTFTFSTSHPLTEGNQHVVGIDYPDLVKDCGVGDELLLDDGRVVMRVDTATGTELICTVTIGGPLSDHKGI
NRRGGGLTAPALTEKDKADIKLAAEMEVDYLAVSFPRDAADMEYARKLRDEAGGTAWLVAKIERAEAVADDETLDGLIKA
SDAVMVARGDLGVEIGDAELIGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGPYPLEAVQAMARICIGAEKHPTSKTSSHRIGKEFESCDQSIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSV
PIYAFSPHRETQARAAMFRGVYTVPFDPASLPPHEVSQAAIDELIKRGVVEKGDWVILTKGDSYHTIGGTNGMKILHVGD
PMV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 2e-79 46
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-61 46
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-61 45
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-61 45
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-61 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
PFLU4949 YP_002874455.1 pyruvate kinase VFG0479 Protein 5e-62 45