Gene Information

Name : fliI (PFLU4436)
Accession : YP_002873969.1
Strain : Pseudomonas fluorescens SBW25
Genome accession: NC_012660
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 4898823 - 4900181 bp
Length : 1359 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGCGCCTTGATCGCACCAGCTTCGCCAAGCGCTTGAGCGGTTACGCCGAAGCCACATCGTTGCCCGGCCAGCCGATCCT
CGAAGGGCGCCTGTTGCGCATGGTCGGCCTGACCCTCGAAGCCGAAGGCCTGCGCGCCGCCATGGGCAGCCGCTGCATGG
TCATCAACGACGACAGCTACCACCCGGTGCAAGTCGAAGCCGAAGTGATGGGTTTTTCCGGCAACAAGATTTTCCTGATG
CCCGTCGGCAGCCTGGCGGGCATTGCCCCTGGCGCCCGCGTGGTGCCATTGGCCGACACCGGCCGCCTGCCGATGGGCAT
GAGCATGCTCGGCCGCGTACTGGACGGCGCCGGTCGCGCGCTGGATGGCAAGGGCGGCATGAAGGCCGAAGATTGGGTGC
CGATGGACGGCCCCACCATCAACCCGCTCAACCGTAACCCCATCAGCGTGCCGCTGGACGTGGGCATTCGCAGCATCAAC
GGATTATTGACGGTCGGTCGCGGCCAGCGTCTGGGCCTGTTCGCCGGTACCGGCGTGGGTAAGTCGGTGTTGCTGGGCAT
GATGACGCGCTTTACCGAGGCCGACATCATTGTGGTGGGCCTGATCGGTGAGCGGGGCCGTGAGGTGAAGGAATTCATCG
AGCACAGCCTCGGCGAAGAAGGCCTCAAGCGCTCCGTCGTGGTCGCCTCGCCGGCGGACGATGCGCCGCTGATGCGTTTG
CGCGCCGCGATGTACTGCACGCGCATCGCTGAGTATTTTCGCGACAAGGGCAAGAACGTCCTGTTGCTGATGGACTCGCT
CACGCGTTTTGCCCAGGCCCAGCGGGAAATCGCCCTGGCCATTGGCGAACCGCCTGCGACCAAGGGTTACCCGCCGTCGG
TGTTCGCCAAGTTGCCCAAACTGGTGGAACGCGCCGGTAATGCCGAGGCGGGCGGTGGCTCGATCACCGCGTTCTATACG
GTGCTGTCCGAAGGCGATGACCAGCAGGACCCGATTGCCGACTCGGCGCGGGGCGTGCTCGACGGCCACATTGTGCTGTC
GCGGCGTCTGGCCGAAGAAGGGCACTACCCGGCCATCGATATCGAAGCGTCCATCAGCCGGGTGATGCCGGCGGTGGTCA
CCCCGGAACACATGGCCCGCGCGCAACACTTCAAGCAACTGTGGTCGCGCTACCAACAAAGCCGCGACTTGATCAGTGTC
GGCGCCTACGTGGCCGGCGGTGATCGCGACACCGACCTGGCCATCGCCCTGCAACCGCAACTGGTGACCTACTTGCGCCA
AGGCCTCAACGACAAGATCAGCATGGGCGAAAGCGAAGCGCACCTGCAGTCGATCTTCGCCCCCGCGCCGGGCGGCTAA

Protein sequence :
MRLDRTSFAKRLSGYAEATSLPGQPILEGRLLRMVGLTLEAEGLRAAMGSRCMVINDDSYHPVQVEAEVMGFSGNKIFLM
PVGSLAGIAPGARVVPLADTGRLPMGMSMLGRVLDGAGRALDGKGGMKAEDWVPMDGPTINPLNRNPISVPLDVGIRSIN
GLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTEADIIVVGLIGERGREVKEFIEHSLGEEGLKRSVVVASPADDAPLMRL
RAAMYCTRIAEYFRDKGKNVLLLMDSLTRFAQAQREIALAIGEPPATKGYPPSVFAKLPKLVERAGNAEAGGGSITAFYT
VLSEGDDQQDPIADSARGVLDGHIVLSRRLAEEGHYPAIDIEASISRVMPAVVTPEHMARAQHFKQLWSRYQQSRDLISV
GAYVAGGDRDTDLAIALQPQLVTYLRQGLNDKISMGESEAHLQSIFAPAPGG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-78 51
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-78 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-78 51
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-78 51
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-79 51
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-85 49
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-75 48
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-75 48
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-76 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-86 48
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-86 48
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-88 48
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-87 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-84 48
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 3e-81 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-88 47
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-88 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-88 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-88 47
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-71 47
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-75 47
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 5e-72 47
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-72 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 6e-69 46
escN AAK26715.1 EscN Virulence LEE Protein 9e-74 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-74 46
escN AAL57542.1 EscN Virulence LEE Protein 9e-74 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-74 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 4e-74 46
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-74 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-74 46
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-71 46
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-71 46
unnamed AAL06369.1 EscN Virulence LEE Protein 7e-73 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-74 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-74 44
escN AAC31513.1 L0034 Virulence LEE Protein 9e-75 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 9e-75 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-71 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-68 43
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-68 43
escN AAC38383.1 EscN Virulence LEE Protein 1e-74 43
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-67 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 6e-66 42
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-61 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-61 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-61 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-61 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_002873969.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 87
fliI YP_002873969.1 flagellum-specific ATP synthase VFG2499 Protein 3e-126 63
fliI YP_002873969.1 flagellum-specific ATP synthase VFG2331 Protein 6e-125 62
fliI YP_002873969.1 flagellum-specific ATP synthase VFG0515 Protein 7e-79 51
fliI YP_002873969.1 flagellum-specific ATP synthase VFG0048 Protein 2e-91 47
fliI YP_002873969.1 flagellum-specific ATP synthase VFG2144 Protein 9e-90 47
fliI YP_002873969.1 flagellum-specific ATP synthase VFG0192 Protein 1e-87 47
fliI YP_002873969.1 flagellum-specific ATP synthase VFG0390 Protein 1e-86 46
fliI YP_002873969.1 flagellum-specific ATP synthase VFG0812 Protein 5e-75 44
fliI YP_002873969.1 flagellum-specific ATP synthase VFG0730 Protein 8e-75 43
fliI YP_002873969.1 flagellum-specific ATP synthase VFG0555 Protein 9e-62 41