Gene Information

Name : cvrA (PFLU0446)
Accession : YP_002870122.1
Strain : Pseudomonas fluorescens SBW25
Genome accession: NC_012660
Putative virulence/resistance : Unknown
Product : potassium/proton antiporter
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG3263
EC number : -
Position : 494074 - 495816 bp
Length : 1743 bp
Strand : -
Note : the Vibrio parahaemolyticus gene VP2867 was found to be a potassium/proton antiporter; can rapidly extrude potassium against a potassium gradient at alkaline pH when cloned and expressed in Escherichia coli

DNA sequence :
TTGAATGCGACGACCATCAACAGCCTGTTCTTGATCGGCGCGTTGCTGGTGAGTGCGAGCATTCTGGTAAGTTCTCTGTC
GTCCCGCCTGGGCATCCCGATCCTGGTGATCATCCTGGCCGTGGGCATGGTCGCCGGCGTCGATGGCGGCGGCATCATCT
TCGATAACTACCCGACCGCCTATCTGGTGGGCAACCTCGCGTTGGCAGTGATACTGCTCGACGGTGGCCTGCGCACGCGG
GTGGCGAGTTTCCGCGTGGCACTCTGGCCGGCGTTGTCGCTGGCCACAGTGGGGGTGTTGATTACCACCGGCCTCACCGG
CATGGCGGCAGCCTGGCTGTTTGACCTCAATATCATCCAGGGGCTGTTGATCGGCGCGATTGTCGGTTCCACGGACGCCG
CGGCGGTGTTCTCGTTGCTCGGCGGCAAGGGCCTGAACGAACGGGTCACCGCCAGCCTGGAAATCGAATCCGGCAGCAAC
GACCCGATGGCGGTGTTCCTCACCGTCACCTTGATCGACATGCTCGCCAGCGGCCAGACCGGCCTGCACTGGAGCCTGCT
GACGCACCTGGTGCGCGAGTTCGGCATCGGCGGCGTGGTCGGCCTGGGTGGCGGCTGGCTGATGCTGCAGATGGTCAACC
GCATCAACCTGGCCACCGGCCTGTACCCGATCCTGGTGATCGCCGGTGGCCTGTTCGTGTTTGCCGTGACCAACGCCCTG
CACGGCAGCGGCTTCCTCGCCGTGTACCTGTGCGGCCTGGTGATCGGCAACCGCCCGGTGCGCAGCCGTCATGGCATTTT
GCATATGCTCGACGGCATGGCCTGGCTGGCGCAGATCGGCATGTTCCTGGTGCTGGGCCTGCTGGTTACACCCCACGACT
TGCTGCCTATCGCGCTGCCGGCCCTGGGCCTGGCGCTGTGGATGATCCTGTTTGCGCGGCCGCTGTCGGTGATGGTCGGC
CTGCTGCCATTCAAGGCGTTCCACGGCCGCGAAAAAGCCTTTATCGCCTGGGTCGGCCTGCGCGGCGCGGTACCGATCAT
TCTGGCGGTGTTCCCCCTGATGGCCGGGCTGCCGCATGCGCAGCTGTACTTCAACCTGGCGTTCTTTATCGTGCTGGTCT
CGCTGCTGGTGCAGGGCACCAGCCTGCCGTGGGTGGCCAAGCTGCTCAAGGTCACCGTACCGCCGGACCCGGCGCCGATT
TCCCGCGCAGCACTGGAAGTGCATGTCACCAGCGAGTGGGAGCTGTTCGTCTACCGCCTCGGTGCGGAAAAATGGTGCAT
CGGCGCCGCCCTGCGCGAACTGAAAATGCCTGAAGGCACGCGGATCGCCGCGCTGTTCCGTGGCCAGCAACTGCTCCATC
CGTCGGGTAGTACCGTGCTGGAAGTCGGCGATTTGCTCTGCGTGATCGGCCATGAACACAACCTGCCGGCCCTCGGCAAA
CTGTTCAGCCAGGCACCGCAACGCGGTTTGGATTTACGCTTCTTCGGCGACTTCGTACTCGAAGGCGACGCCCAGCTGGG
CGCGGTTTCCGCCCTGTACGGGCTGAAGCTGGAAGGCATCGACCCGGACATGCCACTGAGCCGCTTCATCACCCAGAAAG
TTGGGGGGGCGCCAGTCGTCGGCGACCAGGTGGAGTGGAACAACACCATCTGGACCGTCGCGGTCATGGACGGGAACAAG
ATTGGCAAAGTCGGCGTCAGATTCCCCGAAGGAAGTCGCCCGGGCCCCGGACTCTTCCTCTAA

Protein sequence :
MNATTINSLFLIGALLVSASILVSSLSSRLGIPILVIILAVGMVAGVDGGGIIFDNYPTAYLVGNLALAVILLDGGLRTR
VASFRVALWPALSLATVGVLITTGLTGMAAAWLFDLNIIQGLLIGAIVGSTDAAAVFSLLGGKGLNERVTASLEIESGSN
DPMAVFLTVTLIDMLASGQTGLHWSLLTHLVREFGIGGVVGLGGGWLMLQMVNRINLATGLYPILVIAGGLFVFAVTNAL
HGSGFLAVYLCGLVIGNRPVRSRHGILHMLDGMAWLAQIGMFLVLGLLVTPHDLLPIALPALGLALWMILFARPLSVMVG
LLPFKAFHGREKAFIAWVGLRGAVPIILAVFPLMAGLPHAQLYFNLAFFIVLVSLLVQGTSLPWVAKLLKVTVPPDPAPI
SRAALEVHVTSEWELFVYRLGAEKWCIGAALRELKMPEGTRIAALFRGQQLLHPSGSTVLEVGDLLCVIGHEHNLPALGK
LFSQAPQRGLDLRFFGDFVLEGDAQLGAVSALYGLKLEGIDPDMPLSRFITQKVGGAPVVGDQVEWNNTIWTVAVMDGNK
IGKVGVRFPEGSRPGPGLFL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
f536 AAC61706.1 F536 Not tested PAI I CFT073 Protein 2e-146 65
unnamed AAB61290.1 unknown Not tested PAI I CFT073 Protein 2e-146 65